A sensitive enzymatic assay for the determination of sucrose in serum and urine

Microtubule assembly in meiotic extract requires glycogen

“…We added thio-NADP as a rapid test of glucose metabolism in C-HSS. Reduction of this compound to thio-NADPH is irreversible, depends on glucose metabolism (via glucose-6-phosphate dehydrogenase), and is readily observed by measuring its absorbance at 405 nm (Seimiya et al , 2004). C-HSS did not generate a 405-nm absorbance, while C-HSS plus glycogen did.…”

Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC–MS/MS for the studies of intestinal and upper digestive tract permeability

“…Most often used are the enzymatic methods. However, estimation of the concentration of lactulose and mannitol is not always accurate [17][18][19][20][21]. Gas chromatography requires evaporation and derivatization of the analytes present in studied samples prior injection.…”

“…The lactulose/mannitol test assumes that these two compounds are treated identically in all individual and physiological aspects [15,16]. Sucrose permeability test is commonly accepted as a marker of upper digestive tract (stomach and duodenum) mucosal barrier damage [17,18].…”

Determination of sucrose in equine serum using liquid chromatography–mass spectrometry (LC/MS)

“…Gas chromatography/mass spectrometry (GC/MS), high performance liquid chromatography/mass spectrometry (LC/MS), enzymatic assays and colourimetric analysis may be used to quantify carbohydrates in complex matrices [5][6][7][8][9][10], including serum and urine. Serum samples are readily obtained and, by collection of serial samples, can provide a dynamic picture of sucrose absorption kinetics, whereas urine samples are less readily collected and can accumulate in the bladder for a variable time before collection.…”