A significantly non-toxic novel Cobalt(III) Schiff base complex induces apoptosis via G2-M cell cycle arrest in human breast cancer cell line MCF-7

Dasgupta, Sanchari; Kar, Kanisha; Barua, Atish; Ghosh, Diya; Kabi, Bikash; Dewan, Koushik; Chandra, Arpita

doi:10.1016/j.lfs.2022.120963

Cited by 15 publications

(2 citation statements)

References 51 publications

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“…62 The literature has reported that cobalt complexes exhibit good cytotoxicity against cancerous cell lines such as MCF-7 and low toxicity towards normal cell lines up to 100 μM. 25,63,64 The resulting selective cytotoxicity of complex 1 could be attributed to the favorable redox activities of cobalt under physiological conditions, thus enabling the facile delivery of bioactive imidazole and Boc- l -valine ligands at the site of acidic cancer cells which could ultimately lead to the inhibition of cell proliferation and colony formation of MCF-7 and MDA-MB-231 cells (Fig. 14).…”

Section: Resultsmentioning

confidence: 99%

Revelation of potential bioactive water-soluble Boc-l-valine and imidazole appended metal complexes {M = Co(ii), Cu(ii) & Zn(ii)}: synthesis, characterization, ct-DNA binding, pBR322 cleavage, SOD mimetic, and cytotoxicity studies

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Revelation of potential bioactive water-soluble Boc-l-valine and imidazole appended metal complexes {M = Co(ii), Cu(ii) & Zn(ii)}: synthesis, characterization, ct-DNA binding, pBR322 cleavage, SOD mimetic, and cytotoxicity studies

et al. 2023

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“…Histopathology and hematoxylin-eosin (H&E) staining of liver and kidney tissue was done as per protocol suggested by Dasgupta et al , 2022 [56]. For the experimental purpose, euthanasia was done as per CPCSEA guidelines.…”

Section: S-rrna Sequencingmentioning

confidence: 99%

Metallo-protease Peptidase M84 fromBacillus altitudinisinduces ROS dependent apoptosis in ovarian cancer cells by targeting PAR-1

Nag,

Ray,

Tapader

et al. 2023

Preprint

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In pursuit of isolating novel anticancer proteases from environmental microbial isolates, we have purified and identified an extracellular metallo-protease from Bacillus altitudinis named Peptidase M84. This protease selectively triggered apoptosis in human ovarian adenocarcinoma cells (PA-1, SKOV3) and mouse ovarian carcinoma cells (ID8), in addition to exhibiting no significant effect on normal human epithelial ovarian cell (IOSE) and mouse peritoneal macrophage (PEMΦ) cell viabilities. Protease activated receptor-1 (PAR-1); a GPCR which is reported to be overexpressed in ovarian cancer cells was identified as a novel target of Peptidase M84. We observed that Peptidase M84 induced PAR-1 overexpression along with activating its downstream signalling effectors NFκB and MAPK to promote excessive reactive oxygen species (ROS) generation in ovarian cancer cells. This disrupted mitochondrial membrane potential, allowed cytosolic release of mitochondrial cytochrome c, increased the Bax (pro-apoptotic) to Bcl-2 (anti-apoptotic) ratio and promoted DNA damage to evoke apoptotic death of the ovarian cancer cells. Peptidase M84 also reduced nuclear ki-67 expression in these malignant cells to render an anti-proliferative role. In in vivo set-up, weekly intraperitoneal administration of Peptidase M84 (12 ug/kg body-weight) in the ID8 mice model significantly diminished ascitic fluid accumulation through induction of oxidative stress, increasing murine survival rates by 60%. Collectively, our in vitro and in vivo findings suggested that Peptidase M84 triggered PAR-1 mediated oxidative stress to act as an apoptosis inducer in ovarian cancer cells. This established Peptidase M84 as a promising drug candidate for receptor mediated targeted-therapy of ovarian cancer.

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Novel indole Schiff base β-diiminato compound as an anti-cancer agent against triple-negative breast cancer: In vitro anticancer activity evaluation and in vivo acute toxicity study

Farghadani,

Lim,

Abdulla

et al. 2024

Bioorganic Chemistry

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A significantly non-toxic novel Cobalt(III) Schiff base complex induces apoptosis via G2-M cell cycle arrest in human breast cancer cell line MCF-7

Cited by 15 publications

References 51 publications

Revelation of potential bioactive water-soluble Boc-l-valine and imidazole appended metal complexes {M = Co(ii), Cu(ii) & Zn(ii)}: synthesis, characterization, ct-DNA binding, pBR322 cleavage, SOD mimetic, and cytotoxicity studies

Revelation of potential bioactive water-soluble Boc-l-valine and imidazole appended metal complexes {M = Co(ii), Cu(ii) & Zn(ii)}: synthesis, characterization, ct-DNA binding, pBR322 cleavage, SOD mimetic, and cytotoxicity studies

Metallo-protease Peptidase M84 fromBacillus altitudinisinduces ROS dependent apoptosis in ovarian cancer cells by targeting PAR-1

Novel indole Schiff base β-diiminato compound as an anti-cancer agent against triple-negative breast cancer: In vitro anticancer activity evaluation and in vivo acute toxicity study

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