A simple gas chromatographic identification and determination of 11 CNS stimulants in biological samples. Application on a fatality involving phendimetrazine

Kintz, Pascal; Tracqui, A.; Mangin, Patrice; Lugnier, A.; Chaumont, A. J.

doi:10.1016/0379-0738(89)90142-4

Cited by 18 publications

(2 citation statements)

References 17 publications

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“…The commonly employed methods are high-performance liquid chromatography with fluorescence detection (Bowyer et al, 1995;Farrell and Jeffries, 1983;Foster et al, 1998;Bourque et al, 1994), capillary electrophoresis (Kuroda et al, 1998), immunoassay (Caplan et el., 1987;Cody and Schwarzhoff 1993), GC with nitrogen-phosphorus detection (Cheung et al, 1997;Koide et al, 1998), flame-ionization detection (Kintz et al, 1989), electron-capture detection (Roy et el., 1984;Paetsch et al, 1992), and MS (Valentine et al, 1995;Battu et al, 1998;Marquet et al, 1997;Tsai et al, 1998). The matrices commonly assayed for amphetamine are human whole blood (Sato and Mitsui, 1997;Gjerde et al, 1993), plasma (Pizarro et al, 1999), urine (Fisher and Bourque, 1993), cerebrospinal fluid (Narasimhachari et al, 1979), and hair (Kintz et al, 1989). GC-MS has become most popular in the quantitation of amphetamine in various biological matrices due to the high specificity and sensitivity offered by this technique.…”

Section: Introductionmentioning

confidence: 96%

Determination of amphetamine in dog plasma by gas chromatography with mass selective detection

March

Karnes

McLean

et al. 2001

Biomedical Chromatography

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This paper describes the validation of an analytical method for the determination of amphetamine in beagle dog plasma by gas chromatography coupled to mass spectrometry (GC-MS). d-Amphetamine-d(6) was used as the internal standard. The method consisted of a rapid single-step liquid-liquid extraction and derivatization of amphetamine with 2,2,2-trichloroethyl chloroformate, followed by sensitive GC-MS detection. This is the first report utilizing the combination of trichloroethyl chloroformate as a derivatization reagent and a deuterated amphetamine analog as an IS for the quantification of amphetamine in plasma. The method was validated in terms of specificity, curve fit, precision, accuracy, recovery and stability, and was acceptable according to FDA draft guidelines for validation of bioanalytical methods. The limit of detection was 0.65 ng/mL. The calibration range was 5-150 ng/mL. The validated method was successfully employed for the quantitation of amphetamine in dog plasma samples for pharmacokinetic profiling.

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Section: Introductionmentioning

confidence: 96%

Determination of amphetamine in dog plasma by gas chromatography with mass selective detection

March

Karnes

McLean

et al. 2001

Biomedical Chromatography

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“…A modified version of a commercially available RIA was investigated as a screen for methamphetamine in whole blood and urine (208). Underivatized amphetamine has been identified and quantified in human whole blood by GC-NPD (209) and in lasma and urine by GC-FID (210). Derivatization has also een used to identify and quantify amphetamine and methamphetamine in urine by solid-phase extraction and GC/MS or GC-NPD (211), liquid-liquid extraction and GC/MS (212,213), and capillary GC-FID (214).…”

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confidence: 99%