A Simple Fluorescent Aptasensing Platform Based on Graphene Oxide for Dopamine Determination

Teniou, Ahlem; Rhouati, Amina; Catanante, Gaëlle

doi:10.1007/s12010-022-03802-1

Cited by 16 publications

(7 citation statements)

References 67 publications

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“…Teniou et al proposed a fluorescent aptamer sensor based on GO as a quenching agent and FRET principle for the rapid determination of dopamine (DA) [ 108 ]. This sensor showed excellent selectivity and sensitivity.…”

Section: Application Of Go Fluorescence Quenchingmentioning

confidence: 99%

Photoluminescence and Fluorescence Quenching of Graphene Oxide: A Review

Xiao

Zhou

et al. 2022

Nanomaterials

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In recent decades, photoluminescence (PL) material with excellent optical properties has been a hot topic. Graphene oxide (GO) is an excellent candidate for PL material because of its unique optical properties, compared to pure graphene. The existence of an internal band gap in GO can enrich its optical properties significantly. Therefore, GO has been widely applied in many fields such as material science, biomedicine, anti-counterfeiting, and so on. Over the past decade, GO and quantum dots (GOQDs) have attracted the attention of many researchers as luminescence materials, but their luminescence mechanism is still ambiguous, although some theoretical results have been achieved. In addition, GO and GOQDs have fluorescence quenching properties, which can be used in medical imaging and biosensors. In this review, we outline the recent work on the photoluminescence phenomena and quenching process of GO and GOQDs. First, the PL mechanisms of GO are discussed in depth. Second, the fluorescence quenching mechanism and regulation of GO are introduced. Following that, the applications of PL and fluorescence quenching of GO–including biomedicine, electronic devices, material imaging–are addressed. Finally, future development of PL and fluorescence quenching of GO is proposed, and the challenges exploring the optical properties of GO are summarized.

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Section: Application Of Go Fluorescence Quenchingmentioning

confidence: 99%

Photoluminescence and Fluorescence Quenching of Graphene Oxide: A Review

Xiao

Zhou

et al. 2022

Nanomaterials

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“…From our preliminary experiments and literature, the concentration of EDC, ethanolamine (EA), and aptamer were identified as the critical input parameters that significantly influence Hb detection. The concentration range and levels of the selected parameters are shown in Table .…”

Section: Experimental Designmentioning

confidence: 99%

“…This method aims to find a polynomial that fit the experimental data well and determines the optimal conditions based on the variables and their effect on each other. 32 From our preliminary experiments and literature 33 , 34 the concentration of EDC, ethanolamine (EA), and aptamer were identified as the critical input parameters that significantly influence Hb detection. The concentration range and levels of the selected parameters are shown in Table 1.…”

Section: ■ Experimental Designmentioning

confidence: 99%

Colorimetric Detection of Hemoglobin by Aptamer-Based Biosensor

Teniou¹,

Rhouati²,

Madi³

et al. 2023

Ind. Eng. Chem. Res.

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Hemoglobin is an essential protein located in red blood cells. This protein's highly sensitive and selective detection plays a vital role in diagnosing several pathologies, including urinary, renal, and infectious diseases. Therefore, exploring simple and accurate methods for Hb detection is very important. In this work, we have developed an aptasensor based on a label-free colorimetric detection method for hemoglobin detection. The bovine serum albumin (BSA) adsorbed on the polystyrene microplate wells was used to immobilize an amino-modified aptamer specific for Hb. To avoid the labeling step, the peroxidase activity of Hb was explored for a direct generation of the colorimetric signal by catalyzing the peroxidase substrate TMB. The catalytic reaction of TMB occurs through the hemoglobin′s peroxidase activity, resulting in a blue-colored precipitate. Since the optimization steps are expensive, time-consuming, and require a large amount of reagents, response surface methodology based on Box-Behnken Design (RSM-BBD) and artificial neural network coupled with particle swarm optimization (ANN-PSO) were used as optimization methods to develop this colorimetric platform. The performance of RSM-BBD and ANN-PSO was compared, and the results were deeply analyzed. Colorimetric measurements have led to a linear range of 4−10 mg mL −1 and a detection limit of 0.044 mg mL −1 . In the proposed strategy, using the BSA molecule as an immobilization support and RSM-BBD and PSO-ANN as an optimization tool makes this label-free detection platform simpler and inexpensive.

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“…A 57-mer anti-DA DNA aptamer was reported with good affinity ( K d = 150 nM) by Nakatsuka et al in 2018 . After that, various aptasensors or aptasensing method were developed for the determination of DA by the employment of colorimetric, fluorescence, , electrochemical, , and electrogenerated chemiluminescence (electrochemiluminescence, ECL) methods.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrogenerated Chemiluminescence Aptasensing Method for Highly Sensitive Determination of Dopamine via Target-Induced DNA Conformational Change

et al. 2023

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A label-free electrogenerated chemiluminescence (ECL) aptasensing method for highly sensitive determination of dopamine (DA) was developed based on target-induced DNA conformational change. After anti-DA specific aptamer, as molecular recognition element, was hybridized with a capture ss-DNA (complementary with the aptamer), the formed double-strand DNA (ds-DNA) was self-assembled onto the surface of a gold electrode, and then Ru(phen)3 2+, as ECL reagent, was intercalated into ds-DNA to form an ECL biosensing platform. In the presence of DA, DA bound with its aptamer and target-induced DNA conformational change occurred, resulting in the dissociation of ds-DNA, the release of intercalated Ru(phen)3 2+ from the electrode surface, and the decrease of ECL intensity. For comparison, an ECL aptamer-based biosensing method using an ECL reagent-labeled aptamer was also developed for DA assay based on target-induced DNA conformational change. Because of the increase in the amount of ECL reagent into ds-DNA over that of the single-site ECL reagent-labeled aptamer, an obvious increase of ECL intensity was found at the ds-DNA modified electrode over the aptamer modified electrode. DA can be sensitively detected with a lower detection limit of 0.05 nM in the range from 0.1 to 100 nM. With the recognition of the aptamer for DA, DA can be selectively and sensitively detected in artificial cerebrospinal fluid and serum samples without interference from common small molecules. This work demonstrates that the combination of the direct transduction of specific recognition of DA and its aptamer into an ECL signal with Ru(phen)3 2+ intercalated ds-DNA amplification provides a promising strategy for the development of a simple, sensitive, and selective method for DA assay, which is of great importance in neurochemical assays and clinical diagnosis.

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A Simple Fluorescent Aptasensing Platform Based on Graphene Oxide for Dopamine Determination

Cited by 16 publications

References 67 publications

Photoluminescence and Fluorescence Quenching of Graphene Oxide: A Review

Photoluminescence and Fluorescence Quenching of Graphene Oxide: A Review

Colorimetric Detection of Hemoglobin by Aptamer-Based Biosensor

Label-Free Electrogenerated Chemiluminescence Aptasensing Method for Highly Sensitive Determination of Dopamine via Target-Induced DNA Conformational Change

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