A shear-thinning hydrogel that extends in vivo bioactivity of FGF2

Ding, Xiaochu; Gao, Jin; Wang, Zhouguang; Awada, Hassan; Wang, Yadong

doi:10.1016/j.biomaterials.2016.09.026

Cited by 42 publications

(34 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is typically attributed to a non-specific inflammatory response to the implanted material. 37 Interestingly, throughout the in vivo study, less macrophages are detected around the PGS-TA implant compared to the PGS control group, but it is not statistically different (Fig. 10G).…”

Section: Resultsmentioning

confidence: 85%

“…These secreted peptides typically recruit more immune cells to the implantation site. 37, 38 In this case, the recruited cells accumulate on the implant edge, then migrate into and proliferate inside the implants and digest the implants. The recruited cells secreted certain amounts of collagen locally that formed a network structure in the implantation sites during the implant degradation, leading to mild fibrosis.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Tyramine functionalization of poly(glycerol sebacate) increases the elasticity of the polymer

Ding

Gao

et al. 2017

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

Poly(glycerol sebacate) (PGS) is an elastomer used widely in tissue engineering studies due to good biocompatibility. Hereby we report a tyramine functionalized PGS called PGS-TA. Tyramine adds a stronger physical bonding capability to PGS-TA. Tensile tests showed that the softness and toughness of the material were similar to PGS. However, PGS-TA demonstrated 16-folds increase of elastic deformations compared to PGS processed under identical conditions. The in vitro studies demonstrated that the viability, and metabolic activity of baboon smooth muscle cells were the same as those on tissue culture polystyrene. Porous subcutaneous implants of PGS-TA substantially degraded in vivo over two weeks, showing good biodegradability and biocompatibility. We expect PGS-TA to be useful for applications in tissues and organs that are subjected to large reversible mechanical deformations.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Tyramine functionalization of poly(glycerol sebacate) increases the elasticity of the polymer

Ding

Gao

et al. 2017

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…The nanomaterials can be either modified or unmodified organic and inorganic nanoparticles. 40–55 The physical interactions between biopolymer matrices and nanomaterials are also mainly classified into the above listed six categories of non-covalent bonds and sometimes together with strong physical absorption dominating the composite gelation process. Nanocomposite hydrogels are not included in the current work.…”

Section: Introductionmentioning

confidence: 99%

Weak bond-based injectable and stimuli responsive hydrogels for biomedical applications

Ding

Wang

2017

J. Mater. Chem. B

Self Cite

View full text Add to dashboard Cite

Here we define hydrogels crosslinked by weak bonds as physical hydrogels. They possess unique features including reversible bonding, shear thinning and stimuli-responsiveness. Unlike covalently crosslinked hydrogels, physical hydrogels do not require triggers to initiate chemical reactions for in situ gelation. The drug can be fully loaded in a pre-formed hydrogel for delivery with minimal cargo leakage during injection. These benefits make physical hydrogels useful as delivery vehicles for applications in biomedical engineering. This review focuses on recent advances of physical hydrogels crosslinked by weak bonds: hydrogen bonds, ionic interactions, host-guest chemistry, hydrophobic interactions, coordination bonds and π-π stacking interactions. Understanding the principles and the state of the art of gels with these dynamic bonds may give rise to breakthroughs in many biomedical research areas including drug delivery and tissue engineering.

show abstract

“…Our in vivo findings, suggesting a localised, prolonged angiogenic effect of VEGF when sequestered in Laponite as compared with an alginate carrier that facilitates burst release, have intriguing corollaries in drug delivery. Conventional hydrogel drug delivery strategies tend to target controlled, sustained release from the biomaterial over time as necessary strategy for recruiting local endogenous cell populations [27].…”

Section: Discussionmentioning

confidence: 99%

“…Clays are highly sorptive for biomolecules, a property which has been exploited in their use over many years as orally-taken gastrointestinal protectors [20], skin exfoliation agents [21,22] and as blood clotting agents [23]. More recently we [24,25] and others [26,27] Like many clays, Laponite has a long history of use in the food and cosmetic industries, suggesting that it is non-toxic and well tolerated, even at high doses. Aqueous dispersions of Laponite-mineral nanoplatelets (25 nm in diameter and ~1 nm in thickness) display selfassembly properties due to their charge anisotropy and form thixotropic (shear-thinning), clear, colloidal gels.…”

Section: Introductionmentioning

confidence: 99%

Injectable nanoclay gels for angiogenesis

Page

Clarkin

Mani

et al. 2019

Preprint

View full text Add to dashboard Cite

The retention and sustained activity of therapeutic proteins at delivery sites are goals of regenerative medicine. Vascular endothelial growth factor (VEGF) has significant potential in promoting the growth and regeneration of blood vessels but is intrinsically labile. This is exacerbated by the inflammatory microenvironments at sites requiring regeneration. For VEGF to be efficacious it may require a carrier that stabilises it, protects it from degradation and retains it at a site of interest. In this study we tested the hypothesis that injectable nanoclay gels composed of Laponite XLG can stabilise VEGF and retain it in active form for therapeutic delivery. To achieve this, VEGF was incorporated in Laponite gels and its activity tested at a range of concentrations using in vivo cell culture tubulogenesis assays and in vivo angiogenesis assays. We found that VEGF-Laponite gels enhanced tubulogenesis in a dose-dependent manner in vivo. When administered subcutaneously in vivo Laponite was retained at an injection site for up to a period of three weeks and promoted a 4-fold increase in blood vessel formation compared with alginate or vehicle controls as confirmed by CD31 staining. Notably, in contrast to alginate, Laponite gels did not release VEGF, indicating a strong interaction between the growth factor and the nanoclay, and suggesting that Laponite enhancement of VEGF efficacy is due to its retention at an implantation site over a prolonged period. Our approach provides a robust method for delivery of bioactive recombinant VEGF without the necessity for complex hydrogel or protein engineering.

show abstract

A shear-thinning hydrogel that extends in vivo bioactivity of FGF2

Cited by 42 publications

References 32 publications

Tyramine functionalization of poly(glycerol sebacate) increases the elasticity of the polymer

Tyramine functionalization of poly(glycerol sebacate) increases the elasticity of the polymer

Weak bond-based injectable and stimuli responsive hydrogels for biomedical applications

Injectable nanoclay gels for angiogenesis

Contact Info

Product

Resources

About