A Short Evaluation of a New Haematological Analyser: The Cobas Argos 5 Diff

Bas, B. M.; Catsberg, M. J.; Kamp, Stefan

doi:10.1515/cclm.1993.31.9.603

Cited by 3 publications

(8 citation statements)

References 8 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data confirm the observations of prior studies evaluating both analysers in comparison with the microscopy method (2,5,8,9,11,13,14). No significant differences were observed between the mean values, although the slopes of the correlations of the automated methods vs. the microscopy method were slightly different from the line of identity.…”

Section: Discussionsupporting

confidence: 91%

“…Our observations agree with prior studies evaluating the Coulter STKS system and the COBAS Argos analyser (2,5,8,9,11,13,14,16). For monocytes, the slopes and intercepts of the regression lines between the automated methods and the microscopical differentiation differed from the line of identity, whereas better correlations were found in the comparison of the two analysers.…”

Section: Discussionsupporting

confidence: 91%

“…Within the last years several systems have been developed which allow a fast automated blood analysis including the differentiation of leukocyte subsets for clinical purposes by means of different techniques (2,(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Within the last years several systems have been developed which allow a fast automated blood analysis including the differentiation of leukocyte subsets for clinical purposes by means of different techniques (2,(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have proved the reliability of these analysers in comparison to the microscopy method and different automated techniques (2,4,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Leukocytes are counted and differentiated when passing through a special triple transducer flow cell.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The White Blood Cell Differential: Three Methods Compared

Siekmeier¹,

Bierlich²,

Jaross³

2001

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

The analysis of the automated blood cell count is an essential tool in haematological diagnostics. However, in the case of the white blood cell differential the microscopy method, although tedious, often serves as reference. We evaluated the ABX Pentra 120 Retic haematology analyser in comparison to the Coulter STKS haematology system and the microscopy method with respect to accuracy, precision and reliability. We compared 308 samples (239 samples from adults and 69 from children) including patients with oncological diseases. The comparison of the white blood cell differential revealed strong correlations between the results obtained with the ABX Pentra 120 Retic and the microscopy method, the Coulter STKS and the microscopy method and both automated methods (values of paediatric samples in parentheses; neutrophils: rs> or = 0.933 (rs > or = 0.951), lymphocytes: rs > or = 0.907 (rs > or = 0.945), monocytes: rs > or = 0.584 (rs > or = 0.459) and eosinophils: rs > or = 0.963 (rs > or = 0.966)). The analytical performance of automatic analysers for the detection of the morphological "left shift" was determined for all samples in comparison to the microscopical white blood cell differential. The sensitivity, specificity and efficiency depended strongly on the chosen threshold levels and were different for both analysers. The sensitivity for flagging a left shift increased with an increasing proportion of neutrophil bands, metamyelocytes, myelocytes and promyelocytes. Our study suggests that the ABX Pentra 120 Retic haematology analyser, as well as the Coulter STKS haematology system are useful tools for routine analysis in haematology.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The White Blood Cell Differential: Three Methods Compared

Siekmeier¹,

Bierlich²,

Jaross³

2001

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Höhere Variationskoeffizienten treten wiederum bei der Leukozytenzählung im leukopenischen Bereich (VK = 3,37%) und bei derThrombozytenzählung (VK zwischen 2,72% und 13,30%) auf. (9). Eine etwas größere Abweichung zwischen dem Argos-und dem HI-System hinsichtlich der MCV-Werte kann hier nicht abschließend beurteilt werden.…”

