A severe case of contagious ecthyma in Tswana goats

Baipoledi, E.K.; Nyange, J.; Hyera, J.M.K.

doi:10.4102/jsava.v73i2.564

Cited by 7 publications

(7 citation statements)

References 6 publications

(7 reference statements)

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“…Clinical signs in the goats observed in this case study are similar to previous case reports of orf (Scagliarini A et al, 2012, Baipoledi et al 2002. Presence of lesions in the epidermis of the muzzle and lips without visible lesions on other body parts on this case study imply its low severity whereas higher severity the lesion extends to molar area of the maxilla or on the rumen mucosa as well as other parts of the body (Bouznach, A., 2013) PCR results and Clinical signs observed differentiate this case from other related diseases like blue tongue, ulcerative dermatosis and benign tumors Baipoledi et al 2002.…”

Section: Discussionsupporting

confidence: 89%

“…In young, stressed, or immunosuppressed sheep and goat, formation of severe bloody lesions can be fatal (Nitsche et al, 2005). The diagnosis is generally done on the basis of clinical signs, which are typical, but can be confused with symptoms of other vesicular diseases of sheep and goats -including foot and mouth disease (Watson 2004), bluetongue (Baipoledi, Nyange & Hyera 2002), peste des petits ruminants (Rossiter & Taylor 1994) and sheep and goat pox (Kitching 2004;Zheng et al, 2007). Other diseases that resemble ORFV include papillomatosis (Smith et al, 2002) (Lederman et al 2007;Steinhart 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic Analysis of ORF Virus from Goats in Tanzania: Short Communication

Mwanandota¹,

Macharia²,

Mpelumbe-Ngeleja³

et al. 2016

ujar

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ORF virus is a double-stranded DNA virus belonging to the genus Parapoxvirus (PPV) in the family poxviridae. ORF virus is the causative agent of contagious pustular dermatitis which is a zoonotic and neglected disease of humans and small ruminants. It causes severe exanthematous dermatitis that afflicts domestic and small wild ruminants. Cases of ORFV infections in goats in Tanzania have been reported for many years. The basis of reporting ORFV cases were mainly on clinical signs with no confirmatory tests (Ministry of Livestock Development reports-Tanzania). In this study, a case of proliferative dermatitis in goats was confirmed using PCR. A physical examination of the animal was carried out and tissue scrapings were collected for molecular analysis in the laboratory. The presence of ORFV in tissue scrapings from the lips was tested by GIF/IL-2gene polymerase chain reaction (PCR), DNA sequencing and phylogenetic analysis of the GIF/IL-2 gene. The results of this study indicated that the disease was caused by infection with a virus which is closely and genetically related to several ORF virus found in the data base. This is the first report to provide phylogenetic information about the ORF virus in Tanzania, which will be useful in prospective public health studies.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of ORF Virus from Goats in Tanzania: Short Communication

Mwanandota¹,

Macharia²,

Mpelumbe-Ngeleja³

et al. 2016

ujar

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show abstract

“…In Botswana, poxvirus diseases in ruminants have received little attention, with no further characterization methods performed. The first publication on poxvirus diseases in Botswana was on ORFV in goats [ 13 ], without molecular characterization of the virus. Lumpy skin disease was first observed in 1943 following an outbreak in Ngamiland in northern Botswana.…”

Section: Introductionmentioning

confidence: 99%

First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana

Modise

Settypalli

Kgotlele

et al. 2021

Virol J

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Background Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens. Methods A high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes. Results The HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR multiple sequence alignments showed that the LSDV sequences of Botswana were similar to common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel and cattle PCPV isolates. The Botswana ORFV sequence isolated from goat differed from the ORFV sequence isolated from sheep. Conclusions This study is the first report on the genetic characterization of poxvirus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose poxvirus diseases of ruminants.

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“…In Botswana, pox virus diseases in ruminants seem to be receiving little or no attention. The rst publication on pox virus diseases in Botswana was on orf virus in goats (9), without molecularly characterizing the virus. Lumpy skin disease was rst observed in 1943 following an outbreak in Ngamiland in northern Botswana.…”

Section: Introductionmentioning

confidence: 99%

First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

Modise

Settypalli²,

Kgotlele

et al. 2021

Preprint

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BackgroundPoxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former presenting also zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, and there was no prior attempt of using molecular tools to diagnose the diseases and characterize the pathogens.MethodsA high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in samples from four cattle (from Mahalapye, Kasane, and Molepolole), one sheep (from Jwaneng), and one goat (from Kasane). Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.ResultsThe HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR, and the inspection of the multiple sequence alignments showed that the LSDV sequences of Botswana were more like those of common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel isolates and cattle isolates of the PCPV group. The Botswana ORFV from goat differed from the ORFV collected in sheep.ConclusionsThis study is the first report on the genetic characterization of pox virus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose pox virus diseases of ruminants.

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A severe case of contagious ecthyma in Tswana goats

Cited by 7 publications

References 6 publications

Phylogenetic Analysis of ORF Virus from Goats in Tanzania: Short Communication

Phylogenetic Analysis of ORF Virus from Goats in Tanzania: Short Communication

First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana

First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

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