A sensitive LC/MS/MS assay of 25OH vitamin D3 and 25OH vitamin D2 in dried blood spots

Eyles, Darryl W.; Anderson, Charles T.; Ko, Pauline; Jones, Alun; Thomas, A; Burne, Thomas H. J.; Mortensen, Preben Bo; Nørgaard‐Pedersen, Bent; Hougaard, David M.; McGrath, John

doi:10.1016/j.cca.2009.02.005

Cited by 218 publications

(227 citation statements)

References 36 publications

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“…2). The 25OHD levels detected by LC-MS/MS correlate well with 25OHD levels obtained by radioimmunoassay (36,37); however, they are somewhat lower because LC-MS/MS can distinguish 25OHD from other vitamin D metabolites (e.g., 24,25OHD, lactone form) (97). A chemistry panel was also performed on isolated serum (IDEXX RADIL, Research Animal Diagnostic Laboratory at the University of Missouri) ( Table 1 and Table S1).…”

Section: Methodsmentioning

confidence: 76%

“…To determine if dietary manipulation of VitD3 affected vitamin D status, blood levels of 25OHD were determined in a subset of animals using a blood spot LC-tandem MS (MS/MS)-based method (36,37). 25OHD is the most common indicator of vitamin D status because it is a stable and long-lived metabolite of vitamin D. Results showed that circulating 25OHD levels were proportional to the amount of VitD3 in the diet.…”

Section: Significancementioning

confidence: 99%

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Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats

Latimer¹,

Brewer²,

Searcy³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

180

103

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Vitamin D is an important calcium-regulating hormone with diverse functions in numerous tissues, including the brain. Increasing evidence suggests that vitamin D may play a role in maintaining cognitive function and that vitamin D deficiency may accelerate agerelated cognitive decline. Using aging rodents, we attempted to model the range of human serum vitamin D levels, from deficient to sufficient, to test whether vitamin D could preserve or improve cognitive function with aging. For 5-6 mo, middle-aged F344 rats were fed diets containing low, medium (typical amount), or high (100, 1,000, or 10,000 international units/kg diet, respectively) vitamin D3, and hippocampal-dependent learning and memory were then tested in the Morris water maze. Rats on high vitamin D achieved the highest blood levels (in the sufficient range) and significantly outperformed low and medium groups on maze reversal, a particularly challenging task that detects more subtle changes in memory. In addition to calcium-related processes, hippocampal gene expression microarrays identified pathways pertaining to synaptic transmission, cell communication, and G protein function as being up-regulated with high vitamin D. Basal synaptic transmission also was enhanced, corroborating observed effects on gene expression and learning and memory. Our studies demonstrate a causal relationship between vitamin D status and cognitive function, and they suggest that vitamin D-mediated changes in hippocampal gene expression may improve the likelihood of successful brain aging.V itamin D, a secosteroid hormone known for its role in bone and calcium homeostasis, is now well recognized for its many diverse functions and actions on a variety of tissues and cell types (1, 2). Vitamin D typically refers to the precursor forms of the hormone obtained through the skin's exposure to sunlight [vitamin D3 (VitD3)] or from dietary sources (VitD3 or VitD2). A metabolite of vitamin D, 25-hydroxyvitamin D (25OHD), is a serum biomarker of vitamin D status or repletion. In recent years, there is particular concern that large segments of the population may have low levels of 25OHD, and therefore are vitamin D-deficient (3). Due to factors such as reduced intake, absorption, and decreased exposure to sunlight, aging adults (≥50 y of age) are especially susceptible (3-6). Notably, this predisposition for lower 25OHD levels in the elderly has been linked to higher risk for numerous age-related disorders, including cancer and metabolic and vascular diseases (7-10).Inadequate vitamin D status also correlates with a greater risk for cognitive decline in the elderly (4,(11)(12)(13)(14)(15), suggesting that optimal levels may promote healthy brain aging (16,17). Because the brain expresses vitamin D receptors (VDRs) and can synthesize the active form of the hormone, the possible cognitive enhancing effects of vitamin D may reflect a primary action in the brain rather than a result of secondary systemic effects (18)(19)(20)(21)(22). Indeed, we and others have shown that vitamin D, as ...

