A Simple and Rapid PCR-based Method to Isolate Complete Small Macronuclear Minichromosomes from Hypotrich Ciliates: 5S rDNA and S26 Ribosomal Protein Gene of Oxytricha (Sterkiella) nova

Callejas, Sergio; Gutlérrez, Juan Carlos

doi:10.1078/1434-4610-00092

Cited by 3 publications

(2 citation statements)

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“…It is proximately applied to the scrutiny of many organisms since universal primer sets are utilized without any necessity for earlier information about sequences. 3,4 Applications of RAPD involve DNA sequence polymorphisms detection, mapping diverse populations, 5,6 markers isolation associated to different traits or particular targeted intervals 7,8 as well as implementation in parentage analysis and identification of varieties. 9,10 RAPD also allows evolutionary researchers to find the genetic characters of various species that areclosely related.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Genetic Stability of Amaranthus viridis L. Species in Selected Regions of Western Ghats using Random Amplified Polymorphic DNA (RAPD)

Helen,

Bency*

2023

Curr Agri Res Jour

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Random Amplified Polymorphic DNA is cheap and fast molecular technique to identify the genetic resemblances or variations in DNA in various plants. The proposed research work wasintendedto compare the genetic diversification of Amaranthus viridis species in selected regions of Western Ghats in Tamilnadu and Kerala by RAPD analysis.The biometric morphological data among the five A. viridis accessions were compared. Their genomic DNA was isolated and finger prints were obtained using three RAPD markers. The percentage polymorphism, polymorphism information content, effective multiplex ration and resolving power were calculated. UPGMA dendrogram was constructed and their genetic relatedness was compared using Jaccard coefficient.Upon finger printing, 66 bands were counted for the three RAPD primers used,among which 46 bands were polymorphic band numbers from 8 to 21. Percentage of polymorphic bands ranged between 44.44% and 80.77%. EMRvalues for 66 polymorphic loci ranged between 3.56 and 17.01. The UPGMA dendrogram comparing the genomic profiles of the 5 A. viridis accessions using Jaccard coefficient revealed their genetic distances where the maximum Jaccard’s coefficient value observed was 0.96 between AVKATN and AVNEK. Among the 5 selections the peak similarity index (0.9565) was witnessed between AVKATN and AVNEK. The cuurent study revealed that AVKATN and AVNEK had the highest their genetic distances, whereas AVKATN and AVNEK were genetically similar among the 5 A. viridis accessions. Several polymorphic bands which ranged up to 80.77% have proved their genetic variations.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Genetic Stability of Amaranthus viridis L. Species in Selected Regions of Western Ghats using Random Amplified Polymorphic DNA (RAPD)

Helen,

Bency*

2023

Curr Agri Res Jour

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“…Different primers produce RAPDs that have been used extensively as molecular markers. RAPDs also have the advantage that no prior knowledge of the genome is necessary for this application [1][2]. The RAPD markers method has been reported Random Amplified Polymorphic DNA is molecular technique now available, which allows evolutionary biologists to determine the genetic characters of a different variety of closely related animals.…”

Section: Introductionmentioning

confidence: 99%

Random Amplified Polymorphic Dna (Rapd) Based Molecular Characterization of Evolutionary Advancement Amongst Hierarchal Five Vertebrate Species

Rajesh¹,

B²,

N³

et al. 2017

IJAR

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Random Amplified Polymorphic DNA is molecular technique with unique advantage of utility in genetically isolated species without prior genomic information. The prime goal of the present study was to identify molecular characterization and understands the evolutionary advancement among five different vertebrate species. On the basis of the analysis of RAPD profiles amplified by six arbitrary primers that gave the best results in producing species-specific bands (OPG-04, OPG-12, OPG-13, OPG-16, OPG-17 and OPG19), the polymorphic bands were unique in 31.4%, 23.2%, 19.2%,11.3% and 10.8% of rats, avain, gecko, toads and murrels respectively. Based on results, dendrogram constructed for phylogenetic relationship shows wistar rats were most unique and distinct.

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Callejas

Gutiérrez

2003

Molecular Biology Reports

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We have isolated an cDNA after applying a DDRT-PCR analysis on mRNA from mature resting cysts of the ciliate Oxytricha (Sterkiella) nova. From this cDNA fragment the complete macronuclear minichromosome was obtained by using the Mac-End-PCR method. After cloning and sequencing, this cDNA shown certain similarity to HMG-like proteins. The analysis of the inferred amino acid sequence shown that this putative HMG-like protein has one HMG-box interrupted by a intron. The analysis of others characteristics (including a 3D model) confirms that it is a HMGB family protein. It is the first time that a macronuclear gene encoding a putative HMG-box protein is isolated from resting cysts of a stichotrich ciliate. The possible implications of this stored mRNA in the ciliate cryptobiotic stage are discussed.

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A Simple and Rapid PCR-based Method to Isolate Complete Small Macronuclear Minichromosomes from Hypotrich Ciliates: 5S rDNA and S26 Ribosomal Protein Gene of Oxytricha (Sterkiella) nova

Cited by 3 publications

References 31 publications

Evaluation of the Genetic Stability of Amaranthus viridis L. Species in Selected Regions of Western Ghats using Random Amplified Polymorphic DNA (RAPD)

Evaluation of the Genetic Stability of Amaranthus viridis L. Species in Selected Regions of Western Ghats using Random Amplified Polymorphic DNA (RAPD)

Random Amplified Polymorphic Dna (Rapd) Based Molecular Characterization of Evolutionary Advancement Amongst Hierarchal Five Vertebrate Species

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