A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging

Analyzing the Effect of Substituents on the Photophysical Properties of Carbazole-Based Two-Photon Fluorescent Probes for Hypochlorite in Mitochondria

“…The structures and names of the investigated molecules in this work are presented in Figure . A series of carbazole-based derivatives can be obtained by attaching different substituents at the R1 and R2 positions on the carbazole core. ,, The two-photon fluorescent properties of both probes HCM and HCH including reaction products (HCM–CNO and HCH–CNO) were experimentally measured . Both of them possess large two-photon absorption cross sections (δ TPA ); nevertheless, the properties of HCH–CNO are better.…”

“…In previous research, Wu et al experimentally reported that the length of the terminal alkyl chain has a certain degree of influence on molecular optical performances. 24 Cho and his coworkers have researched the effect of chain length for membrane probes. 25 Nevertheless, the impact of the alkyl chain length on the two-photon nature and the fluorescent efficiency of the probe has not been thoroughly studied.…”

“…It is very likely to bring a new perspective for the design of excellent two-photon fluorescent probes by utilizing alkyl modification. In previous research, Wu et al experimentally reported that the length of the terminal alkyl chain has a certain degree of influence on molecular optical performances . Cho and his co-workers have researched the effect of chain length for membrane probes .…”

A Single Fluorescent pH Probe for Simultaneous Two-Color Visualization of Nuclei and Mitochondria and Monitoring Cell Apoptosis

“…Obviously, such SF-probes should meet three criteria: (1) suitable binding affinity to DNA, (2) a positively charged π-conjugated structure to target the mitochondria, and (3) a pH-responsive site. Hydroxylated hemicyanines are an important family of pH-responsive probes, and these positively charged probes can selectively stain the mitochondria by electrostatic interactions. − On the other hand, probes with benzothiazolium salt, such as cyanine dyes and thiazole orange, could intercalate to DNA to show a high binding affinity to DNA in the nuclei. , Thus, we anticipated that the combination of hydroxylated hemicyanine and benzothiazolium salt could render a SF-probe with a dual targeting ability in nuclei and mitochondria, as well as retain their pH-responsive property to achieve two different emission colors.…”

“…35−37 On the other hand, probes with benzothiazolium salt, such as cyanine dyes and thiazole orange, could intercalate to DNA to show a high binding affinity to DNA in the nuclei. 38,39 Thus, we anticipated that the combination of hydroxylated hemicyanine and benzothiazolium salt could render a SF-probe with a dual targeting ability in nuclei and mitochondria, as well as retain their pH-responsive property to achieve two different emission colors.…”