A simple and efficient sequential electrokinetic and hydrodynamic injections in micellar electrokinetic chromatography method for quantification of anticancer drug 5‐fluorouracil and its metabolite in human plasma

Abstract

A simple and sensitive preconcentration strategy using sequential electrokinetic and hydrodynamic injection modes in micellar electrokinetic chromatography with diode array detection was developed and applied for the separation and determination of anticancer agent, 5‐fluorouracil and its metabolite, 5‐fluoro‐2′‐deoxyuridine, in human plasma. Sequential injection modes with increased analyte loading capacity using the anionic pseudo‐stationary phase facilitated collection of the dispersed neutral and charged analytes into narrow zones and improved sensitivity. Several important parameters affecting sample enrichment performance were evaluated and optimized in this study. Under the optimized experimental conditions, 614‐ and 643‐fold and 782‐ and 803‐fold sensitivity improvement were obtained for 5‐fluorouracil and its metabolite when compared with normal hydrodynamic and electrokinetic injection, respectively. The method has good linearity (1–1,000 ng/ml) with acceptable coefficient of determination (r2 > 0.993), low limits of detection (0.11–0.14 ng/ml) and satisfactory analyte relative recovery (97.4–99.7%) with relative standard deviations of 4.6–9.3% (n = 6). Validation results as well as the application to analysis of human plasma samples from cancer patients demonstrate the applicability of the proposed method to clinical studies.

Simultaneous Separation and Analysis of Five Compounds in Cibotium barometz by Micellar Electrokinetic Chromatography with Large-Volume Sample Stacking

“…In micellar electrokinetic chromatography (MEKC), analytes are separated by the differential partition between the pseudo-stationary phase and the surrounding electrolyte [21]. MEKC can also combine different stacking techniques to separate analytes simultaneously according to the properties of the analytes [22], and LVSS is one of the typical representatives [23].…”

Simultaneous Separation and Determination of Nine Active Ingredients in Sanyetangzhiqing by Cyclodextrin-Modified Micellar Electrokinetic Capillary Electrophoresis-Diode Array Detector

“…However, the resolution decreased when SDS concentration was further increased to beyond 40 mM. This might be because the high concentration of SDS could result in strong interaction between micelle and analytes [ 28 ], which reduced the separation efficiency. In consideration of the resolution and peak shape, 40 mM was selected as the optimal concentration for SDS.…”

Simultaneous Separation and Determination of Nine Active Ingredients in Sanyetangzhiqing by Cyclodextrin Modified Micellar Electrokinetic Capillary Electrophoresis-Diode Array Detector

“…However, the resolution decreased when SDS concentration was further increased to beyond 40 mM. Tis might be because the high concentration of SDS could result in strong interaction between micelle and analytes [28], which reduced the separation efciency. In consideration of the resolution and peak shape, 40 mM was selected as the optimal concentration for SDS.…”