A sensitive and specific colorimetric method for the determination of α,β-diaminopropionic acid and the Lathyrus sativus neurotoxin

Rao, Shanta S.

doi:10.1016/0003-2697(78)90762-5

Cited by 112 publications

(60 citation statements)

References 27 publications

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“…Quantitative estimation of ODAP was done by the modified method of Rao (1978). Regent; O-phthalaldehyde (OPT) reagent was prepared by mixing 100 mg OPT (dissolved in 0.2 ml mercaptoethanol), 1 ml of ethanol (95%) and 100 ml borate buffer (152.7 g potassium tetraborate and 500 ml distilled water).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Turkish grass pea (Lathyrus sativus L.) collections for it agronomic characters with a special reference to ODAP content

Başaran

Mut

Gülümser

et al. 2016

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Fifty-two grass pea (Lathyrus sativus L.) genotypes were investigated in terms of agronomic performance, protein and ODAP (b-N-oxalyl-l-diamino-propionic acid) content under semi-arid conditions of Inner Anatolia during 2013 and 2014. High variation was observed among genotypes, especially for seed yield and ODAP content. Seed yield ranged from 1142.4 kg/ha up to 2046.4 kg/ha with a mean of 1521.1 kg/ha, ODAP content in seed varied from 2.62 mg/g up to 5.59 mg/ g with an average of 3.85 mg per g and seed protein ratio was between 26.77% and 30.20% over the genotypes and years. A significant and negative correlation of ODAP was noted with seed yield (r = -0.38). This association is very encouraging for breeders to improve new varieties having high seed yield and low ODAP content. The results from this study indicated that ten grass pea genotypes consisting two ICARDA lines and eight landraces from Turkey recorded superior with low ODAP content and and high seed yield (changed between 1680.9 kg/ha and 2046.4 kg/ha). So, these may be potential candidates for new varieties.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Turkish grass pea (Lathyrus sativus L.) collections for it agronomic characters with a special reference to ODAP content

Başaran

Mut

Gülümser

et al. 2016

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“…Most of the previous analytical methods were developed for the determination of dencichine in food or seed samples [8][9][10][11][12][13]. And the recent reports about the determination of dencichine were all using the method of derivatization [4,14,15].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

Chen¹,

Yuan²,

He³

et al. 2012

AJAC

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In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra-and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.

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“…To detoxify the crop through agricultural or food processing technology, ␤-ODAP must be selectively determined-most often by using the Rao method. This method involves the alkaline hydrolysis of ␤-ODAP to L-␣,␤-diaminopropionic acid followed by reaction with orthophthalaldehyde, resulting in a colored compound that can be determined spectrophotometrically at 476 nm (9). Unfortunately, the method does not distinguish the ␤ from the ␣ form because both are hydrolysed to DAP.…”

Section: Collaborative Researchmentioning

confidence: 99%

Peer Reviewed: Analytical Chemistry in the Developing World

Solomon¹,

Åkerblom²,

Thulstrup³

2003

Anal. Chem.

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Despite some good programs and strategies to advance science in developing countries, more needs to be done. The extraction, characterization, and structural elucidation of components found in plants have been among the key research areas in many developing countries. Natural products are of interest because of the need to have a firm scientific basis for much of the herbal medicine practiced in these countries and to search for lead compounds for drug development. Most of this research, however, is classified as organic, natural product, or phytochemistry. Some developing countries have productive research groups in these areas, and they are among the leading laboratories, not only in the region, but globally. CHEMISTRY in the Developing WorldMAdvice from university-based analytical chemists is in high demand in many developing countries. Chemistry departments are relatively well established when it comes to physical and workforce resources, compared with most other laboratories in their localities. Many challenging problems are forwarded to analytical chemists at universities by government and nongovernment organizations.The pesticide residue analysis research at the University of Dar es Salaam (Tanzania) is a typical example. Tanzania exports fish from Lake Victoria to the European Union (EU), and, at one time, this was suspended because the EU required assurance that pesticide residues in the fish were below certain limits. At that time, there was no authority in Tanzania able to analyze the residues in question, so a Ph.D. student in the budding pesticide laboratory at the University of Dar es Salaam undertook this task (2). Her results were of sufficient quality that they contributed to the lifting of the EU ban on the export of Tanzanian fish. Thus, university chemistry departments often face the additional burden of providing their respective countries with services that would not normally fall within their domain elsewhere. A long list of challengesThe lack of adequate research capacity is a major weakness in many developing countries. The factors contributing to this problem are diverse and complex. As a group, developing countries differ greatly from their industrialized counterparts in their science and technology policies, financial capacities, and workforce resources. Conditions among developing countries can vary just as much.In this article, we concentrate on the major analytical challenges in sub-Saharan Africa. Some of these are poor technical support, difficulties in procuring spare parts, lack of research chemicals, shortage of capital equipment, insufficient access to funding sources, communication problems, insufficient access to and limited use of international scientific journals, weak national or regional research journals, few strong professional societies, low mobility of researchers, insufficient incentives for productive research, low salaries for university staff, and extensive educational obligations ("teaching load").Using scientific equipment in a developing country is often surp...

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A sensitive and specific colorimetric method for the determination of α,β-diaminopropionic acid and the Lathyrus sativus neurotoxin

Cited by 112 publications

References 27 publications

Evaluation of Turkish grass pea (Lathyrus sativus L.) collections for it agronomic characters with a special reference to ODAP content

Evaluation of Turkish grass pea (Lathyrus sativus L.) collections for it agronomic characters with a special reference to ODAP content

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

Peer Reviewed: Analytical Chemistry in the Developing World

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