A sensitive and specific fluorimetric method for the determination of noradrenalin and adrenalin in human plasma

Renzini, V.; Brunori, Claudia; Valori, C

doi:10.1016/0009-8981(70)90249-4

Cited by 165 publications

(58 citation statements)

References 4 publications

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“…Plasma and CSF catecholamines were measured by the fluorimetric method of Renzini et al, 10 as modified by Miura et al 11 Cerebrospinal fluid NE was measured by the method of Peuler and Johnson in one patient. 12 Plasma NE measured by the Renzini method was identical to that of the radioenzymatic method in this patient.…”

Section: Methodsmentioning

confidence: 99%

Raised cerebrospinal fluid norepinephrine in some patients with primary hypertension.

Eide¹,

Kolloch²,

Quattro³

et al. 1979

Hypertension

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SUMMARY To test whether central neurogenic factors participate in blood pressure elevation in primary hypertension, we studied the concentrations of: norepinephrine, epinephrine and dopamine-beta-hydroxylase (DBH) in cerebrospinal fluid (CSF); and norepinephrine, epinephrine, DBH and plasma renin activity (PRA) in plasma of 22 subjects (seven with primary hypertension, 11 normotensive patients with non-systemic neurological disorders, and four with secondary hypertension). Plasma and CSF norepinephrine (NE) were increased in primary hypertensives compared to normotensives. Cerebrospinal fluid norepinephrine was related to diastolic blood pressure, and systolic blood pressure when normotensive and primary hypertensives were taken together. The CSF norepinephrine of primary hypertensive patients was correlated with natural log PRA. The CSF norepinephrine was correlated inversely with age in primary hypertensive patients but not in the normotensive subjects. The low CSF norepinephrine and epinephrine, despite markedly increased plasma NE and epinephrine, in two patients with pbeochromocytoma, indicate a blood-brain barrier for these neurohormones. The observations support the view that the central sympathetic nervous system is involved in the pathogenesis of primary hypertension, particularly in younger patients. 1 " Hyperactivity of the central sympathetic nervous system might raise plasma norepinephrine and lead to the development of hypertension. Because of an efficient blood-brain barrier to norepinephrine (NE) it seems possible to evaluate central nervous system NE release by measuring NE in cerebrospinal fluid (CSF). Therefore, in this study, we compared CSF NE concentrations from patients with primary hypertension with those from normotensive patients with various neurological disorders. We found increased NE in CSF and plasma from the hypertensive group, which suggests that central sympathetic hypertonicity is associated with raised peripheral sympathetic nerve tone and elevated blood pressure in younger patients with primary hypertension.

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Section: Methodsmentioning

confidence: 99%

Raised cerebrospinal fluid norepinephrine in some patients with primary hypertension.

Eide¹,

Kolloch²,

Quattro³

et al. 1979

Hypertension

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“…In the method described the only variable error to this point was the amount of alumina lost in transfer between the tubes and the columns and this was kept to a minimum by water vortexing. Renzini et al (1970) suggested the addition of di-sodium EDTA stabilised the eluate for 2 weeks at 4°. The addition was made but, as no indication was given as to the stability at room temperature ofthe solution, it was considered necessary to assay it as quickly as possible.…”

Section: Resultsmentioning

confidence: 99%

“…The method of analysis using the ferricyanide oxidation to noradrenolutine was based on procedures outlined by Renzini et al (1970) and Wood and Mainwadng-Burton (1975). 2-mercaptoethanol was used as the stabilising agent under alkaline conditions because it gave low blanks, was cheaper than related mercapto-stabilising agents and gave no interaction with cupdc ions, the catalyst in the oxidising step.…”

Section: Resultsmentioning

confidence: 99%

A Method for the Determination of Noradrenaline at Normal Plasma Levels

PK¹,

Spicer²

1980

Aust J Exp Biol Med

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“…Amberlite CG-50 (Roharn and Haas) was regenerated before use by the method of Renzini, Brunori and Valori, (1970): Dowex-50 was washed and prepared for use by the method of Viktora et al (1968). dl-[7-3HI noradrenaline (specific activity 15 Ci/mmol, Amersham) was purified by batch alumina chromatography and stored in polypropylene tubes at 4'.…”

Section: Reagents and Nppnmtusmentioning

confidence: 99%

Semi‐automated Catecholamine Assay

Head

Crabb

Lande

et al. 1977

Aust J Exp Biol Med

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Summary. Three semi-automated procedures are described for the estimation of the catecholamine contents of urine, tissue and plasma samples. The three procedures are based on the fIuoromeh.ic tri-hydroxyindole assay which has been modified for automatic analysis. These techniques offer several advantages over currently available assays in that they are more convenient, provide for faster analysis rate and give increased sensitivity. The results of the present studies in which the catecholamine contents of urine, tissue and plasma samples were determined using the semi-automated assay provided a range of values which was identical to that obtained using other methods.

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A sensitive and specific fluorimetric method for the determination of noradrenalin and adrenalin in human plasma

Cited by 165 publications

References 4 publications

Raised cerebrospinal fluid norepinephrine in some patients with primary hypertension.

Raised cerebrospinal fluid norepinephrine in some patients with primary hypertension.

A Method for the Determination of Noradrenaline at Normal Plasma Levels

Semi‐automated Catecholamine Assay

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