A simple agar plate preparation for effective transfer of Ureaplasma colonies onto nitrocellulose membranes for colony immunoblotting

Zimmerman, Carl-Ulrich R.; Stiedl, Thomas; Spergser, Joachim; Rosengarten, Renate

doi:10.1016/j.mimet.2014.06.013

Cited by 2 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these studies, MBA-negative Ureaplasma isolates were detected following two to three serial passages in culture medium containing MBA-specific antibodies (59,251). More recently, phase variation of the MBA occurred in the absence of any selective (antibody) pressures (255), indicating that this antigen is capable of rapid phase variation. Zimmerman et al (254) hypothesized that the expression of the mba gene (locus UU375) is alternated with expression of an adjacent locus (UU376), which encodes a Ureaplasma-specific conserved hypothetical protein.…”

Section: Multiple-banded Antigen (Mba)mentioning

confidence: 99%

The Human Ureaplasma Species as Causative Agents of Chorioamnionitis

et al. 2017

View full text Add to dashboard Cite

SUMMARY The human Ureaplasma species are the most frequently isolated microorganisms from the amniotic fluid and placentae of women who deliver preterm and are also associated with spontaneous abortions or miscarriages, neonatal respiratory diseases, and chorioamnionitis. Despite the fact that these microorganisms have been habitually found within placentae of pregnancies with chorioamnionitis, the role of Ureaplasma species as a causative agent has not been satisfactorily explained. There is also controversy surrounding their role in disease, particularly as not all women infected with Ureaplasma spp. develop chorioamnionitis. In this review, we provide evidence that Ureaplasma spp. are associated with diseases of pregnancy and discuss recent findings which demonstrate that Ureaplasma spp. are associated with chorioamnionitis, regardless of gestational age at the time of delivery. Here, we also discuss the proposed major virulence factors of Ureaplasma spp., with a focus on the multiple-banded antigen (MBA), which may facilitate modulation/alteration of the host immune response and potentially explain why only subpopulations of infected women experience adverse pregnancy outcomes. The information presented within this review confirms that Ureaplasma spp. are not simply “innocent bystanders” in disease and highlights that these microorganisms are an often underestimated pathogen of pregnancy.

show abstract

Section: Multiple-banded Antigen (Mba)mentioning

confidence: 99%

The Human Ureaplasma Species as Causative Agents of Chorioamnionitis

et al. 2017

View full text Add to dashboard Cite

show abstract

Ureaplasma

Dando¹,

Sweeney²,

Knox³

2019

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

U.re.a.plas'ma. N.L. fem. n. urea urea; Gr. neut. n. plasma anything formed or molded, image, figure; N.L. neut. n. Ureaplasma urea form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Ureaplasma Bacteria in the genus Ureaplasma , pleomorphic, small (100–800 nm in diameter), primarily coccoid cells, devoid of a cell wall, surrounded by a trilaminar‐membrane structure. Coccobacillary and filamentous forms also occur. These bacteria form exceptionally tiny (<0.2 mm) colonies on solid media. Similar to the closely related genus Mycoplasma , ureaplasmas have small (0.75–1.01 Mb) A+T‐rich genomes, use the codon UGA to encode tryptophan, and are nutritionally fastidious. Unlike mycoplasmas they hydrolyze urea rather than glucose or arginine for ATP synthesis. DNA–DNA hybridizations, serotyping of phase‐variable surface‐exposed lipoproteins [the multiple‐banded antigen (MBA)], PCR, and sequencing of the multiple‐banded antigen gene ( mba ), 16S rRNA, and urease genes have defined the species and serovars for this genus. In nature, all species are commensals and/or opportunistic pathogens of vertebrate hosts. Based on the most recent comparative genome analyses, a consensus is emerging that strains associated with humans, the species Ureaplasma urealyticum and Ureaplasma parvum , undergo extensive horizontal gene transfer and may exist collectively as quasi‐species rather than as stable serovars in their native environment. DNA G + C content (mol%) : 25.4–31.9 (complete genome sequences). Type species : Ureaplasma urealyticum Shepard et al. 1974 AL emend. Robertson et al. 2002.

show abstract

A simple agar plate preparation for effective transfer of Ureaplasma colonies onto nitrocellulose membranes for colony immunoblotting

Cited by 2 publications

References 16 publications

The Human Ureaplasma Species as Causative Agents of Chorioamnionitis

The Human Ureaplasma Species as Causative Agents of Chorioamnionitis

Ureaplasma

Contact Info

Product

Resources

About