Detection of antibodies against fimbria type 3 (Fim3) is useful diagnostic assay for pertussis

Oguchi, Kaoru; Miyata, Akiko; Kazuyama, Yukimasa; Noda, Atsuya; Suzuki, Eri; Watanabe, Mineo; Nakayama, Tetsuo

doi:10.1016/j.jiac.2015.05.006

Cited by 5 publications

(4 citation statements)

References 38 publications

(29 reference statements)

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“…The mean anti-PT antibody titer increased from 16-21 EU/ mL at 6-7 years to 22-26 EU/mL at 12-13 years, and 23-36 EU/mL at 18-19 years, without any signs of pertussis-like illness. In this cross-sectional serological study, the peak age-specific incidence of pertussis was 7-12 years, which was confirmed by the detection of pertussis genome in an epidemiological study (13). Taken together, our results and those of previous studies suggest that an additional dose of DTaP should be added to the routine immunization schedule.…”

Section: Discussionsupporting

confidence: 86%

“…However, anti-PT antibodies decreased in a large number of subjects. Subclinical infections were suspected because of a significant increase in anti-PT antibodies, which was supported by data from our previous epidemiological report (13). Positive rates of antibodies against other targets did not decrease; however, titers of antibodies against poliovirus type III decreased in a few subjects.…”

Section: Discussionsupporting

confidence: 65%

“…In Japan, with a long history of DTaP use, the number of reported cases of pertussis has increased in young adolescents and adults since 2002 (11,12). However, recently, the peak age-specific incidence was determined as 7-9 years old (13).…”

Section: Introductionmentioning

confidence: 99%

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Persistence of Antibodies against Diphtheria, Tetanus, Pertussis, and Poliovirus Types I, II, and III Following Immunization with DTaP Combined with Inactivated Wild-Type Polio Vaccine (DTaP-wIPV)

et al. 2019

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A follow-up serological study was conducted involving 47 subjects who received 4 doses of diphtheria and tetanus toxoids, combined with the acellular pertussis vaccine (DTaP) together with Salk-type inactivated polio vaccine (DTaP-wIPV), until 6 years of age. All antibody levels declined more rapidly than expected within 3 years after the completion of primary vaccination with the 4th booster dose, and titers persisted until 6 years of age. The positive rate of the IgG antibody against pertussis toxin (PT) was 31.9% (15/47) at 4 years of age, 41.0% (16/39) at 5 years of age, and 40.5% (15/37) at 6 years of age. A significant increase in anti-PT antibodies was observed in 6 subjects, suggesting subclinical infection. Positive rates of antibodies against other targets did not decrease; however, titers of neutralizing antibodies against poliovirus type III decreased in a few subjects. These data suggest the need for an additional preschool booster immunization using DTaP-wIPV.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 65%

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Persistence of Antibodies against Diphtheria, Tetanus, Pertussis, and Poliovirus Types I, II, and III Following Immunization with DTaP Combined with Inactivated Wild-Type Polio Vaccine (DTaP-wIPV)

et al. 2019

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“…This can probably be explained by the drawbacks of the immunoblot technology used (semiquantitative, visual scoring). The clinical usefulness of Fim3 antibodies in adolescents and adults was demonstrated by Oguchi et al [36]. Although the use of different antigens (native/denaturated) in reported studies cannot be excluded, the present and other studiesdreviewed by van der Zee et al [6]ddid not reveal any added value for pertussis serodiagnosis.…”

Section: Discussionsupporting

confidence: 40%

Assessment of IgA anti-PT and IgG anti-ACT reflex testing to improve Bordetella pertussis serodiagnosis in recently vaccinated subjects

Subissi

Rodeghiero

Martini

et al. 2020

Clinical Microbiology and Infection

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Objectives: Quantifying IgG antibodies to pertussis toxin (PT) is the most specific and sensitive method for the serodiagnosis of a Bordetella pertussis infection. Since PT is a component of acellular pertussis vaccines, anti-PT IgG is also induced by vaccination, precluding pertussis serodiagnosis based exclusively on anti-PT IgG in recently vaccinated subjects. Here, we aim to identify additional B. pertussis-specific serological markers that can discriminate between infection and recent vaccination. Methods: The clinical usefulness of measuring IgA directed to the vaccine antigen PT and IgG directed to non-vaccine antigens (Fim2/3, LPS, ACT, CatACT) was evaluated in nine well characterized subject groups, aged 10e89 years (n ¼ 390). Serum anti-PT IgG levels (>125 IU/mL) served as an indicator for a recent B. pertussis infection. Comparing symptomatic pertussis-infected subjects (n ¼ 140) with recently vaccinated, non-infected subjects (n ¼ 100) revealed the optimal cut-off, accuracy, sensitivity and specificity for each single parameter. Results: For pertussis diagnosis in recently vaccinated subjects, the measurement of anti-PT IgA (cut-off 15 IU/mL) and anti-ACT IgG (cut-off 15 U/mL) resulted in accuracies of 95% (91.5e97.1) and 87.5% (82.7 e91.1), sensitivities of 92.9% (87.4e96.0) and 83.6% (76.5e88.8) and specificities of 98% (93.0e99.4) and 93% (86.3e96.6), respectively. Comparing anti-PT IgA levels between the youngest (10e19 years, n ¼ 38) and oldest (70e89 years, n ¼ 17) age groups revealed an age-dependent increase in antibody levels in pertussis-infected subjects (p < 0.0001). Conclusions: Reflex testing of anti-PT IgA and anti-ACT IgG improves pertussis serodiagnosis in recently vaccinated symptomatic subjects with elevated anti-PT IgG levels. Furthermore, both markers can discriminate between vaccination and recent infection in pertussis serosurveillance studies.

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Vaccine acquired pertussis immunity was weakened at 4 years of age and asymptomatic pertussis infection was suspected based on serological surveillance

Nakayama

Suzuki

Noda

2019

Journal of Infection and Chemotherapy

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Detection of antibodies against fimbria type 3 (Fim3) is useful diagnostic assay for pertussis

Cited by 5 publications

References 38 publications

Persistence of Antibodies against Diphtheria, Tetanus, Pertussis, and Poliovirus Types I, II, and III Following Immunization with DTaP Combined with Inactivated Wild-Type Polio Vaccine (DTaP-wIPV)

Persistence of Antibodies against Diphtheria, Tetanus, Pertussis, and Poliovirus Types I, II, and III Following Immunization with DTaP Combined with Inactivated Wild-Type Polio Vaccine (DTaP-wIPV)

Assessment of IgA anti-PT and IgG anti-ACT reflex testing to improve Bordetella pertussis serodiagnosis in recently vaccinated subjects

Vaccine acquired pertussis immunity was weakened at 4 years of age and asymptomatic pertussis infection was suspected based on serological surveillance

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