A sensitive assay based on specific aptamer binding for the detection of Salmonella enterica serovar Typhimurium in milk samples by microchip capillary electrophoresis

Zhang, Yan; Luo, Feifei; Zhang, Yating; Zhu, Ling; Li, Yang; Zhao, Shouxun; He, Pingang; Wang, Qingjiang

doi:10.1016/j.chroma.2017.12.054

Cited by 43 publications

(14 citation statements)

References 32 publications

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“…The aptamer MCE method was used for the detection of several proteins , immunoglobulin E , drugs , or bacteria . Figure represents an example of the use of aptamer MCE for the detection of antibiotics in a food .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

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Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

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Capillary electrophoresis-based immunoassay and aptamer assay: A reviewOver the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.Abbreviations: Ag, antigen; CEIA, CE immunoassay; MCE, microchip CE; SELEX, systematic evolution of ligands by exponential enrichment and they are produced by the immune system of living organisms. Ag that start the immunological response are also called immunogens. These Ab-Ag complexes are stabilized by dipole-dipole, van der Waals or hydrophobic interactions and hydrogen bonds [5,6].The most often used immunoassays are ELISA with Ab or Ag immobilized onto solid support (plates, glass fibers, or plastic tubes), immunofluorescence assay, fluorescence polarization immunoassay, luminescence immunoassay, microparticle enzyme immunoassay, and biosensors. The major disadvantages of immunoassays are the tedious process, time-consuming reactions, and problems with reproducibility and nonspecific interactions [6].CE is a powerful analytical method for the separation of a wide range of molecules. The coupling of CE and immunoassay in the new CE immunoassay (CEIA) technique provided both separation power and specificity of interactions. Other advantages are the possibility of multianalyte analyses, automation, low sample consumption, high speed, flexibility, a lower amount of false-positive results, and also the possibility of connection with highly sensitive detectors [5].In CEIA, the Fab fragments of Ab containing Ag-specific regions are more often used than the whole monoclonal and Color online: See article online to view Figs. 4 and 7 in color.

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Section: Aptamer‐based Affinity Cementioning

confidence: 99%

“…Figure represents an example of the use of aptamer MCE for the detection of antibiotics in a food . A summary of the applications of aptamer MCE is listed in Table .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

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“…These high affinity screened oligomers are then aptly labelled "Aptamer" for that particular target. Over the years a multitude of SELEX variants have been developed, viz., cell-SELEX, 35 capillary electrophoresis based SELEX, 40,41 microuidics-SELEX, 42,43 FACS based SELEX, 44 microtitre plate-SELEX, 45,46 magnetic bead SELEX, 47 and in vivo SELEX. 48,49 Each method has been developed considering the nal application of the aptamer with respect to the target of interest and various researchers have successfully adopted several modications.…”

Section: Introductionmentioning

confidence: 99%

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Kaur

Shorie

2019

Nanoscale Adv.

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“…Due to its high separation efficiency, easy operation, and rapid quantification, there has been great interest in using MCE for the detection of biological species, e.g. DNA, amino acids, proteins, and bacteria .…”

Section: Introductionmentioning

confidence: 99%

Selective detection of cytochrome C by microchip electrophoresis based on an aptamer strategy

Zhu

Luo

et al. 2019

Electrophoresis

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The release of cytochrome C (Cyt C) plays an important role in apoptosis. In this study, selective and sensitive detection of Cyt C based on an aptamer strategy coupled with MCE was developed. Following the binding of a specific aptamer to Cyt C, the aptamer exhibited an irregular state, reducing the binding affinity of a fluorescent probe, and thus preventing the aptamer‐Cyt C complexes from detection within the MCE. The height of the detection peak of the residual aptamer linearly decreased, and therefore, the difference in peak height of residual aptamer compared to that of the initial aptamer was used to quantify the captured protein concentration. Experimental conditions such as incubation time, pH, temperature, and ionic strength were optimized. A measurement of Cyt C concentration by MCE was achieved within 135 s, with a limit of detection as low as 0.4 nM. The proposed method has high selectivity and good stability for the detection of Cyt C. The experimental results demonstrate that this method is quick, consumes only a small quantity of sample, is highly selectivity and exhibits high sensitivity.

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A sensitive assay based on specific aptamer binding for the detection of Salmonella enterica serovar Typhimurium in milk samples by microchip capillary electrophoresis

Cited by 43 publications

References 32 publications

Capillary electrophoresis–based immunoassay and aptamer assay: A review

Capillary electrophoresis–based immunoassay and aptamer assay: A review

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Selective detection of cytochrome C by microchip electrophoresis based on an aptamer strategy

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