A Simple and Efficient Method for Somatic Embryogenesis and Plant Regeneration from Leaves of Chrysanthemum [Dendranthema*grandiflorum (Ramat.) Kitamura]

Shinoyama, Harue; Nomura, Yukio; Tsuchiya, Takao; Kazuma, Toshiharu

doi:10.5511/plantbiotechnology.21.25

Cited by 20 publications

(25 citation statements)

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“…Combinations of 2,4-D and the cytokinins (BA, or KN) have been reported for somatic embryogenesis of Chrysanthemum (May and Trigiano 1991;Pavingerova et al 1994;Shinoyama et al 2004;Mandal and Datta 2005). However, TDZ has not been reported so far.…”

Section: Resultsmentioning

confidence: 95%

“…The combinations higher or lower concentrations of the optimal concentration showed distinctly inhibition of number of somatic embryos per explant. In addition, combination of 2 mg/L 2,4-D and 1 mg/L KN recommended by Shinoyama et al (2004) in this study could induce only 8 somatic embryos per explant. Similarly, Shinoyama et al (2004) reported combination of 2 mg/L 2,4-D and 2 mg/L KN that was observed as optimal concentration in this study gave only 6 somatic embryos per explant.…”

Section: Resultsmentioning

confidence: 99%

“…Studies were being intensively made to develop a protocol for somatic embryogenesis. The process can be induced in tissue cultures of Chrysanthemum either directly from the epidermal cells of explants (Mandal and Datta 2005) or indirectly via intervening callus (Shinoyama et al 2004). In this study, somatic embryogenesis was induced directly from the leaf segment.…”

Section: Resultsmentioning

confidence: 99%

“…Among the cytokinins used, KN was found to be the best for number of somatic embryos per explant followed by BA and TDZ. Shinoyama et al (2004) mentioned the KN was found to be better responsive for somatic embryogenesis than BA. However, they reported that the best results of somatic embryogenesis was obtained on the medium containing 2 mg/L 2,4-D and 1 mg/L KN.…”

Section: Resultsmentioning

confidence: 99%

“…Mandal and Datta (2005) also reported direct somatic embryogenesis from ray florets of different Chrysanthemum cultivars, however, embryogenic response (30 %) with number of somatic embryos per responding explant (15.8 ± 1.2) was the best. To date, the number of somatic embryos per explant (21.3) reported by Shinoyama et al (2004) was the highest in Chrysanthemum. Furthermore, there is no information about secondary somatic embryogenesis in Chrysanthemum.…”

Section: Introductionmentioning

confidence: 99%

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Primary and secondary somatic embryogenesis in Chrysanthemum cv. Euro

Naing

Kim

Yun

et al. 2012

Plant Cell Tiss Organ Cult

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Somatic embryogenesis was induced from in vivo grown leaf explants of Chrysanthemum cv. Euro incubated on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 2.0 mg/L Kinetin, yielding the highest mean number of embryos (42 ± 5.97) per explant after 5 weeks of culture. We evaluated the effects of basal medium, various concentrations of sucrose, and timentin on the proliferation of secondary somatic embryos. MS medium was observed to be the more effective in promoting the proliferation of somatic embryos than half-strength Murashige and Skoog (1/2MS). In addition, timentin was also more efficient in induction of secondary embryogenesis than sucrose. Whole plantlets were obtained by culturing of secondary embryos on hormone-free MS medium and successfully acclimated in the green house.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%