A sensitive and specific lateral flow assay for rapid detection of antibodies against glycoprotein B of Aujeszky's disease virus

Vrublevskaya, V. V.; Afanasyev, V. N.; Grinevich, Andrey A.; Skarga, Y. Y.; Gladyshev, P. P.; Ibragimova, S.A.; Krylsky, Dmitry V.; Dezhurov, Sergey V.; Morenkov, O. S.

doi:10.1016/j.jviromet.2017.09.012

Cited by 14 publications

(8 citation statements)

References 16 publications

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“…These diagnostic methods use antibodies as either capture/detection elements or the assay's primary targets. Antibodies conjugated into nanoparticles have proven to be excellent biorecognition elements due to stability, biocompatibility, and its sensitivity to the target antigens even at lower concentrations ( 16 ). As targets, the presence of certain antibodies in animal sera, blood, and urine can also be strong indicators of pathogen exposure and infection ( 17 ).…”

Section: Immuno-based Methodsmentioning

confidence: 99%

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Manhas

Quintela

2021

Front. Vet. Sci.

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Nanotechnology has gained prominence over the recent years in multiple research and application fields, including infectious diseases in healthcare, agriculture, and veterinary science. It remains an attractive and viable option for preventing, diagnosing, and treating diseases in animals and humans. The apparent efficiency of nanomaterials is due to their unique physicochemical properties and biocompatibility. With the persistence of pathogens and toxins in the poultry and livestock industries, rapid diagnostic tools are of utmost importance. Though there are many promising nanomaterials-based diagnostic tests specific to animal disease-causing agents, many have not achieved balanced sensitivity, specificity, reproducibility, and cost-effectiveness. This mini-review explores several types of nanomaterials, which provided enhancement on the sensitivity and specificity of recently reported diagnostic tools related to animal diseases. Recommendations are also provided to facilitate more targeted animal populations into the development of future diagnostic tools specifically for emerging and re-emerging animal diseases posing zoonotic risks.

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Section: Immuno-based Methodsmentioning

confidence: 99%

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Manhas

Quintela

2021

Front. Vet. Sci.

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“…The seriousness of this situation is also emphasised by other researchers. In particular, Vrublevskaya et al (2017a) addressed the difficulties in developing a vaccine against the ADV. Describing the results of their studies, the authors wrote: "A direct sidestream assay of double antibodies (DDA-gB-LFA) was developed to detect antibodies against the glycoprotein B (gB) of ADV in porcine serum.…”

Section: Introductionmentioning

confidence: 99%

Epizootic situation of Aujeszky’s disease within the territory of the Republic of Kazakhstan

Koshemetov¹,

Kondibayeva²,

Mussoyev

et al. 2021

Open Vet J.

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Background: The relevance of the study is determined by the danger that this disease poses to all types of domestic animals in the Republic of Kazakhstan, as well as the significant damage caused by this situation to the entire agriculture of the Republic of Kazakhstan. Timely and competent analysis of the epizootic situation for this disease in the country will reliably determine the development tendencies of this condition and predetermine a set of measures that should be taken to combat this animal disease in agricultural regions of the Republic of Kazakhstan. Aim: This study examines the development of the epizootic situation of Aujeszky’s disease within the territory of the Republic of Kazakhstan. Methods: To achieve the set research goal, a combination of quantitative and qualitative research methods are used. Results: The results of this research article can be of significant importance for livestock breeders in the Republic of Kazakhstan, who are working on raising the number of pigs and other types of domestic animals and are interested in a further increase in the number. Conclusion: From a practical standpoint, the significance of this study is conditioned by the possibility of searching and determining the main directions of the spread of the disease under consideration in agricultural lands and regions of the Republic of Kazakhstan, as well as taking a set of measures to eradicate such a disease and prevent its future occurrence in the country.

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“…On the contrary, LFIST, which utilizes the specific interaction between antibody and antigen in a sandwich format 21 or competitive format 22 , is rapid and easy to be used by non-professionals. LFISTs for the specific detection of bacteria 23 , viruses 24 , pesticides 25 , veterinary drugs 26 – 28 , toxins 29 , 30 and allergens 31 , 32 have been developed and tested. However, there is no available LFIST for detecting OP currently.…”

Section: Introductionmentioning

confidence: 99%

A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

Yang

Wang

et al. 2018

Sci Rep

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A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC50) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 µg/kg, and the limit detection (LOD) value were 0.43 and 0.42 µg/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intra-assay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.

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A sensitive and specific lateral flow assay for rapid detection of antibodies against glycoprotein B of Aujeszky's disease virus

Cited by 14 publications

References 16 publications

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Epizootic situation of Aujeszky’s disease within the territory of the Republic of Kazakhstan

A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

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