A sensitive immunochromatographic assay using gold nanoparticles for the semiquantitative detection of prostate-specific antigen in serum

Nagatani, Naoki; Yuhi, Teruko; Chikae, Miyuki; Kerman, Kağan; Endo, Tatsuro; Kobori, Yoshitomo; Takata, Masayuki; Konaka, Hiroyuki; Namiki, Mikio; Ushijima, Hiromi; Takamura, Yuzuru; Tamiya, Eiichi

doi:10.1007/bf02697262

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…Colloidal gold nanoparticles often serve as signal label of ITS for detection of varieties of analytes due to its unique optical properties and remarkable physical-chemical stability. Many gold-based ITS have been applied for qualitative and semiquantitative detection of protein biomarkers. − In addition, significant attempts still need to be made to improve the sensitivity of ITS by introducing various labels ,− including quantum dots, , colored latex particles, and up-converting phosphors. − These nanomaterials exhibit higher sensitivity and broader response range than colloidal gold based conventional ITS. Nonetheless, the increased sensitivity of these nanomaterials generates several drawbacks.…”

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confidence: 99%

Detection of p53 Protein Based on Mesoporous Pt–Pd Nanoparticles with Enhanced Peroxidase-like Catalysis

Jiang

Yang²,

et al. 2016

ACS Sens.

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We reported a sensitive immunochromatographic test strip based on mesoporous Pt−Pd bimetal nanoparticles (NPs) with greatly enhanced peroxidase-like catalysis for detection of p53 visually and quantitatively. The principle of this assay is relied on the measurements of visual color intensity produced by peroxidase reaction with 3,3′,5,5′tetramethylbenzidine (TMB) solution. Owing to the strong peroxidase-like activity of Pt−Pd NPs, it exhibited strong visual color in less than 30 s in low concentration range of target analytes. Quantitative measurement of p53 was performed with a hand-held test strip reader, which yields a detection limit of 0.05 ng mL −1 with the linear range of 0.1−10 ng mL −1 . Although the feasibility was demonstrated using p53 as a model analyte, this approach could be easily extended for detection of other protein biomarkers.

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confidence: 99%

Detection of p53 Protein Based on Mesoporous Pt–Pd Nanoparticles with Enhanced Peroxidase-like Catalysis

Jiang

Yang²,

et al. 2016

ACS Sens.

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“…We began the fabrication of our sensors through physical adsorption of polyethylene glycol (PEG) onto SPCE. Concurrently, the signal amplification probes were prepared by mixing polyclonal secondary anti-ENO1 with 33.0 nm diameter AuNPs, thereby providing 151 nm AuNP congregates sensitized with secondary anti-ENO1 antibody molecules. , After sequential incubation with sample ENO1, the secondary anti-ENO1-tagged AuNP congregate bioprobes formed immunocomplexes on the primary monoclonal anti-ENO1-modified SPCE surface. Subsequent analysis using square wave voltammetry (SWV) revealed that this electrochemical immunosensor allowed the quantification of ENO1 to levels as low as 2.38 pg/mL.…”

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confidence: 99%

Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor

et al. 2010

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The development of rapid and sensitive methods for the detection of immunogenic tumor-associated antigen is important not only for understanding their roles in cancer immunology but also for the development of clinical diagnostics. Alpha-enolase (ENO1), a p48 molecule, is widely distributed in a variety of tissues, whereas gamma-enolase (ENO2) and beta-enolase (ENO3) are found exclusively in neuron/neuroendocrine and muscle tissues, respectively. Because ENO1 has been correlated with small cell lung cancer, nonsmall cell lung cancer, and head and neck cancer, it can be used as a potential diagnostic marker for lung cancer. In this study, we developed a simple, yet novel and sensitive, electrochemical sandwich immunosensor for the detection of ENO1; it operates through physisorption of anti-ENO1 monoclonal antibody on polyethylene glycol-modified disposable screen-printed electrode as the detection platform, with polyclonal secondary anti-ENO1-tagged, gold nanoparticle (AuNP) congregates as electrochemical signal probes. The immunorecognition of the sample ENO1 by the congregated AuNP@antibody occurred on the surface of the electrodes; the electrochemical signal from the bound AuNP congregates was obtained after oxidizing them in 0.1 M HCl at 1.2 V for 120 s, followed by the reduction of AuCl(4-) in square wave voltammetry (SWV) mode. The resulting sigmoidally shaped dose-response curves possessed a linear dynamic working range from 10(-8) to 10(-12) g/mL. This AuNP congregate-based assay provides an amplification approach for detecting ENO1 at trace levels, leading to a detection limit as low as 11.9 fg (equivalent to 5 microL of a 2.38 pg/mL solution).

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“…Strip based ICG is very attractive in this context. Fortunately, during the last decade, few ICG test strips for screening of cancer have been commercially developed, such as for prostate cancer in the case of sensing prostate specific antigen (Nagatani et al, 2006;Omidfar et al, 2012b). EBV-specific VCA-IgA and other antibodies are measured using the ELISA assay in large screening programs (Cao et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

A new gold nanoparticle based rapid immunochromatographic assay for screening EBV-VCA specific IgA in nasopharyngeal carcinomas

Goudarzi¹,

Ahmadi²,

Farhadi³

et al. 2015

JAB

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Gold nanoparticleNasopharyngeal carcinoma Immunochromatography Screening test a b s t r a c t Large screening programs of nasopharyngeal carcinoma (NPC) are still challenging due to the scarcity of the resources in higher prevalence parts of the world. In the present study, the development of a new low cost immunochromatography (ICG) strip test for screening of nasopharyngeal carcinoma is reported. A conjugate of gold nanoparticle-anti viral capsid antigen (VCA) immunoglobulin A (IgA) antibody was used as the detection probe. The test is based on a non-competitive immunoassay for rapid and reliable detection the serum specific IgA of Epstein-Barr virus (EBV) VCA. Patient serum samples were analyzed with the strip and compared with enzyme-linked immunosorbent assay (ELISA) and pathological evaluation.The obtained results from the ICG strips were well correlated with the two reference methods. An evaluation of stability, sensitivity and specificity suggested the strip suitable for NPC screening programs in high-risk peoples and also for the recurrence monitoring.

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A sensitive immunochromatographic assay using gold nanoparticles for the semiquantitative detection of prostate-specific antigen in serum

Cited by 11 publications

References 23 publications

Detection of p53 Protein Based on Mesoporous Pt–Pd Nanoparticles with Enhanced Peroxidase-like Catalysis

Detection of p53 Protein Based on Mesoporous Pt–Pd Nanoparticles with Enhanced Peroxidase-like Catalysis

Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor

A new gold nanoparticle based rapid immunochromatographic assay for screening EBV-VCA specific IgA in nasopharyngeal carcinomas

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