A sensitive electrochemiluminescence cytosensor for quantitative evaluation of epidermal growth factor receptor expressed on cell surfaces

Tang, Yanjuan; Zhang, Shaolian; Wen, Qingqing; Huang, Hongxing; Yang, Peihui

doi:10.1016/j.aca.2015.04.008

Cited by 24 publications

(10 citation statements)

References 21 publications

(28 reference statements)

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“…However, single-cell analysis suffers from some challenges, such as the small size of a single cell and the low expression levels of biomolecules at single-cell levels, which limits the development of single-cell analysis. Biological investigations of conventional methods reflect the average results from large cell populations, which cannot illustrate the actual properties of an individual cell. , Thus, there is an urgent need to develop a novel ultrasensitive assay to effectively analyze the single cell and study the individual cellular function.…”

Section: Introductionmentioning

confidence: 99%

“…Carbon quantum dots (CQDs), as novel types of carbon nanomaterials, have been applied extensively as ECL probes in biological sensors because of their ease of preparation, chemical inertness, low cytotoxicity, ease of labeling, and good biocompatibility. , However, the low luminescence efficiency of water-soluble CQDs limited their application in single-cell analysis, so that it is essential to enhance the ECL intensity of CQDs for the purpose of fabricating an ultrasensitive ECL platform, which could obtain a credible signal for single-cell analysis. Nanomaterials, such as graphene, metal nanoparticles, − and magnetic beads (MBs), combined with a signal amplification strategy, have been widely used as biosensors because of their good biocompatibility and conductivity, which can accelerate signal transduction and amplify the signal . In our previous work, the concanavalin A functionalized Au@Luminol ECL probe was labeled on MCF-7 cells for the detection of mannose, attributed to the good conductivity and catalytic properties of gold nanoparticles (AuNPs) for signal amplification .…”

Section: Introductionmentioning

confidence: 99%

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Novel Single-Cell Analysis Platform Based on a Solid-State Zinc-Coadsorbed Carbon Quantum Dots Electrochemiluminescence Probe for the Evaluation of CD44 Expression on Breast Cancer Cells

Qiu

Zhou

Xiao

et al. 2017

ACS Appl. Mater. Interfaces

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A novel single-cell analysis platform was fabricated using solid-state zinc-coadsorbed carbon quantum dot (ZnCQDs) nanocomposites as an electrochemiluminescence (ECL) probe for the detection of breast cancer cells and evaluation of the CD44 expression level. Solid-state ZnCQDs nanocomposite probes were constructed through the attachment of ZnCQDs to gold nanoparticles and then the loading of magnetic beads to amplify the ECL signal, exhibiting a remarkable 120-fold enhancement of the ECL intensity. Hyaluronic acid (HA)-functionalized solid-state probes were used to label a single breast cancer cell by the specific recognition of HA with CD44 on the cell surface, revealing more stable, sensitive, and effective tagging in comparison with the water-soluble CQDs. This strategy exhibited a good analytical performance for the analysis of MDA-MB-231 and MCF-7 single cells with linear range from 1 to 18 and from 1 to 12 cells, respectively. Furthermore, this single-cell analysis platform was used for evaluation of the CD44 expression level of these two cell lines, in which the MDA-MB-231 cells revealed a 2.8-5.2-fold higher CD44 expression level. A total of 20 single cells were analyzed individually, and the distributions of the ECL intensity revealed larger variations, indicating the high cellular heterogeneity of the CD44 expression level on the same cell line. The as-proposed single-cell analysis platform might provide a novel protocol to effectively study the individual cellular function and cellular heterogeneity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel Single-Cell Analysis Platform Based on a Solid-State Zinc-Coadsorbed Carbon Quantum Dots Electrochemiluminescence Probe for the Evaluation of CD44 Expression on Breast Cancer Cells

Qiu

Zhou

Xiao

et al. 2017

ACS Appl. Mater. Interfaces

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“…EGFR is a transmembrane protein and its overexpression is associated with a number of cancers. In particular, EGFR has been observed in ≈33% of breast cancers, and overexpressed in MCF‐7 breast cancer cells with a concentration of ≈10 −7 mol L −1 …”

Section: Resultsmentioning

confidence: 99%

Targeted Live Cell Raman Imaging and Visualization of Cancer Biomarkers with Thermal‐Stimuli Responsive Imprinted Nanoprobes

Zhang

Qin

Tan

et al. 2018

Part & Part Syst Charact

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Precise visualization of cancer biomarkers within live cells is significantly vital in oncology that requires techniques with high specificity and selectivity. Herein, a new imprinted plasmonic nanoprobe with thermosensitivity is fabricated, which features multifunctional roles as an “artificial antibody” for the active targeting of cancer biomarkers triggered by temperature and can also achieve their in situ Raman detection and imaging within living cells. A thiol‐containing poly(N‐isopropylacrylamide) linear polymer is first synthesized using reversible addition–fragmentation chain transfer polymerization followed by further reduction with NaBH4 to produce free thiol groups at one end. Then, plasmonic nanoprobes are constructed by creating protein‐imprinted poly(N‐isopropylacrylamide) self‐assembled monolayers on the surface of gold nanorods. High specificity and selectivity are demonstrated for the target protein, with an extracellular Raman detection limit of 10−14 mol L−1. Thermally responsive behavior is achieved by modulating the external temperature. The plasmonic properties enable in situ live cell intracellular Raman detection and imaging of endogenous cancer biomarker epidermal growth factor receptor. This technique offers significant promise for visualizing cancer biomarkers and monitoring molecular mechanisms both in vitro and in vivo, which are significant in clinical diagnosis and medical theranostics.

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“…To enhance the sensitivity of ECL methods, several interesting strategies have been proposed by employing the nanomaterials as the ECL enhancers, the layer-by-layer technique for increasing the dose of QDs on the electrode surface, and the nanohybrids for signal enhancement. For example, MBs were utilized to carry CdS QDs for the development of ECL cytosensor based on the interaction between the epidermal growth factor and the epidermal growth factor receptor on cell surface [242]. Highly branched dendrimer can be used to load large numbers of QDs for improving the density of QDs and enhancing the ECL signal [243].…”

Section: Electrochemiluminescence Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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A sensitive electrochemiluminescence cytosensor for quantitative evaluation of epidermal growth factor receptor expressed on cell surfaces

Cited by 24 publications

References 21 publications

Novel Single-Cell Analysis Platform Based on a Solid-State Zinc-Coadsorbed Carbon Quantum Dots Electrochemiluminescence Probe for the Evaluation of CD44 Expression on Breast Cancer Cells

Novel Single-Cell Analysis Platform Based on a Solid-State Zinc-Coadsorbed Carbon Quantum Dots Electrochemiluminescence Probe for the Evaluation of CD44 Expression on Breast Cancer Cells

Targeted Live Cell Raman Imaging and Visualization of Cancer Biomarkers with Thermal‐Stimuli Responsive Imprinted Nanoprobes

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

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