A simple liquid chromatography-high resolution mass spectrometry method for the determination of glyphosate and aminomethylphosphonic acid in human urine using cold-induced phase separation and hydrophilic pipette tip solid-phase extraction

Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis

“…In other words, an ACN/water-based “in situ LLE” can be well realized through a simple cooling down process. In the past few years, this mechanism (naming as “cold-induced phase separation (CIPS),” “low-temperature-induced phase separation,” “homogeneous liquid-liquid microextraction,” “low-temperature partition,” and others) has been mainly utilized for drug/pesticide-residue analysis in food/environmental science ( 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ). However, its applicability for pretreatment of biotargets remains to be clarified.…”

An analytical method for the determination of glyphosate and aminomethylphosphoric acid using an anionic polar pesticide column and the application in urine and serum from glyphosate poisoning patients

“…GLY (purity >98.6%) and AMPA (purity >99%) were purchased from Dr Ehrenstorfer GmbH (Augsburg, Germany). The isotope internal standards (ISs) 13 C 2 , 15 N-GLY (purity >97.3%) and 13 C, 15 N-AMPA (purity >99.0%) were purchased from ANPEL Laboratory Technology Co., Ltd (Shanghai, China) and Toronto Research Chemistry Company (North York, Canada).…”

Analytical methods to analyze pesticides and herbicides

“…This method required one single analytical run without need for preconcentration and obtained estimated LODs of 0.1-5 ng/L, accuracy of 86%-114%, and RSD < 18% for all analytes. Chen, Miao, Miao, Zhao, and Wu (2019) proposed an LC-HRMS approach to determine glyphosate and aminomethylphosphonic acid (a glyphosate metabolite) via hydrophilic pipette tip solid-phase extraction (PTSPE) following cold-induced phase separation (CIPS). Using the single ion monitoring mode, this method avoided tedious derivatization, improving method selectivity and sensitivity.…”

“…Chen, Miao, Miao, Zhao, and Wu (2019) proposed an LC‐HRMS approach to determine glyphosate and aminomethylphosphonic acid (a glyphosate metabolite) via hydrophilic pipette tip solid‐phase extraction (PTSPE) following cold‐induced phase separation (CIPS). Using the single ion monitoring mode, this method avoided tedious derivatization, improving method selectivity and sensitivity.…”