A simple and sensitive method for determining reducing sugars in plant tissues. Application to quantify the sugar content in quinoa (Chenopodium quinoa Willd.) seedlings

Prado, Fernando Eduardo; González, Juan Antonio Suarez; Boero, C.; Sampietro, Antonio Rodolfo

doi:10.1002/(sici)1099-1565(199803/04)9:2<58::aid-pca387>3.0.co;2-z

Cited by 35 publications

(12 citation statements)

References 12 publications

(19 reference statements)

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“…The nitrogen analyzer was calibrated against glycine ptoluenesulfonate (Hach, Loveland, Colo.) as a standard. Starch content was quantified by enzymatic conversion to glucose (Prado et al 1998), followed by colorimetric detection of liberated glucose via a modified dinitrosalicyclic acid method (Lindroth et al 2002a). Concentrations of phenolic glycosides (salicortin and tremulacin) were determined by high performance thin-layer chromatography (Lindroth et al 1993a) with standards purified from aspen trees.…”

Section: Foliar Chemistrymentioning

confidence: 99%

Foliar quality influences tree-herbivore-parasitoid interactions: effects of elevated CO 2 , O 3 , and plant genotype

2003

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This study examined the effects of carbon dioxide (CO2)-, ozone (O3)-, and genotype-mediated changes in quaking aspen (Populus tremuloides) chemistry on performance of the forest tent caterpillar (Malacosoma disstria) and its dipteran parasitoid (Compsilura concinnata) at the Aspen Free-Air CO2 Enrichment (FACE) site. Parasitized and non-parasitized forest tent caterpillars were reared on two aspen genotypes under elevated levels of CO2 and O3, alone and in combination. Foliage was collected for determination of the chemical composition of leaves fed upon by forest tent caterpillars during the period of endoparasitoid larval development. Elevated CO2 decreased nitrogen levels but had no effect on concentrations of carbon-based compounds. In contrast, elevated O3 decreased nitrogen and phenolic glycoside levels, but increased concentrations of starch and condensed tannins. Foliar chemistry also differed between aspen genotypes. CO2, O3, genotype, and their interactions altered forest tent caterpillar performance, and differentially so between sexes. In general, enriched CO2 had little effect on forest tent caterpillar performance under ambient O3, but reduced performance (for insects on one aspen genotype) under elevated O3. Conversely, elevated O3 improved forest tent caterpillar performance under ambient, but not elevated, CO2. Parasitoid larval survivorship decreased under elevated O3, depending upon levels of CO2 and aspen genotype. Additionally, larval performance and masses of mature female parasitoids differed between aspen genotypes. These results suggest that host-parasitoid interactions in forest systems may be altered by atmospheric conditions anticipated for the future, and that the degree of change may be influenced by plant genotype.

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Section: Foliar Chemistrymentioning

confidence: 99%

Foliar quality influences tree-herbivore-parasitoid interactions: effects of elevated CO 2 , O 3 , and plant genotype

2003

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“…Starch levels were determined by first separating starch from soluble sugars. Starch was then enzymatically hydrolyzed to glucose using amyloglucosidase (Prado et al 1998). Glucose concentrations were quantified using a modification of the dinitrosalicylic acid method (Lindroth et al 2002a).…”

Section: Phytochemical Analysismentioning

confidence: 99%

Effects of elevated carbon dioxide and ozone on the phytochemistry of aspen and performance of an herbivore

Kopper

Lindroth

2003

Oecologia

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The purpose of this study was to assess the independent and interactive effects of CO(2), O(3), and plant genotype on the foliar quality of a deciduous tree and the performance of a herbivorous insect. Two trembling aspen (Populus tremuloides Michaux) genotypes differing in response to CO(2) and O(3) were grown at the Aspen FACE (Free Air CO(2) Enrichment) site located in northern Wisconsin, USA. Trees were exposed to one of four atmospheric treatments: ambient air (control), elevated carbon dioxide (+CO(2); 560 microl/l), elevated ozone (+O(3); ambient x1.5), and elevated CO(2)+O(3). We measured the effects of CO(2) and O(3) on aspen phytochemistry and on performance of forest tent caterpillar (Malacosoma disstria Hübner) larvae. CO(2) and O(3) treatments influenced foliar quality for both genotypes, with the most notable effects being that elevated CO(2) reduced nitrogen and increased tremulacin levels, whereas elevated O(3) increased early season nitrogen and reduced tremulacin levels, relative to controls. With respect to insects, the +CO(2) treatment had little or no effect on larval performance. Larval performance improved in the +O(3) treatment, but this response was negated by the addition of elevated CO(2) (i.e., +CO(2)+O(3) treatment). We conclude that tent caterpillars will have the greatest impact on aspen under current CO(2) and high O(3) levels, due to increases in insect performance and decreases in tree growth, whereas tent caterpillars will have the least impact on aspen under high CO(2) and low O(3) levels, due to moderate changes in insect performance and increases in tree growth.

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“…Total sugar determination in fruits is also an important parameter to evaluate their ripening stage [5][6][7]. The determination of total reducing sugars is frequently performed by one of a number of colorimetric procedures [2,[4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Most of them involve colorimetric detection based on the oxidation of the carbonyl groups, followed by reaction with a UV-vis absorbing molecule [3,9].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric total reducing sugars assay based on cupric reduction

Başkan

Tütem

Akyüz

et al. 2016

Talanta

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A simple and sensitive method for determining reducing sugars in plant tissues. Application to quantify the sugar content in quinoa (Chenopodium quinoa Willd.) seedlings

Cited by 35 publications

References 12 publications

Foliar quality influences tree-herbivore-parasitoid interactions: effects of elevated CO 2 , O 3 , and plant genotype

Foliar quality influences tree-herbivore-parasitoid interactions: effects of elevated CO 2 , O 3 , and plant genotype

Effects of elevated carbon dioxide and ozone on the phytochemistry of aspen and performance of an herbivore

Spectrophotometric total reducing sugars assay based on cupric reduction

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