A Seroprevalence Study of Brucellosis in Goats at Kafrelsheikh Governorate, Egypt

Abstract

A random survey to study the seroprevalence of caprine brucellosis has been carried out during January to May 2011 in Matbool village of Kafrelsheikh Governorate, Egypt. A total number 276 sexually mature goats blood sera were collected from randomly sporadic 12 flocks of accessible unvaccinated goats to be analyzed. Rose Bengal Plate Test (RBPT) and indirect ELISA (I-ELISA) were used to screen all serum samples. On herd level, out of 12 flocks examined, 8 (66.66%) and 9 (75.0%) flocks were seropositive by RBPT and I-ELISA respectively. On animal level, overall seroprevalence of brucellosis among examined goats were 6.16% (n=17), and 7.97% (n=22) by RBPT and I-ELISA respectively. A non significant differences were found between the examined goats in relation to sex (p=0.8). As out of examined 112 male goats sera, 5.36% (n=6) and 7.14% (n=8) by RBPT and I-ELISA respectively, while for 164 female goats sera, 6.7% (n=11), and 8.54% (n=14) by RBPT and I-ELISA respectively. These results indicate high specificity of I-ELISA for brucella diagnosis in comparison to RBT.