A simple 3D cryogel co-culture system used to study the role of CAFs in EMT of MDA-MB-231 cells

Zhang, Ge; Song, Xiaoping; Mei, Jie; Ye, Genlan; Wang, Leyu; Yu, Lei; Xing, Malcolm; Qiu, Xiaozhong

doi:10.1039/c6ra28721b

Cited by 13 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After that, they used the co-culture human breast cancer system to construct a xenograft tumor mouse model for in vivo studies. The results showed that carcinoma-associated fibroblasts could also promote deterioration and metastasis of human breast cancer cells in a xenograft tumor [70].…”

Section: Composite Cryogels As Versatile Tools For Biomedical Applmentioning

confidence: 99%

See 1 more Smart Citation

Supermacroporous Composite Cryogels in Biomedical Applications

Saylan

Denizli

2019

Gels

View full text Add to dashboard Cite

Supermacroporous gels, called cryogels, are unique scaffolds that can be prepared by polymerization of monomer solution under sub-zero temperatures. They are widely used in many applications and have significant potential biomaterials, especially for biomedical applications due to their inherent interconnected supermacroporous structures and easy formation of composite polymers in comparison to other porous polymer synthesis techniques. This review highlights the fundamentals of supermacroporous cryogels and composite cryogels, and then comprehensively summarizes recent studies in preparation, functionalization, and utilization with mechanical, biological and physicochemical features, according to the biomedical applications. Furthermore, conclusions and outlooks are discussed for the use of these promising and durable supermacroporous composite cryogels.

show abstract

Section: Composite Cryogels As Versatile Tools For Biomedical Applmentioning

confidence: 99%

“…The co-culture system for the role of carcinoma-associated fibroblasts in the epithelial–mesenchymal transition process of human breast cancer cells in vitro and in vivo. Republished with permission from [70]. Copyright 2017 the Royal Society of Chemistry.…”

Section: Figurementioning

confidence: 99%

Supermacroporous Composite Cryogels in Biomedical Applications

Saylan

Denizli

2019

Gels

View full text Add to dashboard Cite

show abstract

“…Cryogels formed from PEG-heparin have been reported by our group (Bray et al, 2018), where they were used to create a bone microenvironment using mineralized primary human osteoblasts for co-culture with breast cancer cells lines. Another study utilized a gelatin-based cryogel modified with methacrylate groups (GelMA) (Zhang et al, 2017). The authors used the cryogels to create a tumor stromal microenvironment using CAFs derived from breast cancer patients, showing increased cancer cell migration compared to their mono-cultures.…”

Section: Engineering Patient-specific Tumor Microenvironment Modelsmentioning

confidence: 99%

Addressing Patient Specificity in the Engineering of Tumor Models

Bray

Hutmacher

2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Cancer treatment is challenged by the heterogeneous nature of cancer, where prognosis depends on tumor type and disease stage, as well as previous treatments. Optimal patient stratification is critical for the development and validation of effective treatments, yet pre-clinical model systems are lacking in the delivery of effective individualized platforms that reflect distinct patient-specific clinical situations. Advances in cancer cell biology, biofabrication, and microengineering technologies have led to the development of more complex in vitro three-dimensional (3D) models to act as drug testing platforms and to elucidate novel cancer mechanisms. Mostly, these strategies have enabled researchers to account for the tumor microenvironment context including tumor-stroma interactions, a key factor of heterogeneity that affects both progression and therapeutic resistance. This is aided by state-of-the-art biomaterials and tissue engineering technologies, coupled with reproducible and high-throughput platforms that enable modeling of relevant physical and chemical factors. Yet, the translation of these models and technologies has been impaired by neglecting to incorporate patient-derived cells or tissues, and largely focusing on immortalized cell lines instead, contributing to drug failure rates. While this is a necessary step to establish and validate new models, a paradigm shift is needed to enable the systematic inclusion of patient-derived materials in the design and use of such models. In this review, we first present an overview of the components responsible for heterogeneity in different tumor microenvironments. Next, we introduce the state-of-the-art of current in vitro 3D cancer models employing patient-derived materials in traditional scaffold-free approaches, followed by novel bioengineered scaffold-based approaches, and further supported by dynamic systems such as bioreactors, microfluidics, and tumor-on-a-chip devices. We critically discuss the challenges and clinical prospects of models that have succeeded in providing clinical relevance and impact, and present emerging concepts of novel cancer model systems that are addressing patient specificity, the next frontier to be tackled by the field.

show abstract

“…Along with their use as scaffolds for tissue regeneration, 18,[20][21][22] cryogels are also being increasingly explored to develop 3D functional tumor models in vitro. [23][24][25][26] Since cancer cell growth is modulated by the stiffness of the surrounding matrix, with cancerous tissues being generally stiffer than non-malignant tissues, 23,27 cryogels may indeed be useful to mimic the strength and the elasticity of tumors, thus allowing to analyze the biomechanical cues involved in cell-matrix interactions and pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…), which suffer from large batch-to-batch variability and need particular handling care, thus making experimental reproducibility harder to achieve. In this scenario, PEG-based hydrogels 32,[37][38][39][40][41][42][43] and cryogels 23,24,26 appear as powerful alternatives to conventional organotypic culture models for the production of reproducible tumor tissue equivalents.…”

Section: Introductionmentioning

confidence: 99%

Semi‐interpenetrating polymer network cryogels based on poly(ethylene glycol) diacrylate and collagen as potential off‐the‐shelf platforms for cancer cell research

et al. 2021

View full text Add to dashboard Cite

In the present work, we investigated the potential of novel semi-interpenetrating polymer network (semi-IPN) cryogels, obtained through ultraviolet exposure of aqueous mixtures of poly(ethylene glycol) diacrylate and type I collagen, as tunable offthe-shelf platforms for 3D cancer cell research. We synthesized semi-IPN cryogels with variable collagen amounts (0.1% and 1% w/v) and assessed the effect of collagen on key cryogel properties for cell culture, for example, porosity, degradation rate and mechanical stiffness. Then, we investigated the ability of the cryogels to sustain the long-term growth of two pancreatic ductal adenocarcinoma (PDAC) cell populations, the parenchymal Panc1 cells and their derived cancer stem cells. Results revealed that both cell lines efficiently infiltrated, attached and expanded in the cryogels over a period of 14 days. However, only when grown in the cryogels with the highest collagen concentration, both cell lines reproduced their characteristic growth pattern previously observed in collagen-enriched organotypic cultures, biomimetic of the highly fibrotic PDAC stroma. Cellular preembedding in Matrigel, that is, the classical approach to develop/grow organoids, interfered with an efficient intrascaffold migration and growth. Although preliminary, these findings highlight the potential of the proposed cryogels as reproducible and tunable cancer cell research platforms.

show abstract

A simple 3D cryogel co-culture system used to study the role of CAFs in EMT of MDA-MB-231 cells

Abstract: Development of a 3D co-culture system for the study of the role of CAFs in the EMT process of MDA-MB-231 cells in vitro and in vivo.

Cited by 13 publications

References 38 publications

Supermacroporous Composite Cryogels in Biomedical Applications

Supermacroporous Composite Cryogels in Biomedical Applications

Addressing Patient Specificity in the Engineering of Tumor Models

Semi‐interpenetrating polymer network cryogels based on poly(ethylene glycol) diacrylate and collagen as potential off‐the‐shelf platforms for cancer cell research

Contact Info

Product

Resources

About