A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes

Baeyens, Ans; Swanson, Rosemary; Herd, Olivia; Ainsbury, Elizabeth A.; Mabhengu, Thulani; Willem, Pascale; Thierens, Hubert; Slabbert, Jacobus; Vral, Anne

doi:10.3109/09553002.2011.577508

Cited by 21 publications

(21 citation statements)

References 31 publications

(49 reference statements)

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“…In the sham-irradiated sample, higher MN yields were scored with the automated method, which has also been reported in previous studies (Willems et al 2010;Baeyens et al 2011;Thierens et al 2014). The MultiBioDose study by Thierens et al (2014) In the period of time between the two intercomparisons training was offered to those labs that wanted to re-establish or improve their competence in the MN assay.…”

Section: Discussionsupporting

confidence: 67%

RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Depuydt

Baeyens

Barnard

et al. 2016

International Journal of Radiation Biology

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Purpose: In the framework of the 'Realizing the European Network of Biodosimetry' (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points ( 0.94 Gy). For higher dose points (! 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 67%

RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Depuydt

Baeyens

Barnard

et al. 2016

International Journal of Radiation Biology

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“…Selective scoring of MN that are negative for centromere-specific FISH signals can significantly improve the sensitivity to a minimum detectable acute whole body gamma-ray dose of $100 mGy for individuals [97]. First steps have been made towards the development of an automated analysis system for the micronucleus centromere assay [98] which would enable large-scale studies of cohorts exposed to low-to-moderate radiation doses. Other limitations of the CBMN assay include the minimum delay of 3 days between sampling and first results becoming available, loss of the signal with lymphocyte turnover (as discussed above for dicentrics) and its inability to detect nonuniform exposures [97].…”

Section: Micronucleimentioning

confidence: 99%

Ionizing radiation biomarkers for potential use in epidemiological studies

Pernot

Hall

Baatout

et al. 2012

Mutation Research/Reviews in Mutation Research

192

151

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Ionizing radiation is a known human carcinogen that can induce a variety of biological effects depending on the physical nature, duration, doses and dose-rates of exposure. However, the magnitude of health risks at low doses and dose-rates (below 100mSv and/or 0.1mSvmin(-1)) remains controversial due to a lack of direct human evidence. It is anticipated that significant insights will emerge from the integration of epidemiological and biological research, made possible by molecular epidemiology studies incorporating biomarkers and bioassays. A number of these have been used to investigate exposure, effects and susceptibility to ionizing radiation, albeit often at higher doses and dose rates, with each reflecting time-limited cellular or physiological alterations. This review summarises the multidisciplinary work undertaken in the framework of the European project DoReMi (Low Dose Research towards Multidisciplinary Integration) to identify the most appropriate biomarkers for use in population studies. In addition to logistical and ethical considerations for conducting large-scale epidemiological studies, we discuss the relevance of their use for assessing the effects of low dose ionizing radiation exposure at the cellular and physiological level. We also propose a temporal classification of biomarkers that may be relevant for molecular epidemiology studies which need to take into account the time elapsed since exposure. Finally, the integration of biology with epidemiology requires careful planning and enhanced discussions between the epidemiology, biology and dosimetry communities in order to determine the most important questions to be addressed in light of pragmatic considerations including the appropriate population to be investigated (occupationally, environmentally or medically exposed), and study design. The consideration of the logistics of biological sample collection, processing and storing and the choice of biomarker or bioassay, as well as awareness of potential confounding factors, are also essential.

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“…Semi-automation of MNCM -scoring (automation of micronucleus detection and manual analysis of MNCM -) enables analysis of 5000 binucleated cells in approximately 2 hours. This is longer than automation of all micronuclei seems more accurate in the low dose range (Baeyens, Swanson, 2011).…”

Section: Automation Of Micronucleus Scoringmentioning

confidence: 95%

“…When 5000 binucleated cells are scored, the detection threshold is 0.2 Gy. This scoring requires 40 min (Baeyens, 2011). Interlaboratory variability appears to be limited by the use of this software (Willems, August, 2010).…”

Section: Automation Of Micronucleus Scoringmentioning

confidence: 99%

Biological Dosimetry of Ionizing Radiation

Vaurijoux¹,

Gruel²,

Roch-Lefèvre³

et al. 2012

Current Topics in Ionizing Radiation Research

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A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes

Abstract: The semi-automated micronucleus-centromere assay combines high-speed MN analysis with a more accurate assessment in the low-dose range which makes it of special interest for large-scale radiation applications.

Cited by 21 publications

References 31 publications

RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Ionizing radiation biomarkers for potential use in epidemiological studies

Biological Dosimetry of Ionizing Radiation

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