A Simple and Effective Method for Producing Nonrandom Peptide Libraries Using Cotton as a Carrier in Continuous Flow Peptide Synthesizers

Abstract

A method has been developed for generating nonrandom peptide libraries on cotton. Disks of cotton fabric were chemically modified to enable peptide synthesis. Incorporation of a 6-aminocaproic acid residue handle on the cellulose turned out to be advantageous. Disks were labeled with silver ink, stacked one on top of another in a continuous flow peptide synthesizer column, and simultaneously subjected to automated synthesis procedures. Depending on the sequences to be synthesized, the automatic synthesis procedure was stopped, and the disks were removed from the column, sorted, and reapplied to subsequent synthesis steps. In this way, individual peptides could be easily prepared in milligram quantities on each of the cotton disks.

Evaluation of Citrus aurantifolia peel and leaves extracts for their chemical composition, antioxidant and anti‐cholinesterase activities

“…Surprisingly, even well‐established methods give rise to new exciting developments. Recently, Wikberg8 et al reported on the synthesis of a library of hexapeptides by means of continuous‐flow methods. They employed common peptide synthesizers and varied their use according to the original approach of Frank and Doering 9.…”

Cotton functionalized with peptides: characterization and synthetic methods

“…Usually, cellulose derivatization involves the hydroxyl groups of cotton on which esters and ethers (ethyl cellulose) can be prepared. This derivatization, in turn, has allowed the use of cotton as a substrate for the ‘solid phase’ synthesis of peptides (SPPS) and for combinatorial syntheses .…”

Continuous Flow Techniques in Organic Synthesis

“…[7] Surprisingly, even well-established methods give rise to new exciting developments. Recently, Wikberg [8] et al reported on the synthesis of a library of hexapeptides by means of continuous-flow methods. They employed common peptide synthesizers and varied their use according to the original approach of Frank and Doering.…”