A Sensitive Method for the Determination of Baclofen in Human CSF by High Performance Liquid Chromatography

Abstract

Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information:Un iversiti Paul Sa batier 31400 Toulouse Cedex, France 2Lab orato ire de Neuro ech irurg-ee et Neuro b io logie A ppliq uie ABSTRACT Baclofen (4-aminc+3pchlorophenylbutyric acid) is used clinically for the treatment of multiple sclerosis and other spastic conditions. The intrathecal route Df abinistration is now prefered to the o r a l route. To optimize efficacy, the levels of the drug in CSF need to be monitored after intrathecal ahministration. In this paper, the authors describe a sensitive reverse phase high performance liquid chromatographic method for the determination of baclofen in human CSF. This =say employed cation exchange extraction, precolumn derivatization with PITC, and ultra-violet detection 1753