A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

Yang, Xing-Dong; Yang, Jifei; Wang, Yinbiao; Li, Lili; Sun, Zhongke; Yue, Zonghao; Tian, Fang; He, Le; Hu, Xiaofei

doi:10.1038/s41598-018-35080-5

Cited by 22 publications

(9 citation statements)

References 38 publications

(37 reference statements)

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“…As aforementioned, LFAs are used in a variety of applications with samples that present extremely different composition and characteristics (Table 1). 27 For example, clinical samples can be whole blood, 28,29 plasma, 30,31 serum, [32][33][34] sweat, 35,36 urine, 37,38 stool, 39,40 saliva, 41,42 cerebrospinal fluid 43,44 and nasal swabs, 45,46 while food matrices could be juices, 47,48 cereals, 49,50 meat, 51,52 vegetables, 53 and environmental (mostly water and soil) samples. [54][55][56][57] Although the sample pad provides a means to control the properties of the sample solution (see the following section), some complex matrixes may require pre-treatment before an aliquot can be added into the LFA strip.…”

Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…As shown in Figure 1, the test strip consisted of the sample pad, absorption pad, nitrocellulose (NC) membrane, and polyvinyl chloride (PVC) backing card, and its assembly was performed according to previous methods (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007;Yang et al, 2018;Yu et al, 2015). The coating antigen and the goat anti-mouse IgG were both dispensed onto the NC membrane to form the test line (T line) and control line (C line), and then the membrane was dried at 37°C for 2 h in an air oven.…”

Section: Assembly Of the Test Stripmentioning

confidence: 99%

A sensitive lateral flow immunoassay for the multiple residues of five adamantanes

Gao

Wang

et al. 2019

Food and Agricultural Immunology

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Adamantanes (ADAs) are tricyclic decane saturated derivatives, which are used in the protection against the influenza virus. However, their abuse in animal husbandry has affected human health. To help prevent drug resistance and protect food safety, a rapid, portable, sensitive, and reliable method for onsite detection of ADAs was developed. Herein, we have produced a broadspecificity anti-ADAs monoclonal antibody (mAb), the subclass of which is IgG 2b , and its affinity constant is 7.25 × 10 9 L/mol. From this, an antibody-based lateral flow immunoassay (LFIA) was developed for the rapid detection of five ADAs in food samples. When viewed with the naked eye, the LFIA had a visual detection limit of 0.1, 0.1, 1, 5, and 10 μg/kg and a cutoff value of 2.5, 2.5, 10, 100, and 100 μg/kg for somantadine, rimantadine, amantadine, 1-adamantanemethanol, 1-adamantyl methyl ketone respectively in chicken samples. The test results for the LFIA were very similar to that seen when using an enzyme linked immunosorbent assay (ELISA). Therefore, this paper-based LFIA provides a useful tool for rapid, onsite detection of ADAs in biological samples.

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“…Therefore it is essential to develop a rapid, simple and effective device for NIT detection in the field. Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes . Its user‐friendly format, simple analysis and low cost make it commonly used in real‐time detection of veterinary drug residues .…”

Section: Introductionmentioning

confidence: 99%

“…Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes. [19][20][21][22][23] Its user-friendly format, simple analysis and low cost make it commonly used in real-time detection of veterinary drug residues. [24][25][26][27][28] To the best of our knowledge, only one paper has reported an application for NIT detection based on the immunological method.…”

Section: Introductionmentioning

confidence: 99%

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

Yang

et al. 2020

J Sci Food Agric

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BACKGROUND Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food‐producing cattle and sheep. NIT has a long half‐life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk. RESULTS The affinity constant of the anti‐NIT mAb was 2.93 × 1010 and the anti‐NIT mAb had almost no cross‐reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL−1 by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50) of the immunoassay strip was calculated to be 5.716 ng mL−1 and the limit of detection (LOD) was 1.146 ng mL−1. Intra‐assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter‐assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%. CONCLUSION The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. © 2019 Society of Chemical Industry

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A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

Cited by 22 publications

References 38 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

A sensitive lateral flow immunoassay for the multiple residues of five adamantanes

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

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