A serially transplantable human giant cell glioblastoma that maintains a near-haploid stem line

Bigner, Sandra H.; Mark, Joachim; Schold, S. Clifford; Eng, Lawrence F.; Bigner, Darell D.

doi:10.1016/0165-4608(85)90064-0

Cited by 27 publications

(10 citation statements)

References 17 publications

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“…In accordance with BONNIN and RUBIN-STEIN [3] and KIMURA et al [19], GFAP proved to be superior to S100P. DuFvY et al [5] and BIGNER et al [2] pointed out, that monster cells contained only moderate amounts of GFAP. This is confirmed by our findings.…”

Section: Discussionsupporting

confidence: 69%

Contribution to the problem of giant cell astrocytomas

Müller

Slowik²,

Firsching

et al. 1987

Neurosurg. Rev.

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Histological, immunocytochemical, and biological features of 38 giant cell gliomas were investigated. The invasion of these tumors and its giant cells by histiocytes, lymphocytes, plasma cells, and especially by eosinophilic granulocytes is viewed as an immune response, which may explain a favorable clinical course. Fifty-three percent of the patients were younger than 45 years at the time of surgery. The average postoperative survival of 27.4 months was clearly longer than in glioblastoma. These biological features suggest a differentiation from glioblastoma multiforme. The classification of this entity as "monstrocellular astrocytoma" is proposed.

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Section: Discussionsupporting

confidence: 69%

Contribution to the problem of giant cell astrocytomas

Müller

Slowik²,

Firsching

et al. 1987

Neurosurg. Rev.

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“…Furthermore, a small but constant pool of multinucleated giant cells continues to synthesize DNA in vitro. This is in agreement with the maintenance of such giant cells in in vivo implants in nude mice (Bigner et al, 1985). That such cells are viable is further supported by the observation that their presence in cultured human glioma cell lines can be correlated with the tumourigenicity of these lines in athymic nude mice .…”

Section: Discussionsupporting

confidence: 82%

“…The tumour is composed of two cell populations and is unique in that its cells possess a near-haploid stem line. Although attempts to carry it in monolayer culture have been unsuccessful in the past (Bigner et al, 1985), we were able to maintain it in matrix cultures for up to 65 days. The matrix system constitutes a relevant in vitro model for the study of human and experimental tumours because it reproduces the three-dimensional pattern of histological organization seen in vivo Sipe, Herman & Rubinstein, 1973;Ibayashi et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Bigner and his group established a serially transplantable tumour which was derived from a human giant cell glioblastoma, maintained subcutaneously in nude mice and designated D-212 MG. It continued to express G F A protein in in uiuo transplants and demonstrated a near-haploid karyotype (Bigner et al, 1985). To our knowledge, the tumour has hitherto been refractory to in uitro growth or maintenance, and its cell kinetics have not been previously studied.…”

Section: Introductionmentioning

confidence: 94%

“…Human giant cell glioblastoma cells (designated D-212 MG) were obtained from two of us (DDB and HSF). This tumour was derived from a human giant cell glioblastoma and was established as a serially transplantable subcutaneous tumour in athymic nude mice (Bigner et al, 1985). The transplantation technique into nude mice has previously been described (Bigner et al, 1985;Jones et al, 1981).…”

Section: Details Of the Matrix Culture Techniquementioning

confidence: 99%

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Kinetics and glial fibrillary acidic (GFA) protein production in a transplantable human giant cell glioblastoma (D‐212 MG) of near haploid karyotype maintained in an organ culture system. An immunohistochemistry study

Ibayashi

Herman

Boyd

et al. 1990

Neuropathology Appl Neurobio

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A transplantable subcutaneous tumour (designated D-212 MG), sequentially passaged in athymic nude mice and originally derived from a human giant cell glioblastoma, was maintained in an organ culture (matrix) system and studied immunohistochemically after in vitro pulse-labelling with bromodeoxyuridine (BrdU) and for the presence of glial fibrillary acidic (GFA) protein, after 1, 2 and 3 weeks in culture. The histological characteristics of the tumour, showing two cell populations of giant multinucleated cells and small cells, were preserved in the explants. An increased percentage of multinucleated giant cells was found after 3 weeks in vitro. A small but constant fraction (4-6%) of these cells continued to synthesize DNA. The labelling index of the small cells was somewhat higher, but decreased slightly although significantly over the 3-week period in vitro (from approximately 10.5 to 8%). The percentage of small cells that were positive for GFA protein was in the region of 75% and that of the giant multinucleated cells was in the region of 45%; it did not change significantly during the 3 weeks in vitro. The in vitro results confirm the astrocytic nature of both the small cells and the giant multinucleated cells in this tumour, the capacity of both cell populations to synthesize DNA in culture and to demonstrate invasiveness, and suggest the possibility that some of the giant multinucleated cells may have originated from the conversion of a number of small tumour cells.

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Near‐haploid karyotype in a squamous cell lung carcinoma

Drouin¹,

Viguié²,

Debesse

1993

Genes Chromosomes & Cancer

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Cytogenetic analysis of a squamous cell lung carcinoma revealed a near-haploid karyotype with 27 chromosomes, in both primary cultures and an established cell line. The only chromosomes with two homologs present were chromosomes X, 5, 7, and 22. The two X chromosomes were early and late replicating, respectively. No structural rearrangements could be detected. In vitro, the clone evolved by duplication towards hyperdiploidy with 54 chromosomes.

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A serially transplantable human giant cell glioblastoma that maintains a near-haploid stem line

Cited by 27 publications

References 17 publications

Contribution to the problem of giant cell astrocytomas

Contribution to the problem of giant cell astrocytomas

Kinetics and glial fibrillary acidic (GFA) protein production in a transplantable human giant cell glioblastoma (D‐212 MG) of near haploid karyotype maintained in an organ culture system. An immunohistochemistry study

Near‐haploid karyotype in a squamous cell lung carcinoma

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