A Sequential Enzymatic Microreactor System for Ethanol Detection of Gasohol Mixtures

Alhadeff, Eliana M.; Salgado, Andréa Medeiros; Pereira, Nei; Valdman, Belkis

doi:10.1007/978-1-59259-991-2_31

Cited by 4 publications

(7 citation statements)

References 20 publications

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“…A significant outcome of this study was the finding that the kinetics of enzyme was the same in a solution and after immobilization on the surface of beads. More recently, Alhadeff et al [21] showed that sequential enzymatic double microreactor system with alcohol oxidase (AOD) and horseradish peroxidase (HRP) immobilized on aminopropyl glass beads could be used to quan- tifying ethanol from gasohol mixture. The sequential system was composed of two microreactors made of acrylic.…”

Section: Introductionmentioning

confidence: 99%

LTCC microreactor for urea determination in biological fluids

Malecha

Pijanowska

Gołonka

et al. 2009

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

LTCC microreactor for urea determination in biological fluids

Malecha

Pijanowska

Gołonka

et al. 2009

Sensors and Actuators B: Chemical

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“…Flow analysis techniques have also been applied in method development for ethanol analysis. Alhadeff et al reported some enzymatic methods using flow-based techniques for determination of ethanol contents in gasohol fuels [8,9] and in fermentation bioprocess [10]. This paper reports the development of a new method for quantitative analysis of ethanol in gasohol.…”

Section: Introductionmentioning

confidence: 99%

“…In principle, the method in this paper should be more robust than use of the enzymes [8][9][10], and the method has a good potential in further development for making a portable device for quality control of gasohol.…”

Section: Introductionmentioning

confidence: 99%

Quality control of gasohol using a micro-unit for membraneless gas diffusion

Muncharoen

Sitanurak²,

Tiyapongpattana³

et al. 2008

Microchim Acta

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This work describes development of a new spectrophotometric flow analysis technique suitable for monitoring of ethanol content in gasohol fuel. In this technique, the concept of membraneless gas-diffusion (MBL-GD) was applied with one-step aqueous extraction of gasohol (1:2 gasohol:water). Segments of aqueous extract and color developing reagent were allowed to flow into two separate channels in the MBL-GD device. Inside the device, ethanol vapor can diffuse across a small headspace between the two channels (donor and acceptor). Introduction of an air-segment behind the zone of acceptor reagent to stop dispersion of the colored zone has greatly improved the rapidity of analysis using this MBL-GD technique. Two methods were developed for quality control of gasohol by measuring ethanol content. Method I is suitable for direct calibration of E5 and E10. Method II is recommended for E20. These methods have high accuracy with good precision (% RSD: 1 to 4.9, n=45) and have a sample throughput of 26 samples h -1 . E10 samples were compared with analysis using a standard GC method.

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“…Methods for monitoring the percentage of ethanol in the mixtures (product quality control) or in case of spilling of gasohol blends need to be develop. Many efforts for different potentiometric, amperometric, and spectrophotometric biosensors have been developed for ethanol analyses, with microorganisms like Gluconobacter oxydans, Saccharomyces ellipsoideus, or enzymes as alcohol dehydrogenase or alcohol oxidase were just reported in the literature [17]. In Brazil, there is a rigorous program for a strict control of the physicochemical characteristics of the gasohol blend and hydrated fuel alcohol to prevent adulteration or environmental contamination.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, the 4.0 industry claims for more robust and sensitive instruments for long-distance transmission and data transfer systems to an analytical central station in monitoring and process control program. Only a few biosensors are commercially available at present for analysis control and the integration of nanomaterials composites within these enzymatic biosensors brings new strategies for enhancing their analytical performances [16,17]. The high ethanol solubility turns the assessment and analytical methods limited.…”

Section: Introductionmentioning

confidence: 99%

Development of an Ethanol Biosensor Based on Silver Nanoparticles/Polyaniline/Graphite/Epoxy Composite for Friendly Analytical Application

Alhadeff¹,

Bosco²,

Pastusiak³

et al. 2019

Biosensors for Environmental Monitoring

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Electrochemical biosensors for measuring ethanol were developed in this study. Silver nanoparticles were incorporated to increase sensibility. Firstly, the transducer element was prepared with silver nanoparticles/polyaniline/graphite/epoxy composite (AgNPs/PANI/GEC) where the enzymes alcohol oxidase (AOD) and horseradish peroxidase (HRP) were immobilized by adsorption. The composition of the immobilized solution was indicated by an experimental design (85% of the bi-enzymatic solution, 10% of albumin and 5% of glutaraldehyde). The immobilization method adopted in this study showed to be highly reproducible. The values of variance and standard deviation were low (0.003 and 0.053, respectively-means of three electrodes). The linear range was 0-30 g/L (R 2 = 0.983) and the sensitivity 0.004 mA L/g. A second biosensor was made with a transducer prepared with AgNPs/PANI/GEC. A chitosan film was applied over the adsorbed enzymatic solution to avoid desorption of the immobilized enzymes. The AgNPs/PANI/GEC electrodes were characterized using cycle voltammetry and the composite surface by scanning electron microscopy. The calibration for ethanol samples with this second composite fitted in a range of 0.0-0.35 g/L (R 2 = 0.984). Square rate voltammetry was the electrochemical analytical method used to obtain the amperometric calibration curves.

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A Sequential Enzymatic Microreactor System for Ethanol Detection of Gasohol Mixtures

Cited by 4 publications

References 20 publications

LTCC microreactor for urea determination in biological fluids

LTCC microreactor for urea determination in biological fluids

Quality control of gasohol using a micro-unit for membraneless gas diffusion

Development of an Ethanol Biosensor Based on Silver Nanoparticles/Polyaniline/Graphite/Epoxy Composite for Friendly Analytical Application

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