A Simple and Rapid Extraction Method of Carbohydrates from Petals or Sepals of Four Floricultural Plants for Determination of Their Content

Norikoshi, Ryo; Imanishi, Hideo; Ichimura, Kazuo

doi:10.2503/jjshs1.77.289

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Preliminary tests were carried out to select a suitable extraction solvent depending on the sample matrix. Several ethanol/water mixtures, according to the methods of Romani et al (7) and Norikishi et al (16), and a 2:1 dichloromethane/methanol mixture, previously used for isolation of carbohydrates from environmental samples (15), were assayed. The last mixture was found to be especially suited for this application due to the efficient isolation of intra-and extracellular sugars from vegetal material without distinction and, thus, was selected as leaching medium.…”

Section: Optimization Of the Determinationmentioning

confidence: 99%

Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

Gómez-González

Ruiz‐Jiménez

Priego-Capote

et al. 2010

J. Agric. Food Chem.

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Qualitative and quantitative profiling of sugars in vegetal materials from Olea europaea cultivars is here reported. Vegetal tissues from olive fruits, leaves, and stems have been characterized by determination of 22 compounds belonging to monosaccharides, disaccharides, trisaccharides, sugar carboxylic acids and alcohols, cyclic polyols, and derived compounds. Sugar isolation was carried out by leaching into a 2:1 dichloromethane/methanol extraction solution under ultrasonic assistance. Multivariate optimization made possible complete isolation of the target fraction in 10 min with an efficiency similar to that provided by a conventional protocol based on 24 h maceration of the vegetal samples. An aliquot of the extract was dried and reconstituted for silylation prior to GC-MS/MS analysis for selective and sensitive identification/quantitation of sugars. Monitoring the target product ions generated after isolation of the precursor ions for each analyte increases the selectivity of the method. The proposed approach is of particular interest for characterization of the sugar fraction in O. europaea, which is of great relevance because of the role of sugars in the metabolism of lipids, proteins, and antioxidants.

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Section: Optimization Of the Determinationmentioning

confidence: 99%

Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

Gómez-González

Ruiz‐Jiménez

Priego-Capote

et al. 2010

J. Agric. Food Chem.

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“…16 out of 18 amino acids were quantified to similar levels regardless of the hydrolysis approach (Table 4). Absence of Trp indicated interference from carbohydrates during hydrolysis [42, 52] which can be removed through prior processing [56–58]. When H 2 O 2 was added to oxidize methionine and cysteine, subsequent hydrolysis with 6 M HCl resulted in halogenation and loss of tyrosine [54].…”

Section: Resultsmentioning

confidence: 99%

Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry

Kambhampati

Evans

et al. 2019

Plant Methods

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Background Methods used to quantify protein from biological samples are often inaccurate with significant variability that requires care to minimize. The errors result from losses during protein preparation and purification and false detection of interfering compounds or elements. Amino acid analysis (AAA) involves a series of chromatographic techniques that can be used to measure protein levels, avoiding some difficulties and providing specific compositional information. However, unstable derivatives, that are toxic and can be costly, incomplete reactions, inadequate chromatographic separations, and the lack of a single hydrolysis method with sufficient recovery of all amino acids hinder precise protein quantitation using AAA. Results In this study, a hydrophilic interaction chromatography based method was used to separate all proteinogenic amino acids, including isobaric compounds leucine and isoleucine, prior to detection by multiple reaction monitoring with LC–MS/MS. Through inclusion of commercially available isotopically labeled ( 13 C, 15 N) amino acids as internal standards we adapted an isotopic dilution strategy for amino acid-based quantification of proteins. Three hydrolysis methods were tested with ubiquitin, bovine serum albumin, (BSA), and a soy protein biological reference material (SRM 3234; NIST) resulting in protein estimates that were 86–103%, 82–94%, and 90–99% accurate for the three protein samples respectively. The methane sulfonic acid hydrolysis approach provided the best recovery of labile amino acids including: cysteine, methionine and tryptophan that are challenging to accurately quantify. Conclusions Accurate determination of protein quantity and amino acid composition in heterogeneous biological samples is non-trivial. Recent advances in chromatographic phases and LC–MS/MS based methods, along with the availability of isotopic standards can minimize difficulties in analysis and improve protein quantitation. A robust method is described for high-throughput protein quantification and amino acid compositional analysis. Since accurate measurement of protein quality and quantity are a requirement for many biological studies that relate to crop improvement or more generally, our understanding of metabolism in living systems, we envision this method will have broad applicability. Electronic supplementary material The online version of this article (10.1186/s13007-019-0430-z) contains supplementary material, which is available to authorized users.

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“…Various polyols are found in ornamental plants of many species; for example, carnation and redbud contain pinitol (Griffin et al, 2004;Norikoshi et al, 2008). Redbud accumulates pinitol under environmental stress and pinitol is the methyl ester of chiro-inositol, a cyclitol (cyclic polyol).…”

Section: Physiological Studies On Various Polyolsmentioning

confidence: 99%

Physiological Roles of Polyols in Horticultural Crops

Kanayama

2009

J. Japan. Soc. Hort. Sci.

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Sucrose is generally considered the primary photosynthate in plants; however, many horticultural crops, including rosaceous fruit trees, synthesize and use polyols (sugar alcohols). This review describes recent progress in physiological research on the metabolism and transport of sorbitol in rosaceous fruit trees, and of mannitol, another common polyol, in horticultural crops. Studies on various polyols other than sorbitol (in rosaceous fruit trees) and mannitol are then described. Polyols play a role not only in the translocation and storage of photosynthates but also in biotic and abiotic responses in many horticultural crops; therefore, this review also provides insights into the effects of polyol metabolism on the biochemical mechanisms of pathogenicity and environmental stress tolerance, including a novel strategy for engineering stress tolerance.

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A Simple and Rapid Extraction Method of Carbohydrates from Petals or Sepals of Four Floricultural Plants for Determination of Their Content

Cited by 6 publications

References 21 publications

Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry

Physiological Roles of Polyols in Horticultural Crops

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