Near‐haploid karyotype in a squamous cell lung carcinoma

Drouin, Valérie; Viguié, F; Debesse, B

doi:10.1002/gcc.2870070405

Cited by 17 publications

(6 citation statements)

References 18 publications

(18 reference statements)

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“…Interestingly, 50% of these benign teratomas changed to chorio-carcinomas, which might be a case for changes to "new" functional genes from genomic-duplications (Wolfe, 2001). Another example of in vivo haploid-associated proliferation is from leukemia and some solid tumors (Drouin et al, 1993;Mitelman, 1988). From Mitelman's Catalog (1988) some karyotype-examples for different cases showing near-haploidy are listed in Table 2.…”

Section: Discussionmentioning

confidence: 99%

Normal Human Cells Acquiring Proliferative Advantage to Hyperplasia-Like Growth-Morphology: Aberrant Progeny Cells Associated With Endopolyploid and Haploid Divisions

Walen¹

2013

CCO

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Today, cancer-patients can seek individual genomic tailored therapy, but at escalating costs. However, cancer-incidence reduction would reduce such health-cost. The essence of this report is a cell-culture experimental approach for the production of genetically unstable cells from normal cells which with accumulation of “mutations” might reveal early, neoplastic-like pathology. Summarily the experimental focus was on: 1) establishment of a simple nutritional method (amino acid deprivation) for young-cell-response with depoly-ploidization of endopolyploid cells to diploid CIN-cells, 2) a first time observation of a fast growing new, small cell-type, appearing time-correlated with haploid segregations in normal, fibroblast metaphases and 3) growth characteristics of the progeny cells in 1) and 2) which from their young-cell derivation, could be growth-extended in cultural passages. This would hypothetically model early tumorigenic progression from normal cells. These studies revealed gain of proliferative advantage (GPA), multilayered, changed patterns of growth, focal 3-D cell-heaps with cell polarity change, and star-like mitotic forms. Comparatively, these developments were typical of hyperplasias. The transiency of the originating genome reductive mechanisms may have prevented earlier detections. It is discussed that present growth-changes were responses to homozygous-caused loss of heterozygosity (LOH) of putative pro-proliferation genetics, whereas leukemic and some solid tumor cells showed hemizygous-caused LOH in near-haploid clonal proliferations. The practical outcome is an experimentally simple, nutrition-based model system for SNP-based haplo-karyotyping of the two aberrant cell-types with GPA, which is a cancer, fundamental trait. Never before have cellular mechanism been shown to initiate in normal, human cells, neoplastic-like proliferative responses.

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Section: Discussionmentioning

confidence: 99%

Normal Human Cells Acquiring Proliferative Advantage to Hyperplasia-Like Growth-Morphology: Aberrant Progeny Cells Associated With Endopolyploid and Haploid Divisions

Walen¹

2013

CCO

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“…We were surprised to notice the lack of overlap between lost chromosomal regions in CIN2/3 lesions and SCCs. A number of other studies investigating lung, colorectal, and breast cancer postulated the concept of chromosome loss and subsequent reduplication in epithelial carcinogenesis (53)(54)(55)(56). This concept implies that, although losses detected in premalignant lesions are no longer present in invasive carcinomas, tumor suppressor genes located within these previously lost regions are still silenced through other mechanisms in more advanced stages of disease, such as allelic loss and potentially epigenetic modifications, all of which may remain undetected by array CGH.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Signatures of a Subset of High-Grade Premalignant Cervical Lesions Closely Resemble Invasive Carcinomas

Wilting¹,

Steenbergen²,

Tijssen³

et al. 2009

Cancer Research

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Cervical cancer develops from precancerous high-grade cervical intraepithelial neoplasia (CIN) harboring a transforming infection with high-risk human papillomavirus, which is characterized by p16INK4a overexpression. Once such a lesion has developed, progression toward an invasive squamous cell carcinoma (SCC) may take one or more decades, underlining the heterogeneity of these lesions in terms of duration of existence and progression risk. We performed array-based comparative genomic hybridization (array CGH) on 46 p16

