A Simple, Low Cost, Sensitive, and Portable Electrochemical Immunochromatography Sensing Device to Measure Estrone-3-Sulfate

Abstract

In livestock production, point-of-care testing (POCT) technology that enables easy on-site analysis of sex hormones is desired to improve reproductive efficiency. In this context, low-molecular-weight endogenous steroids are particularly important for perinatal management. Therefore, we attempted to use a simple method that combines electrochemical techniques with immunochromatography to measure estrone-3-sulfate (E1S), one of the low-molecular-weight endogenous steroids that is an estrogen ester. The limit of detection (LOD) for E1S achieved by electrochemical immunochromatography was 570.5 ng/mL, which was one to two orders of magnitude lower than that of small molecule compounds analyzed by other POCT techniques (Primpray et al., Anal. Chim. Acta, 2019). In addition, it was indicated by a colorimetric analysis that the sensitivity of the electrochemical immunochromatographic technique could be enhanced by improving the method of application of the antibodies on the nitrocellulose membrane and the contact between the electrochemical detector and the nitrocellulose membrane.

Electrochemical studies of lateral flow assay test results for procalcitonin detection

“…These results confirmed the accuracy of the currently presented cyclic voltammetry (CV) studies. From observing CV curves, it was found that the pattern of oxidation currents reduces with increasing concentration and proved that the responses are concentration-dependent [24][25][26][27]. Here, the LFA strip test results are validated by the quantitative and qualitative determination of PCT.…”

Portable Electrochemical Immunochromatography Platform for Point-of-care Testing and Comparison with Enzyme-linked Immunosorbent Assay

“…For the electrochemical detection of IgG, we used our previously developed electrochemical immunochromatography platform. (10,11) The platform consists of a nitrocellulose membrane, absorbent pad, electrochemical detector, and two polymethyl methacrylate (PMMA) plates (Fig. 1).…”

“…Therefore, we previously developed an electrochemical immunochromatography platform to investigate the effects of the contact pressure and flow rate between an electrode and a nitrocellulose membrane. (9)(10)(11) Usually, electrodes are printed on a paper when an electrochemical method is introduced into µPADs. (7,12,13) In this case, the electrodes are disposable along with the paper, increasing their cost of use.…”

Integrated electrochemical lateral flow immunoassays (eLFIAs): recent advances

“…Although an electrochemical analyzer is required to obtain quantitative test results, 12 the printed electrode fills the porous membrane and obstructs the flow caused by capillary force. 13 Lee et al 14 first † These authors contributed equally and should be regarded as co-first authors of the article. a School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, China.…”