A simple and sensitive LC/MS/MS assay for 7-ethyl-10-hydroxycamptothecin (SN-38) in mouse plasma and tissues: application to pharmacokinetic study of liposome entrapped SN-38 (LE-SN38)

A pH-responsive prodrug delivery system of 10-HCPT for controlled release and tumor targeting

“…The clear supernatant was dried under nitrogen at 40°C, reconstituted by 100 µL acetonitrile and centrifuged at 12,000 rpm for 10 min before analysis. 23,37,38 Tissues were homogenized in saline. Samples of 200 µL of tissue homogenate were analyzed using the same processing steps as for the plasma samples.…”

Quantification of CPT13 in rat plasma using LC–MS/MS for a pharmacokinetic study

“…In recent years, several methods, such as high-performance liquid chromatography (HPLC) with fluorescence detection [9][10][11][12] and mass spectrometry [13][14][15] have been reported for the extraction and detection of CPT and its analogues in biological fluid. Here we describe a sensitive LC-MS/MS to investigate the pharmacokinetic profile of CPT13 in rat plasma using CPT as an internal standard (IS).…”

Historical review of sample preparation for chromatographic bioanalysis: pros and cons

“…Our experiences with PPT using ZnSO 4 , (NH4) 2 SO 4 , heat, and trichloroacetic acid to reduce the protein content of plasma before online SPE confirmed that most analytes precipitate with the aggregates leaving a small fraction in the supernatant. The loss of analyte may be the underlying reason that almost all bioanalytical PPT method use water mixable organic solvents to precipitate the proteins [Jayewardene et al, 2001;L Zhang et al, 2003;Yao et al, 2003;D Chang et al, 2005;Khan et al, 2005;Chung et al, 2006;Guo et al, 2006;Honeywell et al, 2007;. There are several reports on using ZnSO 4 , but an organic solvent was also used [Bergqvist et al, 1993;Egge-Jacobsen et al, 2004].…”