A Simple and Rapid HPLC Method for the Multidetermination of Enrofloxacin, Ciprofloxacin, and Oxytetracyclin in Raw Materials and Veterinary Pharmaceutical Formulations

Anacleto, Sara da Silva; Teixeira, Leila Suleimara; Borges, Marcella Matos Cordeiro; Moraes, Carolina Torres; Borges, Warley de Souza; Pereira, Arnaldo César; Borges, Keyller Bastos

doi:10.2174/1573412913666170613091914

Cited by 8 publications

(3 citation statements)

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“…The analysis was carried out in an isocratic mode at the flow rate of 1.0 ml/min, at 25 °C and detection was carried out at 270 nm. Good linearity with the coefficient of correlation 0.9991 indicated that the proposed method was linear within the range of 0.1-0.6µg/ml compared to prevaling method having linearity with a coefficient of correlation 0.9961 and flow rate of 3.0 ml/min [12]. Accuracy results displayed good reproducibility with % RSD below 2%.…”

Section: Estimationmentioning

confidence: 89%

“…The methods available for Enrofloxacin estimation are thin-layer chromatography (TLC)-fluorescence, capillary electrophoresis-fluorescence, Spectrophotometry [7,8], high-performance liquid chromatography (HPLC)-UV or fluorescence [9][10][11][12][13][14][15][16][17], HPLC-Mass spectrometry (MS) or gas chromatography (GC)-MS [18]. The majority of these methods have been developed to analyze biological samples such as serum or urine.…”

Section: Introductionmentioning

confidence: 99%

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Validation and Application of Rp-HPLC Method for Quantification of Enrofloxacin in Pure and Veterinary Dosage Forms

ASWATHY¹,

MUHAS²,

S.³

et al. 2022

Int J Pharm Pharm Sci

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Objective: The main objective of this study is to develop and validate a simple, new, fast, sensitive, precise and accurate RP-HPLC analytical methods have been established for the estimation of enrofloxacin in bulk and pharmaceutical dosage forms.Methods: The present method was developed and validated on a Waters HPLC system using Phenomenex make Shimadzu C18 column (250 mm × 4.6 mm i.d., 5μm particle size) column was used for the separation. Best results were obtained with the mobile phase composition consisting of Acetonitrile-water (80:20, v/v). The system was regulated at 1.0 ml/min flow rate at 270 nm UV detection. Results: Enrofloxacin was eluted at 3.405 min retention time. The analytical parameters such as accuracy, precision, linearity, LOD, LOQ, ruggedness, and robustness were used for validating the developed method according to ICH guidelines. Linearity was exhibited over the concentration range of 0.1-0.6µg/ml and the Limit of Detection and Quantitation values for Enrofloxacin was 0.001µg/ml and 0.03µg/ml, respectively. The result of the analysis shows that the amount of drugs present in the formulation has a very good correlation with the label claim of the formulation and %RSD will be less than 2 for all the validation parameters. Recoveries studies revealed that results within the specified limits.Conclusion: The developed methods were validated for various parameters as per ICH guidelines. Hence the proposed method was found to be satisfactory and could be used for the routine analysis of enrofloxacin in their marketed formulation.

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Section: Estimationmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Validation and Application of Rp-HPLC Method for Quantification of Enrofloxacin in Pure and Veterinary Dosage Forms

ASWATHY¹,

MUHAS²,

S.³

et al. 2022

Int J Pharm Pharm Sci

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“…The regression equations obtained were y = 0.1302x -0.03378 and y = 0.1336x -0.0088 for TC, and formulation contain-ing TC, respectively. The correlation coefficients (r) of the calibration curves were 0.999 both for TC, and for formulation containing TC, according to RDC 166 (ANVISA, 2017) (17,18,19) minimum acceptable criterion (Figure 3).…”

Section: Linearitymentioning

confidence: 96%

A simple and rapid spectrophotometric method for the determination of trans-chalcone in raw-material and topical formulation

Martinez,

Fukuda,

Silva

et al. 2023

Semin. Cienc. Biol. Saude

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Trans-chalcone (TC) is a flavonoid precursor characterized by a wide spectrum of action, with anti-inflammatory and antioxidant effects. However, no validated methods are available in official compendia for the analysis of this substance. Thus, the aim of this work was to develop and validate a simple, fast, and reproducible spectrophotometric method for the analysis of TC in raw material, and in topical pharmaceutical formulation containing TC. The established conditions were: methanol as extracting solvent, and detection wavelength of 309 nm by UV spectrophotometer. All tests followed the rules of Resolution RDC 166, 2017. The proposed method was selective. Linearity was demonstrated in the concentration range of 1 to 8 μg/mL (r = 0.999). Repeatability and intermediate precision were confirmed by low relative standard deviation values of 1.53% and 2.70% for TC, and of 1.73% and 2.91% for formulation containing TC. Accuracy, evaluated through recovery test, was adequate, with minimum of 98.24% and maximum of 100.23% of recovery. It was observed that the small deliberate modifications done did not interfere with the results, demonstrating the method is robust. The results showed that the method was considered suitable for the intended purpose, inexpensive, easy to apply, selective, linear, precise, accurate, and robust for the determination TC, and pharmaceutical formulation containing TC. Thus, the method developed satisfies the need for an analytical method for the determination of TC, and topical formulation containing TC, being effective, innovative and able to aid in the development of the pharmaceutical field.

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Lateral flow immunoassay based on polydopamine-coated metal–organic framework for the visual detection of enrofloxacin in milk

et al. 2022

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A Simple and Rapid HPLC Method for the Multidetermination of Enrofloxacin, Ciprofloxacin, and Oxytetracyclin in Raw Materials and Veterinary Pharmaceutical Formulations

Cited by 8 publications

References 0 publications

Validation and Application of Rp-HPLC Method for Quantification of Enrofloxacin in Pure and Veterinary Dosage Forms

Validation and Application of Rp-HPLC Method for Quantification of Enrofloxacin in Pure and Veterinary Dosage Forms

A simple and rapid spectrophotometric method for the determination of trans-chalcone in raw-material and topical formulation

Lateral flow immunoassay based on polydopamine-coated metal–organic framework for the visual detection of enrofloxacin in milk

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