A sensitive RP-HPLC method for estimation of artemether from polymeric nanoparticles after pre-column acid treatment using UV-visible detector

Bhide, Atharva R; Surve, Dhanashree H.; Guha, Sonia; Jindal, Anil

doi:10.1080/10826076.2020.1777564

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Coumarin-6- or DIR-loaded surrogate nanospheres and nanorods were prepared as described above, omitting artemether. Nanospheres were characterized for particle size and polydispersity index (PDI) using a Malvern Nano ZS (Malvern Instruments Ltd., UK), % entrapment efficiency and % drug loading were determined by estimating the drug in nanorods using the high-performance liquid chromatography-based analytical method, and shape was determined by scanning electron microscopy (SEM) (FEI scanning electron microscope, Hillsboro, Washington). On the other hand, nanorods were also characterized for % drug content, major and minor Feret’s diameter, major and minor axis, and aspect ratio using ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, nanorods were found to be suitable for intravenous delivery of artemether. 22 Hence, the objective of the present study was the evaluation of pharmacokinetics, biodistribution, and antimalarial efficacy of artemether loaded in polymeric nanorods. The in vivo efficacy of artemetherloaded nanorods was studied in Plasmodium berghei infected mouse model.…”

Section: ■ Introductionmentioning

confidence: 99%

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Evaluation of Pharmacokinetics, Biodistribution, and Antimalarial Efficacy of Artemether-Loaded Polymeric Nanorods

et al. 2022

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Artemether oily injection is recommended for the treatment of severe malaria by the intramuscular route. The major limitations of the artemisinin combination therapy are erratic absorption from the injection site and high dosing frequency due to a very short elimination half-life of the drug. Advanced drug delivery systems have shown significant improvement in the current malaria therapy; the desired drug concentration within infected erythrocytes is yet the major challenge. Recently, we have reported the fabrication of artemether-loaded polymeric nanorods for intravenous malaria therapy which was found to be biocompatible with THP-1 monocytes and rat erythrocytes. The objective of the present study was the evaluation of pharmacokinetics, biodistribution, and antimalarial efficacy of artemether-loaded polymeric nanorods. Scanning electron microscopy and confocal microscopy studies revealed that both nanospheres and nanorods were adsorbed onto the surface of rat erythrocytes after an incubation of 10 min. After intravenous administration to rats, artemether nanorods showed higher plasma concentration and lower elimination rate of artemether when compared with nanospheres. The biodistribution studies showed that, at 30 min, the liver concentration of DiRloaded nanospheres was higher than that of DiR-loaded nanorods after intravenous administration to BALB/c mice. The in vitro schizont inhibition study showed that both nanorods and nanospheres exhibited concentration-dependent parasitic inhibition, wherein at lower concentrations (2 ppm), nanorods were more effective than nanospheres. However, at higher concentrations, nanospheres were found to be more effective. Nanorods showed higher chemosuppression on day 5 and day 7 than nanospheres and free artemether when studied with the Plasmodium berghei mouse model. Moreover, the survival rate of P. berghei infected mice was also found to be higher after treatment with artemether nanoformulations when compared with free artemether. In conclusion, polymeric nanorods could be a promising next-generation delivery system for the treatment of malaria.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%