A simple and sensitive ELISA of antibodies to pertussis antigens

Kuno-Sakai, Harumi; Kimura, Mikio; Ohta, Kazuyo; Oh, Yasumasa; Kim, Ryuichi; Kobayashi, Takashi; Yamamoto, Eizi; Fujita, Isao

doi:10.1016/0264-410x(92)90377-v

Cited by 16 publications

(6 citation statements)

References 4 publications

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“…Serum samples were tested for antibodies to B. pertussis pertussis toxin (PT) using an enzyme-linked immunosorbent assay method (Wako Chemicals, Tokyo, Japan) [21]. Antibodies against M. pneumoniae were measured with the use of a particle agglutination (PA) test (Serodia-Myco II kit, Fujirebio, Tokyo, Japan), Legionella species by a microagglutination test (detection of L. pneumophila serogroups 1 ~ 6, L. bozemanii , L. dumoffii , L. gormanii , and L. micdadei ), Coxiella burnetii by an indirect immunofluorescence test, and viruses (influenza A and B viruses, adenovirus, respiratory syncytial virus, cytomegalovirus, and parainfluenza virus types 1, 2, and 3) by a complement fixation test.…”

Section: Methodsmentioning

confidence: 99%

Diagnostic value of symptoms and laboratory data for pertussis in adolescent and adult patients

et al. 2013

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BackgroundSeveral symptoms are classically thought to be suggestive of pertussis in children, but the diagnostic value of these symptoms in adolescent and adult patients is unclear. We evaluated the accuracy of the clinical findings for the early presumptive diagnosis of pertussis in adolescent and adult patients. Furthermore, we measured fractional exhaled nitric oxide (FeNO) with regard to whether we could distinguish eosinophilic inflammation of the airway and pertussis. FeNO is not expected to be associated with pertussis.MethodsWe compared 183 cases with laboratory-confirmed pertussis using serology and polymerase chain reaction and 1,132 cases without laboratory-confirmed pertussis.ResultsAmong pertussis patients, paroxysmal cough was common with 90% sensitivity, but the specificity was low (25%). Posttussive vomiting and whoop were less common (sensitivity 25% and 19%, respectively), but both showed greater specificity for pertussis (80% and 86%, respectively). Posttussive gagging was observed with intermediate frequency and provided greater specificity (49% and 77%, respectively). Pertussis cases were most frequent between May and August with a peak in June. The mean FeNO value for the pertussis patients was 18.2 ± 9.2 ppb, which was significantly lower than that in asthma patients (56.9 ± 20.3 ppb, p <0.001). The most useful definition was posttussive vomiting and/or gagging, and a plus normal FeNO value, which had a sensitivity of 72% and a specificity of 70%.ConclusionsClinical symptoms and laboratory data are of limited value in making the diagnosis of pertussis, and it was clinically difficult to differentiate adolescent and adult patients with or without pertussis. However, pertussis should be considered if patients have posttussive vomiting and/or gagging and a normal FeNO concentration.

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Section: Methodsmentioning

confidence: 99%

Diagnostic value of symptoms and laboratory data for pertussis in adolescent and adult patients

et al. 2013

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“…In 1981, six manufacturers started to produce the acellular pertussis vaccine, however, as of September 2002 Chiba Serum Institute discontinued manufacture. Takeda Chemical Industries Ltd 4–8 , Kitasato Institute, and Denka Seiken Co. Ltd. are producing authentic T type acellular pertussis vaccine, which contains at least four components, that is pertussis toxin (PT), filamentous hemagglutinin (FHA), 69 kDa outer membrane protein (69 kDa OMP), and Agglutinogen 2. The Research Foundation for Microbial Diseases of Osaka University (Biken) produces B type vaccine, a three component vaccine that contains PT, FHA and 69 kDA OMP 4 .…”

Section: Methodsmentioning

confidence: 99%

Safety and efficacy of acellular pertussis vaccine in Japan, evaluated by 23 years of its use for routine immunization

Kuno-Sakai¹,

Kimura

2004

Pediatrics International

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“…Positive level of PT and FHA was defined as !10 Japanese EIA unit (J-EU/ml) [34]. Antibodies against Fim2, Fim3, catACT, and cFHA were examined by a fluorescent immunosorbent assay (Flu EIA), which was modified from the conventional EIA method [31].…”

Section: Serological Examinationsmentioning

confidence: 99%

Detection of antibodies against fimbria type 3 (Fim3) is useful diagnostic assay for pertussis

Oguchi¹,

Miyata²,

Kazuyama³

et al. 2015

Journal of Infection and Chemotherapy

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A simple and sensitive ELISA of antibodies to pertussis antigens

Cited by 16 publications

References 4 publications

Diagnostic value of symptoms and laboratory data for pertussis in adolescent and adult patients

Diagnostic value of symptoms and laboratory data for pertussis in adolescent and adult patients

Safety and efficacy of acellular pertussis vaccine in Japan, evaluated by 23 years of its use for routine immunization

Detection of antibodies against fimbria type 3 (Fim3) is useful diagnostic assay for pertussis

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