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Section: Methodsmentioning

confidence: 76%

Section: Significancementioning

confidence: 99%

Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats

Latimer¹,

Brewer²,

Searcy³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

180

103

View full text Add to dashboard Cite

show abstract

“…Total 25OHD was reported as the sum of 25-hydroxyvitamin D2 (25OHD2) and 25-hydroxyvitamin D3 (25OHD3) species measured in plasma using a modification of a method previously described (Eyles et al, 2009 and evaporated to dryness. Samples were derivatised using 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) and reconstituted in ACN:H 2 O (1:3) prior to analysis.…”

Section: Assessment Of Vitamin D Statusmentioning

confidence: 99%

Prevalence and predictors of vitamin D deficiency based on maternal mid-gestation and neonatal cord bloods: The Generation R Study

Vinkhuyzen

Eyles

Burne

et al. 2016

The Journal of Steroid Biochemistry and Molecular Biology

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of vitamin D deficiency based on maternal mid-gestation and neonatal cord bloods: the Generation R Study, Journal of Steroid Biochemistry and Molecular Biology http://dx.doi.org/10. 1016/j.jsbmb.2015.09.018 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ResultsThe prevalence of vitamin D deficiency at midgestation was 26%, while in neonates 46% were deficient. 21% of the mother-infant pairs had persistent vitamin D deficiency (i.e. deficient in maternal and cord samples) and an additional 29% were vitamin D deficient in one of the two samples only. Persistent vitamin D deficiency was strongly associated with non-European ancestry and spring birth. Conclusions

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“…Sample preparation and analysis was performed using liquid chromatography-mass spectrometry (LC-MS) according to a modified version of the analysis [15]. The modifications consisted of calibrations conducted by using commercially available calibrators and controls (Perkin Elmer, Waltham, MA, USA) and an online extraction step.…”

Section: Assessment Of Vitamin D Statusmentioning

confidence: 99%

Neonatal vitamin D status is not associated with later risk of type 1 diabetes: results from two large Danish population-based studies

et al. 2016

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Aims/hypothesis The aim of this work was to assess whether neonatal levels of 25-hydroxyvitamin D (25(OH)D) are associated with risk of developing type 1 diabetes before the age of 18 years. Methods Two large-scale studies with different designs-a case-cohort and a case-control-were conducted using Danish national register data and biobank material. Weighted Cox regression and conditional logistic regression were used to calculate HRs and ORs, respectively. The concentration of 25(OH)D was assessed from neonatal dried blood spots using highly sensitive liquid chromatography-tandem mass spectrometry. Quintiles of 25(OH)D 3 were used in the main analyses.Results The case-cohort study included 912 type 1 diabetes cases and 2866 individuals without type 1 diabetes born in Denmark between 1981 and 2002 and followed up until the end of 2012. The case-control study included 527 matched case-control pairs born between 1981 and 1999 and followed up until May 2004. Both studies found no association between 25(OH)D 3 levels and later risk of developing type 1 diabetes. The neonatal total 25(OH)D levels in the studies were low: 46% (case-cohort study) and 51% (case-control study) of individuals had 25(OH)D levels <25 nmol/l. Conclusions/interpretation Our two large-scale national studies showed that 25(OH)D 3 levels around the time of birth were not associated with later type 1 diabetes risk. Whether higher levels of 25(OH)D 3 during pregnancy, acquired by higher doses of supplementation than are recommended today in Ramune Jacobsen and Steffen U. Thorsen are joint first authors.Electronic supplementary material The online version of this article

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A sensitive LC/MS/MS assay of 25OH vitamin D3 and 25OH vitamin D2 in dried blood spots

Cited by 218 publications

References 36 publications

Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats

Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats

Prevalence and predictors of vitamin D deficiency based on maternal mid-gestation and neonatal cord bloods: The Generation R Study

Neonatal vitamin D status is not associated with later risk of type 1 diabetes: results from two large Danish population-based studies

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