Random Amplified Polymorphic DNA is molecular technique with unique advantage of utility in genetically isolated species without prior genomic information. The prime goal of the present study was to identify molecular characterization and understands the evolutionary advancement among five different vertebrate species. On the basis of the analysis of RAPD profiles amplified by six arbitrary primers that gave the best results in producing species-specific bands (OPG-04, OPG-12, OPG-13, OPG-16, OPG-17 and OPG19), the polymorphic bands were unique in 31.4%, 23.2%, 19.2%,11.3% and 10.8% of rats, avain, gecko, toads and murrels respectively. Based on results, dendrogram constructed for phylogenetic relationship shows wistar rats were most unique and distinct.
Inhibition of acetylcholinesterase (AChE), the metabolizing enzyme of acetylcholine is evolving as the most important therapeutic target for development of cognitive enhancers. However, AChE activity in brain has not been properly evaluated on the basis of sex. In the present study, AChE expression was investigated in different brain areas of cerebrum and cerebellum in male and female Rattus norvegicus. On comparing male and female genders, increased AChE activity was seen in cerebrum and cerebellum of female Rattus norvegicus. However, no significant change in AChE activity was found between cerebrum and cerebellum within the same male and female. Thus it appears that sex alters AChE activity in different brain regions (G4 isoform) that may vary in male and female. Sequence analysis revealed that highest divergence was found in between male cerebrum and female cerebrum (11.9) and least divergence was found in between male cerebellum and female cerebellum (6.4) with control AChE NM_EF534897
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.