A sensitive Enzyme-Linked Immunosorbent Assay (ELISA) against the major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens

Luka, János; Chase, Robert C.; Pearson, Gary R.

doi:10.1016/0022-1759(84)90093-0

Cited by 83 publications

(53 citation statements)

References 24 publications

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“…The 28-kDa E3NA ELISA is thus both sensitive and specific. Finally, as would be necessary to validate the use of the 28-kDa EBNA polypeptide as an ELISA reagent, the overall pattern of ELISA determined anti-28-kDa EBNA titers was consistent with results obtained previously by others using ACIF (26) or the earlier ELISAs (27)(28)(29). Specifically, low EBNA titers were observed in the early stages of infectious mononucleosis, whereas nasopharyngeal carcinoma patients all had high EBNA titers and some Burkitt lymphoma patients had high EBNA titers, while others were in the normal range.…”

Section: Discussionsupporting

confidence: 76%

“…Previous reports of the use of ELISAs to measure anti-EBV antibody titers (27)(28)(29) earlier studies, the source of EBNA antigen was either total extracts (27) The sensitivity of the bacterial antigen ELISA is illustrated by the finding that nasopharyngeal carcinoma patient sera with ACIF titers between 1:320 and 1:640 gave ELISA titers between 1:50,000 and 1:100,000. However, the titers of individual serum samples measured by 28-kDa EBNA ELISA were not always directly comparable to those measured by ACIF, which may be a reflection of the presence in some human sera of antibodies against other EBNAs (6, 7) and against epitopes on the amino-terminal two-thirds of BamHI fragment K-encoded EBNA.…”

Section: Discussionmentioning

confidence: 97%

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Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man.

Milman¹,

Scott²,

Cho³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 97%

Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man.

Milman¹,

Scott²,

Cho³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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“…Some investigators found EIA to be more sensitive than IFA, particularly the anticomplement immunofluorescence technique (22); others found the EIA technology to be as sensitive as IFA or even more sensitive than EIA (23,27). Already in the mid-1980s the results of assays performed with the first generations of EIAs correlated nicely with those of IFA with purified VCA and EBNA proteins as antigens (9).…”

Section: The Spectrum Of Serological Methodsmentioning

confidence: 99%

Routine Epstein-Barr Virus Diagnostics from the Laboratory Perspective: Still Challenging after 35 Years

2004

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“…Os testes de ELISA foram interpretados seguindo as orientações do fabricante, em unidades arbitrárias (AU/ ml), as quais foram obtidas de curvas padronizadas de amostras de soro, com titulação conhecida de anticorpos específicos anti-EBV 23 .…”

Section: Interpretação Dos Resultadosunclassified

“…A detecção da IgG anti-VCA, através da técnica de ELISA, também evidenciou diferença estatisticamente significante entre as crianças com AIDS e as do grupo controle (p < 0,0001). Esta técnica tem sido referida em alguns trabalhos como sensível e específica para a pesquisa de anticorpos contra antígenos virais do EBV 23,32 .…”

Section: Discussionunclassified

Epstein-Barr virus infection in children with AIDS

Carvalho¹,

Gandolfi²,

Tonelli³

1998

J Pediatr (Rio J)

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ResumoObjetivo: A associação das infecções por HIV e vírus EpsteinBarr tem sido relatada em diversos estudos, especialmente em crianças que apresentam pneumonia intersticial linfóide. Analisouse a incidência da infecção pelo vírus Epstein-Barr em crianças com AIDS, naquelas com AIDS com e sem pneumonia intersticial linfóide, comparando-se com crianças não infectadas pelo HIV.Métodos AbstractObjective: The association of the HIV and Epstein-Barr virus infections has been reported in several studies, specially in children that present lymphocytic interstitial pneumonitis. The analysis of the incidence of Epstein-Barr virus infection was performed in children with AIDS, in those with AIDS with and without lymphocytic interstitial pneumonitis, comparing to children without HIV infection.Methods: A cross-sectional study was performed with 60 children with AIDS and 54 children not infected by HIV, matched by sex and age, in the Hospital Infantil Joana de Gusmão in Florianópolis city, Santa Catarina State, Brazil, between June 1994 and June 1995. The selection of the cases followed the order of the consultation. The detection of antibodies against Epstein-Barr capsid antigen was performed by standard indirect immunofluorescence techniques and ELISA, and antibodies against nuclear antigen only by ELISA. The stage of the infection was defined according to serologic responses.Results: Lymphadenopathy occurred in 59 cases (98.3%), hepatomegaly in 51 (85.0%), splenomegaly in 46 (76.7%), recurrent bronchopneumonia in 41 (68.3%). Lymphocytic interstitial pneumonitis was observed in 21 cases (35.0%), 90.5% of which showed positive serologic reactions to EBV. The geometric mean of the IgG against capsid antigen titers was 1:439.5 in the cases group and 1:42.8 in the controls. The IgM against capsid antigen by indirect immunofluorescence techniques was positive in 16 (26.7%) of the 60 cases and only in one of the 53 controls (1.9%) (p=0.0002). The primary Epstein-Barr virus infection was evidenced with greater frequency in the children with AIDS (p<0.0001), as well as the reactivated infection (p=0.0745).Conclusions: The incidence of primary and reactivated EpsteinBarr virus infection was more frequent in the patients with AIDS. The primary and reactivated Epstein-Barr virus infection was observed in almost all of the patients with diagnosis of lymphocytic interstitial pneumonitis (90.5%).J. pediatr. (Rio J.). 1998; 74(2):125-134 IntroduçãoAs similaridades clínicas decorrentes da infecção pelo vírus da imunodeficiência humana (HIV) e pelo vírus Epstein-Barr (EBV), especialmente relacionadas às manifestações envolvendo o sistema linfático, além de semelhanças no comprometimento do sistema imunológico,

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A sensitive Enzyme-Linked Immunosorbent Assay (ELISA) against the major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens

Cited by 83 publications

References 24 publications

Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man.

Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man.

Routine Epstein-Barr Virus Diagnostics from the Laboratory Perspective: Still Challenging after 35 Years

Epstein-Barr virus infection in children with AIDS

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