An analytical method for the determination of glyphosate and aminomethylphosphoric acid using an anionic polar pesticide column and the application in urine and serum from glyphosate poisoning patients

Zhang, Hao; Dou, Jianrui; Miao, Runfeng; Hu, Jian; Huo, Zongli; Zhang, Feng; Ji, Wenliang

doi:10.1039/d3ay00039g

Cited by 2 publications

(2 citation statements)

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“…Several analytical methods have been developed for measuring GlyP in human urine, including AMPA. These include the use of enzyme-linked immunosorbent assays [11,12], gas chromatography-mass spectrometry after derivatization [13,14], and liquid chromatography coupled to mass spectrometry (LC-MS/MS), with different sample preparations and chromatographic columns [15][16][17][18][19][20][21][22][23][24]. Due to its high sensitivity and specificity, LC-MS/MS is particularly suitable for measuring low levels of chemicals in urine.…”

Section: Introductionmentioning

confidence: 99%

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Validation of a Liquid Chromatography Coupled to Mass Spectrometry Method for Glyphosate and Aminomethylphosphonic Acid in Urine for Human Biomonitoring Using Combined Hybrid Anion-Exchange and Hydrophilic Interaction Liquid Chromatography

Polledri,

Mercadante,

Fustinoni

2023

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Glyphosate-based herbicides are the most widely used pesticides in the world; however, the toxicity of glyphosate (GlyP) toward humans, especially its carcinogenicity, is controversial. The aim of this work was to validate a rapid assay for measuring GlyP and its metabolite aminomethylphosphonic acid (AMPA) in urine for human biomonitoring. The analytes were purified via solid-phase extraction in the presence of isotopically labeled internal standards. An LC-MS/MS assay was developed using a column with a novel hybrid stationary phase combined with anion exchange and hydrophilic interaction liquid chromatography. Detection and quantification were performed using negative electrospray ionization in a hybrid triple quadrupole/linear ion trap mass spectrometer. The retention times for AMPA and GlyP were 1.44 and 7.24 min, respectively. Calibration curves showed a linear dynamic range of up to 40 µg/L, inter- and intra-run precisions <7.5%, and accuracies within 10% of the theoretical concentrations. The limits of quantification were 0.1 µg/L and 0.5 µg/L for GlyP and AMPA, respectively. The matrix effect bias was controlled using internal standards. Successful participation in external quality assurance exercises strengthens the validity of the method. The assay was applied to the measurement of GlyP and AMPA in the urine of 9 urban residents, 26 rural residents, and 12 agricultural workers; while AMPA was mostly not quantifiable, the median GlyP values were 0.1 and 0.34 µg/L in rural residents and workers, respectively. The assay is useful to assess GlyP and AMPA in human urine following different exposure scenarios.

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Section: Introductionmentioning

confidence: 99%

“…Recently, novel columns with hybrid stationary phases combined with ion exchange and hydrophilic interaction liquid chromatography have become available. They increase the retention times of GlyP without the need for sample derivatization and ensure good chromatographic performances and column duration [24].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Liquid Chromatography Coupled to Mass Spectrometry Method for Glyphosate and Aminomethylphosphonic Acid in Urine for Human Biomonitoring Using Combined Hybrid Anion-Exchange and Hydrophilic Interaction Liquid Chromatography

Polledri,

Mercadante,

Fustinoni

2023

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Analytical methods for determining environmental contaminants of concern in water and wastewater

Kadadou,

Tizani,

Alsafar

et al. 2024

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An analytical method for the determination of glyphosate and aminomethylphosphoric acid using an anionic polar pesticide column and the application in urine and serum from glyphosate poisoning patients

Abstract: An analytical method for the glyphosate (GLY) and aminomethylphosphoric acid (AMPA) in biological fluid samples (serum and urine) from poisoning patients by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established. After...

Cited by 2 publications

References 18 publications

Validation of a Liquid Chromatography Coupled to Mass Spectrometry Method for Glyphosate and Aminomethylphosphonic Acid in Urine for Human Biomonitoring Using Combined Hybrid Anion-Exchange and Hydrophilic Interaction Liquid Chromatography

Validation of a Liquid Chromatography Coupled to Mass Spectrometry Method for Glyphosate and Aminomethylphosphonic Acid in Urine for Human Biomonitoring Using Combined Hybrid Anion-Exchange and Hydrophilic Interaction Liquid Chromatography

Analytical methods for determining environmental contaminants of concern in water and wastewater

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