Forensic science

Brettell, Thomas A.; Saferstein, Richard

doi:10.1021/ac00012a010

Cited by 9 publications

(2 citation statements)

References 374 publications

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“…Many applications that currently use gel electrophoresis to size and separate DNA fragments are foreseen for this rapid and relatively simple technique. They include new, gel-based approaches to DNA sequencing (20,21) and DNA fingerprinting for medical diagnostics and forensic studies (22). We expect additional applications which take advantage of the speed, small sample size requirements, and accuracy of this approach will be developed.…”

Section: Resultsmentioning

confidence: 99%

Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry

Goodwin¹,

Johnson²,

et al. 1993

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Large, fluorescently stained restriction fragments of lambda phage DNA are sized by passing individual fragments through a focused continuous wave laser beam in an ultrasensitive flow cytometer at a rate of 60 fragments per second. The size of the fluorescence burst emitted by each stained DNA fragment, as it passes through the laser beam, is measured in one millisecond. One hundred sixty four seconds of fluorescence burst data allow linear sizing of DNA with an accuracy of better than two percent over a range of 10 to 50 kbp. This corresponds to analyzing less than 1 pg of DNA. Sizing of DNA fragments by this approach is much faster, requires much less DNA, and can potentially analyze large fragments with better resolution and accuracy than with gel-based electrophoresis.

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Section: Resultsmentioning

confidence: 99%

Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry

Goodwin¹,

Johnson²,

et al. 1993

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“…Indeed, the technique will continue to function as long as the mission has power. Autofluorescence biosignatures extracted from spectra and/or images have become a fundamental part of forensic crime scene analysis (Brettell & Saferstein 1991) and biowarfare defence (Kim et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Complete fluorescent fingerprints of extremophilic and photosynthetic microbes

Dartnell

Storrie-Lombardi²,

Ward

2010

International Journal of Astrobiology

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The work reported here represents a study into the total fluorescence exhibited by a broad selection of model, extremophilic and photosynthetic bacterial strains, over a great range of excitation and emission wavelengths from ultraviolet (UV) through visible to near infrared. The aim is to identify distinctive fluorescent features that may serve as detectable biosignatures of remnant microbial life on the Martian surface. A lab-bench fluorescence spectrometer was used to generate an excitation-emission matrix (EEM) for the unpigmented Escherichia coli, radiation-resistant Deinococcus radiodurans, Antarctic Dry Valley isolates Brevundimonas sp. MV.7 and Rhodococcus sp. MV.10, and the cyanobacterium Synechocystis sp. PCC 6803. Detailed EEMs, representing the fluorescence signature of each organism, are presented, and the most significant features suitable for biosignature surveys are identified, including small-molecule cellular metabolites, light-harvesting photosynthetic pigments and extracellular UV-screening compounds. E. coli exhibits the most intense emission from tryptophan, presumably due to the absence of UV-screening pigments that would shield the organism from shortwavelength light-exciting intracellular fluorescence. The efficacy of commonly available laser diodes for exciting cellular fluorescence is treated, along with the most appropriate filter wavelengths for imaging systems. The best combination of available laser diodes and PanCam filters aboard the ExoMars probe is proposed. The possibility of detecting fluorescence excited by solar UV radiation in freshly exposed surface samples by imaging when both sunlit and shadowed, perhaps by the body of the rover itself, is discussed. We also study how these biological fluorophore molecules may be degraded, and thus the potential biosignatures erased, by the high flux of far-ultraviolet light on Mars.

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Analysis (Chemical)

2005

Van Nostrand's Encyclopedia of Chemistry

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The Revolution in Analytical Chemistry—A Perspective Traditional Analytical Chemistry A Gradual Break from Tradition Energy—Matter Interactions in Analytical Instrumentation Targets of Analytical Instrumentation Criteria for Selecting Appropriate Analytical Method

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Forensic science

Cited by 9 publications

References 374 publications

Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry

Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry

Complete fluorescent fingerprints of extremophilic and photosynthetic microbes

Analysis (Chemical)

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