Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Tsednee, Munkhtsetseg; Yang, Shun‐Chung; Lee, Der-Chuen; Yeh, Kuo‐Chen

doi:10.1104/pp.114.241224

Cited by 68 publications

(34 citation statements)

References 66 publications

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“…Its apparent K d is 1.6 × 10 −11 M (Table 2) [82]. It transports zinc from roots to shoots and is also secreted from the roots in metal hyperaccumulator plants [83]. In this case, instead of being a chelating agent for uptake of a metal, it is secreted to serve as a chelating agent for the excess zinc in order to avoid further uptake.…”

Section: The Coordination Of Zinc In the “Free” Zinc Poolmentioning

confidence: 99%

The biological inorganic chemistry of zinc ions

Krężel

Maret

2016

Archives of Biochemistry and Biophysics

581

356

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The solution and complexation chemistry of zinc ions is the basis for zinc biology. In living organisms, zinc is redox-inert and has only one valence state: Zn(II). Its coordination environment in proteins is limited by oxygen, nitrogen, and sulfur donors from the side chains of a few amino acids. In an estimated 10% of all human proteins, zinc has a catalytic or structural function and remains bound during the lifetime of the protein. However, in other proteins zinc ions bind reversibly with dissociation and association rates commensurate with the requirements in regulation, transport, transfer, sensing, signalling, and storage. In contrast to the extensive knowledge about zinc proteins, the coordination chemistry of the “mobile” zinc ions in these processes, i.e. when not bound to proteins, is virtually unexplored and the mechanisms of ligand exchange are poorly understood. Knowledge of the biological inorganic chemistry of zinc ions is essential for understanding its cellular biology and for designing complexes that deliver zinc to proteins and chelating agents that remove zinc from proteins, for detecting zinc ion species by qualitative and quantitative analysis, and for proper planning and execution of experiments involving zinc ions and nanoparticles such as zinc oxide (ZnO). In most investigations, reference is made to zinc or Zn2+ without full appreciation of how biological zinc ions are buffered and how the d-block cation Zn2+ differs from s-block cations such as Ca2+ with regard to significantly higher affinity for ligands, preference for the donor atoms of ligands, and coordination dynamics. Zinc needs to be tightly controlled. The interaction with low molecular weight ligands such as water and inorganic and organic anions is highly relevant to its biology but in contrast to its coordination in proteins has not been discussed in the biochemical literature. From the discussion in this article, it is becoming evident that zinc ion speciation is important in zinc biochemistry and for biological recognition as a variety of low molecular weight zinc complexes have already been implicated in biological processes, e.g. with ATP, glutathione, citrate, ethylenediaminedisuccinic acid, nicotianamine, or bacillithiol.

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Section: The Coordination Of Zinc In the “Free” Zinc Poolmentioning

confidence: 99%

The biological inorganic chemistry of zinc ions

Krężel

Maret

2016

Archives of Biochemistry and Biophysics

581

356

View full text Add to dashboard Cite

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“…Enhanced metal uptake by these species does not seem to be related to specific phenomena, but to the enhancement of processes common with non-accumulators. They include a highly developed root system, enhanced transport activity at the soil-root interface, and possibly enhanced release (or release of different types) of organic ligands (Wenzel et al, 2003;Li et al, 2013;Tsednee et al, 2014). Arabidopsis halleri is a model of Zn, Cd hyperaccumulating plant, and it has been intensively studied for the genetics and physiology of metal tolerance and hyperaccumulation (Roosens et al, 2008;Sarret et al, 2009;Huguet et al, 2012;Verbruggen et al, 2013;Isaure et al, 2015;Meyer et al, 2015).…”

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confidence: 99%

Fate of cadmium in the rhizosphere of Arabidopsis halleri grown in a contaminated dredged sediment

Huguet

Isaure

Bert

et al. 2015

Science of The Total Environment

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In regions impacted by mining and smelting activities, dredged sediments are often contaminated with metals. Phytotechnologies could be used for their management, but more knowledge on the speciation of metals in the sediment and on their fate after colonization by plant roots is needed. This work was focused on a dredged sediment from the Scarpe river (North of France), contaminated with Zn and Cd. Zn, Cd hyperaccumulating plants Arabidopsis halleri from metallicolous and non-metallicolous origin were grown on the sediment for five months in a pot experiment. The nature and extent of the modifications in Cd speciation with or without plant were determined by electron microscopy, micro X-ray fluorescence and bulk and micro X-ray absorption spectroscopy. In addition, changes in Cd exchangeable and bioavailable pools were evaluated, and Cd content in leachates was measured. Finally, Cd plant uptake and plant growth parameters were monitored. In the original sediment, Cd was present as a mixed Zn, Cd, Fe sulfide. After five months, although pots still contained reduced sulfur, Cd-bearing sulfides were totally oxidized in vegetated pots, whereas a minor fraction (8%) was still present in non-vegetated ones. Secondary species included Cd bound to O-containing groups of organic matter and Cd phosphates. Cd exchangeability and bioavailability were relatively low and did not increase during changes in Cd speciation, suggesting that Cd released by sulfide oxidation was readily taken up with strong interactions with organic matter and phosphate ligands. Thus, the composition of the sediment, the oxic conditions and the rhizospheric activity (regardless of the plant origin) created favorable conditions for Cd stabilization. However, it should be kept in mind that returning to anoxic conditions may change Cd speciation, so the species formed cannot be considered as stable on the long term.

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“…The roles of transporters (Nouet et al, 2015) and chelating agents, such as nicotianamine, also were investigated recently (Deinlein et al, 2012;Tsednee et al, 2014). To some extent, the role of rhizosphere microorganisms in hyperaccumulation also has been identified (Farinati et al, 2009).…”

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confidence: 99%

Photosynthetic Efficiency as Bioindicator of Environmental Pressure in A. halleri

et al. 2017

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In earlier ecophysiological studies that were conducted on Arabidopsis halleri plants, scientists focused on the mechanisms of Cd and Zn hyperaccumulation but did not take into consideration the environmental factors that can significantly affect the physiological responses of plants in situ. In this study, we investigated A. halleri that was growing on two nonmetalliferous and three metalliferous sites, which were characterized by different environmental conditions. We compared these populations in order to find differences within the metallicolous and nonmetallicolous groups that have not yet been investigated. The concentrations of several elements in the plant and soil samples also were investigated. To our knowledge, the concentration and fluorescence of chlorophyll were measured for A. halleri in situ for the first time. Our study confirmed the hyperaccumulation of Cd and Zn for each metallicolous population. For the metallicolous populations, the inhibition of parameters that describe the efficiency of the photosynthetic apparatus with increasing accumulations of heavy metals in the shoots also was observed. It was found that the nonmetallicolous plant populations from the summit of Ciemniak Mountain had larger antenna dimensions and chlorophyll content but a lower percentage of active reaction centers. To our knowledge, in this study, the internal high physiological diversity within the populations that inhabit metalliferous and nonmetalliferous sites is presented for the first time.

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Root-Secreted Nicotianamine from Arabidopsis halleri Facilitates Zinc Hypertolerance by Regulating Zinc Bioavailability

Cited by 68 publications

References 66 publications

The biological inorganic chemistry of zinc ions

The biological inorganic chemistry of zinc ions

Fate of cadmium in the rhizosphere of Arabidopsis halleri grown in a contaminated dredged sediment

Photosynthetic Efficiency as Bioindicator of Environmental Pressure in A. halleri

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