A sensitive LC‐ESI‐Q‐TOF‐MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley

Tsednee, Munkhtsetseg; Mak, Yit-Wai; Chen, Yet-Ran; Yeh, Kuo‐Chen

doi:10.1111/j.1469-8137.2012.04206.x

Cited by 36 publications

(43 citation statements)

References 45 publications

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“…To confirm this, we examined NA secretion from barley (Hordeum vulgare) roots. In line with previously published reports (Howe et al, 1999;Suzuki et al, 2006;Tsednee et al, 2012), we found that NA was not detectable in barley root exudates, but was clearly detectable inside the root (Supplemental Fig. S1).…”

Section: Comparative Metabolite Profiling Of Root Exudatessupporting

confidence: 93%

“…We did not detect the Fe(III)-NA complex. This might be because it has lower stability than Fe(II)-NA in the condition (von Wiren et al, 1999;Tsednee et al, 2012).…”

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 98%

“…To investigate the function of the secreted NA, we first analyzed metal-NA complexes in A. halleri roots and root exudates. LC-ESI-Q-TOF-MS, in negative ESI mode, can identify negatively charged metal-NA complexes and their mass isotopic patterns (Tsednee et al, 2012 We calculated the theoretical mass and isotopic distributions of the complexes using online software (http:// fluorine.ch.man.ac.uk/; University of Manchester).…”

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 99%

“…3A). To confirm the metal-NA complexes, we used prepared metal-NA complex standards for analysis (Tsednee et al, 2012). The relative abundance of the Zn(II)-NA complex in A. halleri roots was 3-to 15-fold higher than that of the Fe(II)-NA complex under the treatment conditions (Fig.…”

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 99%

“…Unlike phytosiderophores, NA is not secreted from grasses (Howe et al, 1999;Tsednee et al, 2012;Supplemental Fig. S1).…”

Section: Figs S4 and S6) Because Free Znmentioning

confidence: 99%

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Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

et al. 2014

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Hyperaccumulators tolerate and accumulate extraordinarily high concentrations of heavy metals. Content of the metal chelator nicotianamine (NA) in the root of zinc hyperaccumulator Arabidopsis halleri is elevated compared with nonhyperaccumulators, a trait that is considered to be one of the markers of a hyperaccumulator. Using metabolite-profiling analysis of root secretions, we found that excess zinc treatment induced secretion of NA in A. halleri roots compared with the nonhyperaccumulator Arabidopsis thaliana. Metal speciation analysis further revealed that the secreted NA forms a stable complex with Zn(II). Supplying NA to a nonhyperaccumulator species markedly increased plant zinc tolerance by decreasing zinc uptake. Therefore, NA secretion from A. halleri roots facilitates zinc hypertolerance through forming a Zn(II)-NA complex outside the roots to achieve a coordinated zinc uptake rate into roots. Secretion of NA was also found to be responsible for the maintenance of iron homeostasis under excess zinc. Together our results reveal root-secretion mechanisms associated with hypertolerance and hyperaccumulation.

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Section: Comparative Metabolite Profiling Of Root Exudatessupporting

confidence: 93%

“…We did not detect the Fe(III)-NA complex. This might be because it has lower stability than Fe(II)-NA in the condition (von Wiren et al, 1999;Tsednee et al, 2012).…”

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 98%

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 99%

Section: Na Secreted From a Halleri Roots Forms A Stable Complex Witmentioning

confidence: 99%

“…Unlike phytosiderophores, NA is not secreted from grasses (Howe et al, 1999;Tsednee et al, 2012;Supplemental Fig. S1).…”

Section: Figs S4 and S6) Because Free Znmentioning

confidence: 99%

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Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

et al. 2014

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Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

et al. 2014

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For the first time the phytosiderophore 2'-deoxymugineic acid (DMA) could be accurately quantified by LC-MS/MS in plant and soil related samples. For this purpose a novel chromatographic method employing porous graphitic carbon as stationary phase combined with ESI-MS/MS detection in selected reaction monitoring was developed. Isotope dilution was implemented by using in-house synthesized DMA as external calibrant and ¹³C₄-labeled DMA as internal standard (concentration levels of standards 0.1-80 μM, determination coefficient of linear regression R² > 0.9995). Sample preparation involved acidification of the samples in order to obtain complete dissociation of metal-DMA complexes. Excellent matrix related LOD and LOQ depending on different experimental setups were obtained in the range of 3-34 nM and 11-113 nM, respectively. Standard addition experiments and the implementation of the internal ¹³C₄-DMA standard proved the accuracy of the quantification strategy even in complex matrices such as soil solution. The repeatability of the method, including sample preparation, expressed as short- and long term precision was below 4 and 5% RSD, respectively. Finally, application in the context of plant and soil research to samples from rhizosphere sampling via micro suction cups, from soil solutions and soil adsorption/extraction studies revealed a DMA concentration range from 0.1 to 235 μM.

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Mass spectrometry‐derived systems biology technologies delineate the system's biochemical applications of siderophores

Gao

Guan

et al. 2016

Mass Spectrometry Reviews

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Siderophores are chemically diverse secondary metabolites that primarily assist the host organisms to chelate iron. Siderophores are biosynthesized by many biological organisms, including bacteria, fungi, and plants and they are responsible for a variety of biological functions beyond capture iron. Thus, they could provide a novel understanding of host-pathogen interactions, plant physiology, disease pathogenesis, and drug development. However, knowledge gaps in analytical technologies, chemistry, and biology have severely impeded the applications of siderophores, and a new strategy is urgently needed to bridge these gaps. Mass spectrometry (MS) and associated technologies render unparalleled advantages in this niche in terms of high throughput, resolution, and sensitivity. Herein, this critical review briefly summarizes progress in the study of siderophores and specifically identifies MS-based novel strategies that attempt to mimic the complexity of siderophore systems in order to increase the applicability of these compounds in the scientific community. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:188-201, 2018.

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A sensitive LC‐ESI‐Q‐TOF‐MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley

Cited by 36 publications

References 45 publications

Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard

Mass spectrometry‐derived systems biology technologies delineate the system's biochemical applications of siderophores

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A sensitive LC‐ESI‐Q‐TOF‐MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley

Cited by 36 publications

References 45 publications

Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a 13C4‐labeled internal standard

Mass spectrometry‐derived systems biology technologies delineate the system's biochemical applications of siderophores

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Accurate LC‐ESI‐MS/MS quantification of 2′‐deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C₄‐labeled internal standard