A simple and efficient method for the isolation of differentially expressed genes 1 1Edited by J. Karn

Mahadeva, Harin; Mp, Starkey; Fn, Sheikh; Cr, Mundy; Nj, Samani

doi:10.1006/jmbi.1998.2292

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…This produces a subset of 10-60 bands of amplified cDNAs (the profile or index), allowing a comparison between two mRNA populations. The falsepositive rate using this technique is said to be greatly reduced compared to the conventional DD (Mahadeva et al 1998).…”

Section: Dd-based Techniquesmentioning

confidence: 99%

Large-scale gene expression studies of the endometrium: what have we learnt?

Sherwin¹,

Catalano²,

Sharkey³

2006

Reproduction

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The endometrium is a dynamic tissue that undergoes coordinated changes under the influence of steroid hormones. This results in proliferation and differentiation culminating in a receptive state, followed by menstruation and endometrial repair. These functions involve complex interactions between the epithelium, stroma and leucocytes in the endometrium. Understanding the underlying causes of endometrial disorders, such as infertility, endometriosis and heavy menstrual bleeding, therefore represents a considerable challenge. Recently developed techniques, such as differential display and DNA microarrays permit the abundance of thousands of mRNA transcripts within cells or tissues to be measured simultaneously. This provides a new approach to understanding the complex interactions that underlie both healthy and disease states. Responses of the endometrium to hormones or drugs can be studied and the response of the system as an integrated whole can be assessed. Comparisons of endometrium from healthy women and those with endometrial dysfunction have advanced our understanding of key areas of endometrial physiology, including infertility, receptivity, endometriosis and cancer. Using this approach, novel genes controlling specific endometrial functions like receptivity have been identified for functional testing. This paper will review the impact of these techniques for transcript profiling on our understanding of selected areas of endometrial biology and discuss the potential applications in future.

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Section: Dd-based Techniquesmentioning

confidence: 99%

Large-scale gene expression studies of the endometrium: what have we learnt?

Sherwin¹,

Catalano²,

Sharkey³

2006

Reproduction

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“…10 Differential display is a less laborious and more widely used approach, 11 and has yielded promising results, particularly with an adapted indexing based differential display PCR (DD-PCR) technique. 12 Standard differential display techniques involve conversion of RNA to cDNA, which is then split into a series of reactions involving a specific reverse primer and a random forward primer. Amplification of only a limited subset of the RNA present produces a specific RNA ladder for a given primer combination and tissue.…”

Section: Differential Displaymentioning

confidence: 99%

Functional genomics and proteomics: application in neurosciences

Wilson

2004

Journal of Neurology, Neurosurgery & Psychiatry

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“…This was performed as described previously [11]. Briefly, double‐stranded cDNA produced in parallel from 27 μg of pooled ( n =10 animals per group) total LV RNA from 4 h sham and banded groups, was digested with the class IIS enzyme Bbv I before ligation to pools of tagged adapters with sequence‐specific four‐base overhangs of all possible sequences.…”

Section: Methodsmentioning

confidence: 99%

“…Recent advances in molecular techniques have led to the evolution of expression profiling methods such as differential display [7], serial analysis of gene expression [8], molecular indexing [9] and cDNA array screening [10], enabling a more comprehensive assessment of gene expression in disease states. We have developed an efficient method of expression profiling using the principle of molecular indexing [11]. In the present study, we have applied molecular indexing to study the early stages of pressure‐induced LVH in a rat model of aortic banding, in order to identify novel regulatory molecules involved in the initial signalling that leads to the development of LVH.…”

Section: Introductionmentioning

confidence: 99%

ms1, a novel stress‐responsive, muscle‐specific gene that is up‐regulated in the early stages of pressure overload‐induced left ventricular hypertrophy

et al. 2002

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We have identified and characterised a cDNA encoding a novel gene, designated myocyte stress 1 (ms1), that is up-regulated within 1 h in the left ventricle following the application of pressure overload by aortic banding in the rat. The deduced ms1 protein of 317 amino acids contains several putative functional motifs, including a region that is evolutionarily conserved. Distribution analysis indicates that rat ms1 mRNA expression is predominantly expressed in striated muscle and progressively increases in the left ventricle from embryo to adulthood. These findings suggest that ms1 may be important in striated muscle biology and the development of pressure-induced left ventricular hypertrophy. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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A simple and efficient method for the isolation of differentially expressed genes 1 1Edited by J. Karn

Cited by 14 publications

References 27 publications

Large-scale gene expression studies of the endometrium: what have we learnt?

Large-scale gene expression studies of the endometrium: what have we learnt?

Functional genomics and proteomics: application in neurosciences

ms1, a novel stress‐responsive, muscle‐specific gene that is up‐regulated in the early stages of pressure overload‐induced left ventricular hypertrophy

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