A signal-on electrochemical aptasensor for rapid detection of aflatoxin B1 based on competition with complementary DNA

Wang, Chao; Li, Yapiao; Zhao, Qiang

doi:10.1016/j.bios.2019.111641

Cited by 108 publications

(47 citation statements)

References 34 publications

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“…The phosphate groups are negatively charged, which makes the aptamers negatively charged; thus, the immobilized aptamers repel the [Fe (CN) 6 ] 3–/4– anions from the electrode surface and also acts as a barrier for electron transfer [ 37 ]. After incubation of the CNT-ME/aptamer with cDNA, the redox current peak was slightly increased (I p = 10.94 mA), which may be due to the hybridization that makes the negatively charged Aptamer-MB distant from the electrode surface, hence increasing electron transfer near the electrode surface [ 38 ]. For the AgNP-ME, immobilizing the AgNPs also increased the peak current, which resulted in being higher compared to the CNT-ME, due to the higher conductivity of AgNPs compared to CNTs.…”

Section: Resultsmentioning

confidence: 99%

“…for instance, the difference in the number of aptamers immobilized on WE between one aptasensor and another may decrease the sensitivity. However, by subtracting the current generated from the blank sample, in this way, only the current generated because of the analyte will be measured, no matter the amount of the aptamers immobilized on the WE [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Hence, we increase the reproducibility of the aptasensors.…”

Section: Resultsmentioning

confidence: 99%

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Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

et al. 2020

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Furaneol is a widely used flavoring agent, which can be naturally found in different products, such as strawberries or thermally processed foods. This is why it is extremely important to detect furaneol in the food industry using ultra-sensitive, stable, and selective sensors. In this context, electrochemical biosensors are particularly attractive as they provide a cheap and reliable alternative measurement device. Carbon nanotubes (CNTs) and silver nanoparticles (AgNPs) have been extensively investigated as suitable materials to effectively increase the sensitivity of the biosensors. However, a comparison of the performance of biosensors employing CNTs and AgNPs is still missing. Herein, the effect of CNTs and AgNPs on the biosensor performance has been thoughtfully analyzed. Therefore, disposable flexible and screen printed electrochemical aptasensor modified with CNTs (CNT-ME), or AgNPs (AgNP-ME) have been developed. Under optimized conditions, CNT-MEs showed better performance compared to AgNP-ME, yielding a linear range of detection over a dynamic concentration range of 1 fM–35 μM and 2 pM–200 nM, respectively, as well as high selectivity towards furaneol. Finally, our aptasensor was tested in a real sample (strawberry) and validated with high-performance liquid chromatography (HPLC), showing that it could find an application in the food industry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

et al. 2020

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“…It was also shown that minor current change was observed in the presence of interfering mycotoxins. The proposed aptasensor was successfully used for the analysis of beer and white grape wine samples spiked with aflatoxin B [157]. The other example of aptasensor for aflatoxin B1 detection was elaborated by Nodoushan et al In their approach, a screen-printed carbon electrode was modified with GO and gold nanowires, which was followed by the incubation with thiol-modified single-stranded DNA that hybridized with aflatoxin B1 specific aptamer strand.…”

Section: Aflatoxin Bmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

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This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

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“…Another electrochemical aptasensor achieving rapid detection of aflatoxin B1 (AFB1) was designed and developed by Zhao [66]. A short anti-AFB1 aptamer having a methylene blue (MB) as redox tag was immobilized on the surface of a gold electrode.…”

Section: Toxinsmentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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Safety and quality are key issues for the food industry. Consequently, there is a growing demand to preserve the food chain and products against substances toxic, harmful to human health such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long time of analyses, expensive and complex procedures, and they require skilled personnel. Therefore, the development of performant electrochemical biosensors can significantly support the screening of food chain and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in the food screening and the challenges to address.

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A signal-on electrochemical aptasensor for rapid detection of aflatoxin B1 based on competition with complementary DNA

Cited by 108 publications

References 34 publications

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

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