Integrated electrochemical lateral flow immunoassays (eLFIAs): recent advances

Cheng, Jie; Yang, Guopan; Jie, Guo; Liu, Shan; Guo, Jinhong

doi:10.1039/d1an01478a

Cited by 27 publications

(18 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The versatility of current electrochemical substrates in terms of use (reusable or disposable , ), design, fabrication materials (paper, sustainable, edible, plastic, textile, and polymeric), and properties (superwettable, flexible, and stretchable) should be highlighted. Electrochemical affinity bioassays have also been successfully integrated into microfluidic (Figure b) and lateral flow (eLFA) devices (Figure c). , …”

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

“…Electrochemical affinity bioassays have also been successfully integrated into microfluidic 25 (Figure 3b) and lateral flow (eLFA) devices (Figure 3c). 26,27 Moreover, advances in microfabrication and microfluidic tecnologies 28 have led to the development of integrated devices able to unify the sample collection, preparation, analysis, and postprocessing (known as "sample-in-answer-out" type of devices 29 ) or able to obtain multiplexed 30,31 and/or multiomic information in a minimally invasive manner.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

View full text Add to dashboard Cite

Electrochemical affinity biosensors are evolving at breakneck speed, strengthening and colonizing more and more niches and drawing unimaginable roadmaps that increasingly make them protagonists of our daily lives. They achieve this by combining their intrinsic attributes with those acquired by leveraging the significant advances that occurred in (nano)materials technology, bio(nano)materials and nature-inspired receptors, gene editing and amplification technologies, and signal detection and processing techniques. The aim of this Perspective is to provide, with the support of recent representative and illustrative literature, an updated and critical view of the repertoire of opportunities, innovations, and applications offered by electrochemical affinity biosensors fueled by the key alliances indicated. In addition, the imminent challenges that these biodevices must face and the new directions in which they are envisioned as key players are discussed.

show abstract

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

mentioning

confidence: 99%

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

View full text Add to dashboard Cite

show abstract

“…An alternative strategy to obtain true quantitative outputs is the integration of electrochemical transduction into the LFA strips. 12,13 Printing metal electrodes on nitrocellulose membranes using commercial inks has proven to be challenging however, due to the use of organic solvents in their formulations (needed to dissolve the polymer binder) which damage the delicate membrane. Instead of direct printing of metal inks onto the nitrocellulose membrane, attempts were made to attach external electrodes to the detection area of the LFA.…”

Section: Introductionmentioning

confidence: 99%

Facilitating Electrochemical Lateral Flow Assay using NFC-Enabled Potentiostat and Nitrocellulose-based Metal Electrodes

Gonzalez‐Macia

Liu

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Rapid detection of pathogens at the point-of-need is crucial for preventing the spread of human, animal and plant diseases which can have devastating consequences both on the lives and livelihood of billions of people. Colorimetric, lateral flow assays consisting of a nitrocellulose membrane, are the preferred format today for low-cost on-site detection of pathogens. This assay format has, however, historically suffered from poor analytical performance and is not compatible with digital technologies. In this work, we report the development of a new class of digital diagnostics platform for precision point-of-need testing. This new versatile platform consists of two important innovations: i) A wireless and batteryless, microcontroller-based, low-cost Near Field Communication (NFC)-enabled potentiostat that brings high performance electroanalytical techniques (cyclic voltammetry, chronoamperometry, square wave voltammetry) to the field. The NFC-potentiostat can be operated with a mobile app by minimally trained users; ii) A new approach for producing nitrocellulose membranes with integrated electrodes that facilitate high performance electrochemical detection at the point-of-need. We produced an integrated system housed in a 3D-printed phone case and demonstrated its used for the detection of Maize Mosaic Virus (MMV), a plant pathogen, as a proof-of-concept application.

show abstract

“…Most eLFIAs developed in a single device are established by assembling a screen-printed electrode into the LFIA strip . However, electrodes prepared by a screen-printing method still require masks, toxic chemicals, and expensive inks, leading to waste disposal requirements and environmental damage .…”

mentioning

confidence: 99%

“…Most eLFIAs developed in a single device are established by assembling a screen-printed electrode into the LFIA strip. 18 However, electrodes prepared by a screen-printing method still require masks, toxic chemicals, and expensive inks, leading to waste disposal requirements and environmental damage. 19 As a new alternative electrode fabrication method, laser engraving has been introduced as an interesting approach based on CO 2 laser irradiation of sp 3 carbons found in carbon-rich precursors [e.g., polyimide (PI) and wood] to induce photochemical and thermal conversion into highly conductive sp 2 -hybridized carbons found in graphene.…”

mentioning

confidence: 99%

Dual Colorimetric/Electrochemical Detection of Salmonella typhimurium Using a Laser-Induced Graphene Integrated Lateral Flow Immunoassay Strip

Preechakasedkit,

Panphut,

Lomae

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food safety for producers and consumers. Unfortunately, current detection techniques still suffer from time-consuming operations and the need for highly skilled personnel. Here, we introduce a highly sensitive dual colorimetric/electrochemical detection approach for Salmonella enterica serovar typhimurium (S. typhimurium) based on a laser-induced graphene-integrated lateral flow immunoassay (LIG−LFIA) strip. The LIG electrode was fabricated by laser engraving on a polyimide tape containing a pseudo silver/silver chloride reference electrode from silver sintering and chlorination. Using double-sided tape inserted into the strip, automatic sequential reagent delivery was enabled for the dual-mode signal readout by single-sample loading. A gold-deposited gold nanoparticle strategy was first employed to simultaneously obtain a colorimetric signal for early screening and a signal turn-on electrochemical response for high-sensitivity and -quantitative analysis. A superior performance of the strip was established, characterized by a short analysis time (12 min assay +15 min sample preparation), a broad working concentration range (1 cfu/10 mL to 10 8 cfu/mL), and the lowest limit of detection (1 ± 0.5 cfu/10 mL; mean ± standard deviation, n = 3) among reported multimode S. typhimurium detection schemes. The strip was successfully applied in the analysis of various food products without any bacterial enrichment or amplification required, and the results were comparable to those of the standard culture method.

show abstract

Integrated electrochemical lateral flow immunoassays (eLFIAs): recent advances

Abstract: With the rise of electroactive nano-biomaterials and flexible electrodes, there has been a growing interest in the integrated electrochemical lateral flow immunoassay (eLFIA). eLFIA combines electrochemical nanotags and the integrated...

Cited by 27 publications

References 76 publications

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Facilitating Electrochemical Lateral Flow Assay using NFC-Enabled Potentiostat and Nitrocellulose-based Metal Electrodes

Dual Colorimetric/Electrochemical Detection of Salmonella typhimurium Using a Laser-Induced Graphene Integrated Lateral Flow Immunoassay Strip

Contact Info

Product

Resources

About