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“…We believe that these cases do indeed represent true examples of inflammatory LMS, since not only do they satisfy the morphologic criteria of inflammatory LMS, but also one of the cases has the characteristic near-haploid karyotype similar to that seen in previously published examples of inflammatory LMS (Table 4) [2]. A near-haploid karyotype is rarely observed in human cancers, and has been reported to occur only in a subset of acute lymphoblastic leukemias with a poor prognosis [10], some cases of chronic myeloid leukemia in blast crisis [11,12], and rare cases of endometrioid carcinoma [13], pancreatic endocrine tumor [14], papillary and follicular carcinoma of the thyroid [15], renal oncocytoma [16], sacrococcygeal teratoma [17], pulmonary squamous cell carcinoma [18], chrondrosarcoma [19], and pleomorphic/storiform malignant fibrous histiocytoma [20]. Notably, conventional LMS has not been reported to have a near-haploid karyotype and in general, has complex, hyperdiploid karyotypes [21,22].…”

Section: Discussionmentioning

confidence: 99%

So-Called “Inflammatory Leiomyosarcoma”: A Series of 3 Cases Providing Additional Insights into a Rare Entity

et al. 2005

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Inflammatory leiomyosarcoma, a rare entity first described in 1995, has been characterized by smooth muscle differentiation, a near-haploid karyotype, and a surprisingly good prognosis. The morphology is similar to that of conventional leiomyosarcoma admixed with a chronic inflammatory infiltrate. Thus far, only 15 cases have been reported in the English language literature. We report the clinical and pathological features of 3 additional cases of inflammatory leiomyosarcoma. Two women (ages 64 and 25, respectively) and 1 man (age 32) presented with a thigh, ovary, and lung mass, respectively. Inflammatory symptoms, such as anorexia, fever, night sweats, abdominal pain, and diarrhea, coincided with the thigh and ovarian primaries. Immunohistochemical studies revealed diffuse positivity for desmin and poor expression for other smooth muscle and skeletal muscle markers (muscle-specific actin [0/3], alpha-smooth muscle actin 1/3 [focal], calponin [1/3], caldesmon [0/3], and myogenin [0/3]). CD68 was diffusely positive in both the histiocytes and spindle cell component in all cases. Ultrastructural evaluation of 1 case (lung primary) lacked definitive smooth muscle differentiation. Cytogenetic analysis in 1 of 2 cases that were karyotyped, identified a near-haploid karyotype, which has been reported in other cases of inflammatory leiomyosarcoma. The other case showed 2 clonal populations of cells with interstitial deletions of the short arm of chromosome 8 and the long arm of chromosome 9, respectively. The case without cytogenetic data was intimately associated with an ovarian mature teratoma. These data also suggest that inflammatory leiomyosarcoma may lack smooth muscle differentiation, characterized by diffuse immunoreactivity for desmin but lack of immunoreactivity for alpha-smooth muscle actin, calponin, and caldesmon. In addition, 2 of the 3 cases developed distant metastases to the lungs, which suggests that these lesions may have a worse prognosis than previously believed.

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Near‐haploid karyotype in a squamous cell lung carcinoma

Cited by 17 publications

References 18 publications

Normal Human Cells Acquiring Proliferative Advantage to Hyperplasia-Like Growth-Morphology: Aberrant Progeny Cells Associated With Endopolyploid and Haploid Divisions

Normal Human Cells Acquiring Proliferative Advantage to Hyperplasia-Like Growth-Morphology: Aberrant Progeny Cells Associated With Endopolyploid and Haploid Divisions

Chromosomal Signatures of a Subset of High-Grade Premalignant Cervical Lesions Closely Resemble Invasive Carcinomas

So-Called “Inflammatory Leiomyosarcoma”: A Series of 3 Cases Providing Additional Insights into a Rare Entity

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