A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations

Abstract

A new, simple and sensitive spectrophotometric method for the determination of some sulfonamide drugs has been developed. The method is based on the diazotization of sulfacetamide, sulfadiazine, sulfaguanidine, sulfamerazine, sulfamethazine, sulfamethoxazole, and their coupling with 8-hydroxyquinoline in alkaline media to yield red coloured products with absorption maxima at 500 nm. Beer's law is obeyed from 0.1-7.0 microg mL-1. The limits of quantification and limits of detection were 0.11-0.18 and 0.03-0.05 microg mL-1, respectively. Intraday precision (RSD 0.1-0.5%) and accuracy (recovery 97.3--100.8%) of the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the assay of sulpha drug in pharmaceutical formulations.

Synthesis, spectral characterization, and antimicrobial studies of metal complexes of the Schiff base derived from [4-amino-N-guanylbenzene sulfonamide] and salicylaldehyde

“…Schiff-base metal complexes involving sulfaguanidine and salicylaldehyde are expected to possess enhanced biological activities [1][2][3][4][5][6][7][8][9][10][11]. In this connection, sulfaguanidine condensed with salicylaldehyde offers an extension to the study since perturbation in the ligand backbone may affect the stereochemistry of the complexes.…”

“…Apart from being innocuous to human beings, they are efficient against pathogenic germs. Several spectrophotometric methods for determining sulfonamides in pharmaceutical preparation exist [6][7][8]. Their Schiff bases and complexes were investigated for physiologically relevant carbonic anhydrase [9].…”

Eriochrome Black T – a New Analytical Reagent for Spectrophotometric Determination of Sulphanilamides

“…They are based on the interaction of both SA [1][2][3][4][5] and SA diazonium salts [6][7][8][9][10][11][12][13] with organic reagents leading to formation of the colored analytical forms. Even though dyes are common analytical reagents in pharmaceutical analysis, they are rarely used for SA determination.…”

Development and validation of the simple and sensitive spectrophotometric method of sulphanilamides determination with 4-(2-thiazolylazo)-resorcinol in veterinary preparations

“…The conditions of SA diazotization described in the literature are rather contradictory (either hydrochloric acid in the concentration range from 0.1 M [46] to 5 M [47][48][49] or 10 M sulphuric acid [50][51][52] or a mixture of phosphoric and acetic acids [53] is used), so we have investigated the influence of various factors on the SA diazotization, such as the influence of the nature and concentration of acid for diazotation medium, diazotizing reagent, the temperature and the reaction time. As follows from the experimental data at Fig.…”