A simple and efficient method for detecting avian influenza virus in water samples

Chen, Quanjiao; Chen, Ze

doi:10.1016/j.jviromet.2014.01.013

Cited by 4 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, various methods have been developed for detecting AIV in water. Several studies have reported methods to detect AIV in surface water during field infections of AIV (12)(13)(14)(15), however, most of these studies only showed the efficiency of their methods under laboratory circumstances (16)(17)(18)(19)(20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Detection of Low Pathogenic Avian Influenza Virus Subtype H10N7 in Poultry and Environmental Water Samples During a Clinical Outbreak in Commercial Free-Range Layers, Netherlands 2017

et al. 2020

View full text Add to dashboard Cite

Wild birds are the natural reservoir of the avian influenza virus (AIV) and may transmit AIV to poultry via direct contact or indirectly through the environment. In the Netherlands, a clinically suspected free-range layer flock was reported to the veterinary authorities by the farmer. Increased mortality, a decreased feed intake, and a drop in egg production were observed. Subsequently, an infection with low pathogenic avian influenza virus was detected. This study describes the diagnostic procedures used for detection and subtyping of the virus. In addition to routine diagnostics, the potential of two different environmental diagnostic methods was investigated for detecting AIV in surface water. AIV was first detected using rRT-PCR and isolated from tracheal and cloacal swabs collected from the hens. The virus was subtyped as H10N7. Antibodies against the virus were detected in 28 of the 31 sera tested. An intravenous pathogenicity index (IVPI) experiment was performed, but no clinical signs (IVPI = 0) were observed. Post-mortem examination and histology confirmed the AIV infection. Multiple water samples were collected longitudinally from the free-range area and waterway near the farm. Both environmental diagnostic methods allowed the detection of the H10N7 virus, demonstrating the potential of these methods in detection of AIV. The described methods could be a useful additional procedure for AIV surveillance in water-rich areas with large concentrations of wild birds or in areas around poultry farms. In addition, these methods could be used as a tool to test if the environment or free-range area is virus-free again, at the end of an AIV epidemic.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of Low Pathogenic Avian Influenza Virus Subtype H10N7 in Poultry and Environmental Water Samples During a Clinical Outbreak in Commercial Free-Range Layers, Netherlands 2017

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Both direct and concentrated samples have been used with either isolation or molecular detection. It is more likely that influenza virus will be detected when environmental water samples are concentrated [63,64]. Feces from infected birds contain high levels of virus [9] and do not require concentration.…”

Section: Discussionmentioning

confidence: 99%

Detection of Influenza A viruses at migratory bird stopover sites in Michigan, USA

Lickfett

Clark

Gehring

et al. 2018

Infection Ecology & Epidemiology

View full text Add to dashboard Cite

Introduction: Influenza A viruses have the potential to cause devastating illness in humans and domestic poultry. Wild birds are the natural reservoirs of Influenza A viruses and migratory birds are implicated in their global dissemination. High concentrations of this virus are excreted in the faeces of infected birds and faecal contamination of shared aquatic habitats can lead to indirect transmission among birds via the faecal-oral route. The role of migratory birds in the spread of avian influenza has led to large-scale surveillance efforts of circulating avian influenza viruses through direct sampling of live and dead wild birds. Environmental monitoring of bird habitats using molecular detection methods may provide additional information on the persistence of influenza virus at migratory stopover sites distributed across large spatial scales. Materials and methods: In the current study, faecal and water samples were collected at migratory stopover sites and evaluated for Influenza A by real-time quantitative reverse transcriptase PCR. Results and Discussion: This study found that Influenza A was detected at 53% of the evaluated stopover sites, and 7% and 4.8% of the faecal and water samples, respectively, tested positive for Influenza A virus. Conclusion: Environmental monitoring detected Influenza A at stopover sites used by migratory birds.

show abstract

“…It can be easier to obtain than feces and may contain AIVs from a number of individual birds encompassing a range of species. AIVs may be more likely to accumulate and persist in sediment than water, and sediment does not require concentrating techniques (Khalenkov et al 2008;Zhang et al 2014). Previous studies have demonstrated the presence of AIV in sediment (Lang et al 2008); however, there are several impediments to sediment analysis using traditional diagnostic techniques (e.g., virus isolation and PCR).…”

Section: Introductionmentioning

confidence: 99%

Targeted Resequencing of Wetland Sediment as a Tool for Avian Influenza Virus Surveillance

Himsworth

Duan

Prystajecky

et al. 2020

Journal of Wildlife Diseases

View full text Add to dashboard Cite

show abstract

A simple and efficient method for detecting avian influenza virus in water samples

Cited by 4 publications

References 26 publications

Detection of Low Pathogenic Avian Influenza Virus Subtype H10N7 in Poultry and Environmental Water Samples During a Clinical Outbreak in Commercial Free-Range Layers, Netherlands 2017

Detection of Low Pathogenic Avian Influenza Virus Subtype H10N7 in Poultry and Environmental Water Samples During a Clinical Outbreak in Commercial Free-Range Layers, Netherlands 2017

Detection of Influenza A viruses at migratory bird stopover sites in Michigan, USA

Targeted Resequencing of Wetland Sediment as a Tool for Avian Influenza Virus Surveillance

Contact Info

Product

Resources

About