2017
DOI: 10.1021/acs.analchem.7b03823
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A Simple and Convenient Aptasensor for Protein Using an Electronic Balance as a Readout

Abstract: The electronic balance, one of the most common pieces of equipment in the laboratory, is normally used to directly measure the weight of a target with high accuracy. However, little attention has been paid to the extension of its applications. In this study, an electronic balance was used as a readout to develop a novel aptasensor for protein quantification for the first time. Thrombin was selected as a model target, and its two aptamers recognizing different sites of the protein were used (one aptamer was imm… Show more

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Cited by 56 publications
(35 citation statements)
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“…Inspired by the previous POCT strategies based on different controlled release systems coupling with barometer, glucometer, and hand-held pH meter, we believe that this proposed system can be applied to build an effective signal conversion and amplification strategy to accelerate the development of a temperature and thermometer-based assay platform. Interestingly, it is notable that the increased pressure in an unsealed vessel, as a controlled release system, can be used for weighing water and developing an electronic balance-based sensing system. According to this, we also found that our barometric system can lead to an obvious water overflowing result from the drainage device that connected with the O 2 -generation vessel, due to the increased pressure of the CoOOH nanoflakes mediated H 2 O 2 decomposition process. As a result, the overflowed H 2 O can be collected by a tube, and its amount may be easily measured by an electronic balance.…”
Section: Resultsmentioning
confidence: 63%
“…Inspired by the previous POCT strategies based on different controlled release systems coupling with barometer, glucometer, and hand-held pH meter, we believe that this proposed system can be applied to build an effective signal conversion and amplification strategy to accelerate the development of a temperature and thermometer-based assay platform. Interestingly, it is notable that the increased pressure in an unsealed vessel, as a controlled release system, can be used for weighing water and developing an electronic balance-based sensing system. According to this, we also found that our barometric system can lead to an obvious water overflowing result from the drainage device that connected with the O 2 -generation vessel, due to the increased pressure of the CoOOH nanoflakes mediated H 2 O 2 decomposition process. As a result, the overflowed H 2 O can be collected by a tube, and its amount may be easily measured by an electronic balance.…”
Section: Resultsmentioning
confidence: 63%
“…As high as 18°C temperature change can be readout by thermometer and the detection limit of 0.28 nM was achieved without amplification. Besides, other handheld devices such as electronic balance [368], digital multimeter [369], uric acid meter [370], could also be used for nucleic acid analysis with appropriate signal transduction and signal amplification mechanism. The use of these handheld devices that are widely applied in daily life, opens a new door for simple, cost-effective, user-friendly and portable POC testing of nucleic acids with high specificity and sensitivity and without external bulky instrumentation.…”
Section: Handheld Devicementioning
confidence: 99%
“…One of the most crucial characteristics of conducting polymers is their ability to reversibly alternate between the conducting and insulating electronic states by dopingundoping of ions [15][16][17][18][19][20]. Anions were shown to be the dopants in polyaniline films [21].…”
Section: Introductionmentioning
confidence: 99%