A Simple and Robust Protocol for fast RP-HPLC Determination of Salicylates in Foods

Szkop, Michał; Szkop, Urszula; Kęszycka, Paulina Katarzyna; Gajewska, Danuta

doi:10.1007/s12161-016-0621-4

Cited by 16 publications

(14 citation statements)

References 19 publications

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“…The total salicylic acid (free and conjugated forms of SA) contents were measured by the reversed-phase high-performance liquid chromatography (RP-HPLC) with detection of fluorescence according to Szkop et al ( 2017 ) method with minor modifications. Leaves (100 mg) were macerated in a mortar with quartz sand and 0.4 M dipotassium phosphate.…”

Section: Methodsmentioning

confidence: 99%

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Labudda

Tokarz

et al. 2020

Plant Cell Rep

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Key message Defence responses of cyst nematode and/or wheat curl mite infested barley engage the altered reactive oxygen species production, antioxidant machinery, carbon dioxide assimilation and photosynthesis efficiency. Abstract The primary aim of this study was to determine how barley responds to two pests infesting separately or at once; thus barley was inoculated with Heterodera filipjevi (Madzhidov) Stelter (cereal cyst nematode; CCN) and Aceria tosichella Keifer (wheat curl mite; WCM). To verify hypothesis about the involvement of redox metabolism and photosynthesis in barley defence responses, biochemical, photosynthesis efficiency and chlorophyll a fluorescence measurements as well as transmission electron microscopy were implemented. Inoculation with WCM (apart from or with CCN) brought about a significant suppression in the efficiency of electron transport outside photosystem II reaction centres. This limitation was an effect of diminished pool of rapidly reducing plastoquinone and decreased total electron carriers. Infestation with WCM (apart from or with CCN) also significantly restricted the electron transport on the photosystem I acceptor side, therefore produced reactive oxygen species oxidized lipids in cells of WCM and double infested plants and proteins in cells of WCM-infested plants. The level of hydrogen peroxide was significantly decreased in double infested plants because of glutathione–ascorbate cycle involvement. The inhibition of nitrosoglutathione reductase promoted the accumulation of S-nitrosoglutathione increasing antioxidant capacity in cells of double infested plants. Moreover, enhanced arginase activity in WCM-infested plants could stimulate synthesis of polyamines participating in plant antioxidant response. Infestation with WCM (apart from or with CCN) significantly reduced the efficiency of carbon dioxide assimilation by barley leaves, whereas infection only with CCN expanded photosynthesis efficiency. These were accompanied with the ultrastructural changes in chloroplasts during CCN and WCM infestation.

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Section: Methodsmentioning

confidence: 99%

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Labudda

Tokarz

et al. 2020

Plant Cell Rep

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“…To calculate total salicylates intake (free salicylic acid plus bound salicylic acid), we multiplied the daily intake of herbs and spices (g/day) by the salicylates content of 33 condiments item. We have used several databases on salicylates content in food, including the recent own study [9,38,39] as well as other studies [30,[32][33][34][35][36][37]].…”

Section: The Salicylates Intakementioning

confidence: 99%

“…The aims of the study were (i) to assess the intake of herbs and spices at the individual level and (ii) to calculate the dietary salicylates intake from herbs and spices among adult omnivores and vegans. To estimate the intake of salicylates we used several database of salicylates contents in food [30,[32][33][34][35][36][37] including our own studies [9,38,39].…”

Section: Introductionmentioning

confidence: 99%

Intake of Dietary Salicylates from Herbs and Spices among Adult Polish Omnivores and Vegans

et al. 2020

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There is a growing body of evidence that a diet rich in bioactive compounds from herbs and spices has the ability to reduce the risk of chronic diseases. The consumption of herbs and spices is often overlooked in the studies on food intake. However, measurement of dietary intake of these products, as a source of bioactive compounds, including salicylates, has recently gained much significance. The aims of the study were (i) to assess the intake of herbs and spices at the individual level and (ii) to calculate the dietary salicylates intake from herbs and spices among adult omnivores and vegans. The study group consisted of 270 adults aged 19 to 67 years, including 205 women and 65 men. Among all, 208 individuals were following an omnivorous diet while 62 were vegans. A semi-quantitative food frequency questionnaire (FFQ) was designed to assess the habitual intake of 61 fresh and dried herbs and spices during the preceding three months. The five most frequently eaten herbs among omnivores were parsley, garlic, dill, marjoram and basil, while among vegans they were garlic, parsley, ginger, basil and dill. An average intake of all condiments included in the study was 22.4 ± 18.4 g/day and 25.8 ± 25.9 g/day for both omnivores and vegans, respectively (p = 0.007). Estimated medial salicylates intake was significantly higher among vegans (p = 0.000) and reached 5.82 mg/week vs. 3.13 mg/week for omnivores. Our study confirmed that herbs and spices are important sources of salicylates; however, the type of diet influenced their level in the diet. Vegans consume significantly more total salicylates than omnivores.

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“…The total salicylic acid (free and conjugated forms of SA) amounts were quantified using the reversed phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection according to Szkop et al ( 2017 ) with small modifications. Briefly, leaf samples (100 mg) of barley plants were homogenized in a mortar with quartz sand and 1.5 ml 0.4 M K 2 HPO 4 , they were vigorously agitated for 15 min at 70 °C and centrifuged at 16,000×g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Efficient antioxidant defence systems of spring barley in response to stress induced jointly by the cyst nematode parasitism and cadmium exposure

et al. 2020

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Aims This research aimed to establish how Hordeum vulgare responds to abiotic and biotic stress affecting in tandem. Methods Plants were inoculated with Heterodera filipjevi and treated with cadmium (Cd) concentration (5 μM) that can occur in the cultivated soil. To verify the hypothesis about participation of increased antioxidative defence in H. vulgare under stress, biochemical and microscopic methods were implemented. Results The amount of superoxide anions and hydrogen peroxide was diminished in plants that were both nematode-inoculated and cadmium-treated. Superoxide anions were rendered harmless by increased activity of superoxide dismutase, and H2O2 was scavenged via Foyer-Halliwell-Asada pathway. The unique enhanced antioxidant capacity of double stressed plants was also linked with the accumulation of S-nitrosoglutathione as nitrosoglutathione reductase activity was inhibited. Furthermore, stimulated activity of arginase in these plants could promote polyamine synthesis and indirectly enhance non-enzymatic antioxidant mechanism. Results indicate that different antioxidants operating together significantly restricted oxidation of lipids and proteins, thus the integrity of cell membranes and protein functions were maintained. Conclusions The ROS deactivation machinery in barley leaves showed an unusual response during stress induced by H. filipjevi infection and cadmium treatment. Plants could induce a multi-component model of stress response, to detoxify Cd ions and efficiently repair stress damage.

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A Simple and Robust Protocol for fast RP-HPLC Determination of Salicylates in Foods

Cited by 16 publications

References 19 publications

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Intake of Dietary Salicylates from Herbs and Spices among Adult Polish Omnivores and Vegans

Efficient antioxidant defence systems of spring barley in response to stress induced jointly by the cyst nematode parasitism and cadmium exposure

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