Section: Präzision Von Tag Zu Tagunclassified

Multicenter-Evaluierung des Cobas Argos-Hämatologie-Analyzers

Sachse¹,

Baudach²,

D³

et al. 1994

LaboratoriumsMedizin

View full text Add to dashboard Cite

Zusammenfassung:In einer Multicenter-Evaluierung in drei Laboratorien wurden die Meßgrößen Hämoglobin, Hämatokrit, Erythrozyten, Leukozyten, Thrombozyten und die berechneten Indices des Hämatologie-Analyzers Argos 5 Di ff im Vergleich zu den Ergebnissen am Analyzer H1 der Firma Bayer Diagnostic undCoulterSTKS untersucht. Die Zeitdifferenz zwischen den Messungen in Berlin und Hannover betrug 8 bis W Stunden. Der Probentransport erfolgte mit dem IC-Kurierdienst. Die Daten zur Qualitätssicherung, Linearität, Verschleppung, Probenalterung und zur Vergleichsmessung normaler und pathologisch veränderter Proben werden dargestellt und statistisch nach einem parameterfreien Verfahren ausgewertet. Die Ergebnisse der beiden untersuchten Argos Systeme zeigen im Vergleich zu den mit unterschiedlicher Meßtechnik arbeitenden Analysatoren H1und STKS, die bereits seit längerer Zeit eingeführt sind, gute Übereinstimmungen. Die Argos Analysatoren mit der menugesteuerten Software und einer automatisierten Probenzufuhr erwiesen sich als zuverlässig und betriebssicher. Die Betriebskosten liegen mit 0,28 DM/kleines Blutbild in einer marktgerechten Größenordnung. Schlüsselwörter: Kleines Blutbild -Hämatologie-Analyzer-Evaluierung -Cobas Argos Summary: A multicenter eväluation was conducted in three laboratories. The parameters hemoglobin, hematocrit, erythrocyte, leukocyte and thrombocyte count and the calculated erythrocyte indices ofthe blood cell counter Argos 5 Diffwere examined in comparison _.. with the results ofthe Bayer Diagnostic H1 and ofthe Coulter STKS blood cell counter. The time difference between the measurements in Berlin and Hannover was 8-hours. Sample transport was done by railroad express Service. The data concerning quality control, linearity, carry-over, samplestability and parallel measurementof normal and pathological specimens are presented. For statistical eväluation a nonparametric procedure was used. The results obtained with the two Argos Systems examined corresponded well with the outcome of the analyzers H1 and STKS using different techniques ofmeasurement, which were introduced some years ago. The Argos analy-'zers with their menu-operated Software and automatic sampling worked reliable and without major technical difficulties. The operating costs are about 0,28 DM/blood count, which is in accordance with the Standards of the market.

show abstract

Peripheral Blood Monocyte Counting: Towards a New Reference Method

Hübl

Andert²,

Erath³

et al. 1995

CCLM

View full text Add to dashboard Cite

Flow cytometric enumeration of monocytes stained with fluorescence-labelled monoclonal antibodies has been proposed as a possible reference method for monocyte counting. We compared precision and accuracy of monocyte counting of the Coulter STKS, the Cobas Argos 5 Diff, the 800-cell manual differential, and the Coulter Epics Profile II flow cytometer using double-staining with fluorescence-labelled monoclonal antibodies (CD45-F1TC and CD14-PE).Precision: STKS, Argos and Profile II achieved a precision analogous to a 3423-, 1298-, and 11089-cell differential, respectively, confirming the superiority of automated methods. Accuracy (136 normal and abnormal samples): Correlation of automated methods with the manual differential was good (STKS: r = 0.934, Argos 5 Diff: r = 0.808, Profile : r = 0.924; Spearman's rank correlation coefficient). The mean relative STKS monocyte result was 0.52 ± 1.63% (mean i SD) higher than the manual differential, whereas the Argos 5 Diff results were 1.22 ± 2.51% lower (p < 0.001). Profile II results showed a small bias against the manual differential (-0.18 ± 1.44%, p < 0.05).Analysing 135 healthy adult subjects on the Profile II, males were found to have a higher mean monocyte count (relative count: 6.95 ± 1.43% vs. 5.86 ± 0.98%; absolute count: 0.48 ± 0.15 X 10 9 /1 vs. 0.39 ± 0.11 X 10 9 /1, p < 0.001) and a higher and wider normal range than females (relative count: 4.97 to 9.78% vs. 4.26 to 7.81%, absolute count: 0.30 to 0.84 X 10 9 /1 vs. 0.25 to 0.65 X 10 9 /1).Flow cytometry based on fluorescence-labelled monoclonal antibodies for monocyte enumeration seems an efficient tool to evaluate the monocyte counting performance of haematology analysers and an ideal successor to the manual differential as reference method for monocyte counting. IntroductionEvaluations of the differential leukocyte count of haem-cannot be the main reason for this, as the less frequent atology analysers have often yielded satisfactory results eosinophils usually showed good results (1-8, 11). The for neutrophils, lymphocytes, and eosinophils, whereas morphological variety of monocytes definitely poses the performance of monocyte counting has been disap-problems for automated differentiating techniques, anpointing (1^8), even when studying only normal sam-other serious problem being lack of an appropriate referples (9, 10). The correlation with the reference method ence method. The value of the manual 400-cell difwas frequently poor and both accuracy and precision ferential, which is still used as reference in monocyte worse than for other leukocyte classes. Although mono-counting (12), is diminished by subjectivity of the examcytes represent a relatively small leukocyte class, this iner (13) and a low precision for smaller cell populations Eur J Clin Chem Clin Biochem 1995; 33 (No 11) Brought to you by | University of Arizona Authenticated Download Date | 7/20/15 8:35 PM

show abstract

A Short Evaluation of a New Haematological Analyser: The Cobas Argos 5 Diff

Cited by 3 publications

References 8 publications

The White Blood Cell Differential: Three Methods Compared

The White Blood Cell Differential: Three Methods Compared

Multicenter-Evaluierung des Cobas Argos-Hämatologie-Analyzers

Peripheral Blood Monocyte Counting: Towards a New Reference Method

Contact Info

Product

Resources

About